DICP OpenIR
Subject Area分析化学
Method for liver tissue metabolic profiling based on liquid chromatography
Huang Q(黄强); Yin PY(尹沛源); Ye GZ(叶国柱); Wang J(王静); Chen J(陈静); Kong HW(孔宏伟); Lu X(路鑫); Xu GW(许国旺)
Source PublicationProceeding of HPLC 2011
Conference Name37th International Symposium on High Performance Liquid Phase Separations and Related Techniques
Conference Date2011-10-8
2011
Conference Place大连
Pages482-0
Publisher待补充
Publication Place待补充
Cooperation Status墙报
Department1808
Funding Organization中国化学会色谱专业委员会
AbstractHepatocellular carcinoma (HCC) is one of the most frequent malignancy worldwide. Evaluation of tissue metabolites is with significant value in HCC study which can provide more direct information of metabolic disorder compared with biofluids. A protocol for the metabolic profiling of liver tissue was developed based on ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC Q-TOF/MS). According to the design of experiment (DOE), methanol/water (4:1, v:v) was selected as the optimal extraction solvent. The established method was validated with a linearity over the 10–5000 ng/mL for internal standards (IS) and got an average correlation coefficient of 0.9986. The intra-day and inter-day RSD for most endogenous compounds were below 15% and the recovery of IS was from 84.8% to 109.1%. After method validation, this method was applied to HCC study. Liver tissue samples were collected from HCC patients and each sample group involved carcinoma tissue, adjacent noncancerous tissue and distal noncancerous tissue, respectively. The data demonstrated that noncancerous tissues from the adjacent and distal were nearly identical, but were greatly different from the carcinoma tissue. After the removal of missing values, totally 880 significantly changed ions between the carcinoma tissue and distal noncancerous tissue group were filtered out. 44 metabolites in the ESI positive mode and 65 in the negative mode were identified by databases and some of them were further confirmed by authentic standard samples. Several important metabolic pathways were clarified. The result proved that the established method was adequate for liver and other tissues metabolic profiling analysis.; Hepatocellular carcinoma (HCC) is one of the most frequent malignancy worldwide. Evaluation of tissue metabolites is with significant value in HCC study which can provide more direct information of metabolic disorder compared with biofluids. A protocol for the metabolic profiling of liver tissue was developed based on ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC Q-TOF/MS). According to the design of experiment (DOE), methanol/water (4:1, v:v) was selected as the optimal extraction solvent. The established method was validated with a linearity over the 10–5000 ng/mL for internal standards (IS) and got an average correlation coefficient of 0.9986. The intra-day and inter-day RSD for most endogenous compounds were below 15% and the recovery of IS was from 84.8% to 109.1%. After method validation, this method was applied to HCC study. Liver tissue samples were collected from HCC patients and each sample group involved carcinoma tissue, adjacent noncancerous tissue and distal noncancerous tissue, respectively. The data demonstrated that noncancerous tissues from the adjacent and distal were nearly identical, but were greatly different from the carcinoma tissue. After the removal of missing values, totally 880 significantly changed ions between the carcinoma tissue and distal noncancerous tissue group were filtered out. 44 metabolites in the ESI positive mode and 65 in the negative mode were identified by databases and some of them were further confirmed by authentic standard samples. Several important metabolic pathways were clarified. The result proved that the established method was adequate for liver and other tissues metabolic profiling analysis.
Document Type会议论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/116065
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺)
Recommended Citation
GB/T 7714
Huang Q,Yin PY,Ye GZ,et al. Method for liver tissue metabolic profiling based on liquid chromatography[C]. 待补充:待补充,2011:482-0.
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