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学科主题: 分析化学
题名: Metabonomic study of rheumatoid arthritis by using LC/MS and GC/MS
作者: Gu Y(谷艳) ;  ChengLu ;  QinglinZha ;  Kong HW(孔宏伟) ;  Lu X(路鑫) ;  AipingLu ;  Xu GW(许国旺)
会议文集: Proceeding of HPLC 2011
会议名称: 37th International Symposium on High Performance Liquid Phase Separations and Related Techniques
会议日期: 2011-10-8
出版日期: 2011
会议地点: 大连
通讯作者: 许国旺
出版者: 待补充
出版地: 待补充
合作性质: 墙报
部门归属: 1808
主办者: 中国化学会色谱专业委员会
摘要: Rheumatoid arthritis (RA) is a systematic autoimmune disease that causes pain, swelling, stiffness and loss of function in joints. With the rapid development of systems biology, ‘-omics’ techniques have play significant roles in the study occurrence and development of rheumatoid arthritis. Metabonomics, focusing on studying the low molecular weight metabolites in biological samples, measures the dynamic metabolic responses to pathophysiological stimuli or genetic modifications through detection and quantification of all metabolites. However, no single analytical technique can detect all of the metabolites unprejudicedly, therefore combining different platforms in order to obtain as much underlying information as possible is common in metabonomic study. In the current work, we used LC/MS and GC/MS to investigate the plasma metabolic fingerprints of RA patients compared with healthy controls, and to realize the metabolic alterations of RA. The partial least squares-discriminant analysis (PLS-DA) model showed evident separation between RA patients and healthy counterparts. The metabolic state of RA patients resulted in the increased plasma levels of carnitine, palmitoylcarnitine, saturated and mono-unsaturated phosphatidylcholine, proline, alanine, 3-hydroxybutyrate, glycerate, myoinositol, glucose, cholesterol, urea and free fatty acids, as well as decreased levels of glycochenodeoxycholic acid, lyso-phosphatidylcholine, polyunsaturated phosphatidylcholine and phosphatidylethanolamine, threonine and branched-chain amino acids. The mainly changed metabolic pathways involved in RA patients were lipid metabolism, pentose phosphate metabolism, amino acids metabolism and inositol phosphate metabolism. This study can help us understand the underlying metabolic mechanisms of RA.
英文摘要: Rheumatoid arthritis (RA) is a systematic autoimmune disease that causes pain, swelling, stiffness and loss of function in joints. With the rapid development of systems biology, ‘-omics’ techniques have play significant roles in the study occurrence and development of rheumatoid arthritis. Metabonomics, focusing on studying the low molecular weight metabolites in biological samples, measures the dynamic metabolic responses to pathophysiological stimuli or genetic modifications through detection and quantification of all metabolites. However, no single analytical technique can detect all of the metabolites unprejudicedly, therefore combining different platforms in order to obtain as much underlying information as possible is common in metabonomic study. In the current work, we used LC/MS and GC/MS to investigate the plasma metabolic fingerprints of RA patients compared with healthy controls, and to realize the metabolic alterations of RA. The partial least squares-discriminant analysis (PLS-DA) model showed evident separation between RA patients and healthy counterparts. The metabolic state of RA patients resulted in the increased plasma levels of carnitine, palmitoylcarnitine, saturated and mono-unsaturated phosphatidylcholine, proline, alanine, 3-hydroxybutyrate, glycerate, myoinositol, glucose, cholesterol, urea and free fatty acids, as well as decreased levels of glycochenodeoxycholic acid, lyso-phosphatidylcholine, polyunsaturated phosphatidylcholine and phosphatidylethanolamine, threonine and branched-chain amino acids. The mainly changed metabolic pathways involved in RA patients were lipid metabolism, pentose phosphate metabolism, amino acids metabolism and inositol phosphate metabolism. This study can help us understand the underlying metabolic mechanisms of RA.
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116066
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Gu Y,ChengLu,QinglinZha,et al. Metabonomic study of rheumatoid arthritis by using LC/MS and GC/MS[C]. 见:37th International Symposium on High Performance Liquid Phase Separations and Related Techniques. 大连. 2011-10-8.
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