DICP OpenIR
Subject Area分析化学
Development and validation of a method for qualification and quantification of free amino acids in rice
Zhang JJ(张俊杰); Chang YW(常玉玮); Zhao CX(赵春霞); Zhou J(周佳); Lu X(路鑫); Xu GW(许国旺)
Source PublicationProceeding of HPLC 2011
Conference Name37th International Symposium on High Performance Liquid Phase Separations and Related Techniques
Conference Date2011-10-8
2011
Conference Place大连
Pages695-0
Publisher待补充
Publication Place待补充
Cooperation Status墙报
Department1808
Funding Organization中国化学会色谱专业委员会
AbstractFree amino acids contents are very important information for studying the genotype, grow condition and environment of plants, and have been detected with many different methods. Here a profiling method with reverse phase-ultra high performance liquid chromatography (UHPLC) coupled with quadrupole time of flight (qTOF) mass spectrometry after 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivation was applied and evaluated to qualify and quantify free amino acids in rice grains. 28 kinds of amino acids have been identified and 21 of them were quantified simultaneously. Evaluated result showed that quantified 21 kinds of amino acids have good calibration range, a recovery between 67.93%-118.17%, intra repetition 3.16%-19.6%(n=5), inter repetition1.05%-19.53% (n=4). Wild rices with different sowing time or sites and gene-modified rice introduced crylAc and sck genes were analyzed to evaluate the influence of environment and genotype. Non-parameter test showed that most of the free amino acids contents of transgenic rice are different from the wild rice. It was found that both environment and gene modification have remarkable effects on free amino acids contents of rice grains.; Free amino acids contents are very important information for studying the genotype, grow condition and environment of plants, and have been detected with many different methods. Here a profiling method with reverse phase-ultra high performance liquid chromatography (UHPLC) coupled with quadrupole time of flight (qTOF) mass spectrometry after 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivation was applied and evaluated to qualify and quantify free amino acids in rice grains. 28 kinds of amino acids have been identified and 21 of them were quantified simultaneously. Evaluated result showed that quantified 21 kinds of amino acids have good calibration range, a recovery between 67.93%-118.17%, intra repetition 3.16%-19.6%(n=5), inter repetition1.05%-19.53% (n=4). Wild rices with different sowing time or sites and gene-modified rice introduced crylAc and sck genes were analyzed to evaluate the influence of environment and genotype. Non-parameter test showed that most of the free amino acids contents of transgenic rice are different from the wild rice. It was found that both environment and gene modification have remarkable effects on free amino acids contents of rice grains.
Document Type会议论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/116080
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺)
Recommended Citation
GB/T 7714
Zhang JJ,Chang YW,Zhao CX,et al. Development and validation of a method for qualification and quantification of free amino acids in rice[C]. 待补充:待补充,2011:695-0.
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