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学科主题: 分析化学
题名: Development and validation of a method for qualification and quantification of free amino acids in rice
作者: Zhang JJ(张俊杰) ;  Chang YW(常玉玮) ;  Zhao CX(赵春霞) ;  Zhou J(周佳) ;  Lu X(路鑫) ;  Xu GW(许国旺)
会议文集: Proceeding of HPLC 2011
会议名称: 37th International Symposium on High Performance Liquid Phase Separations and Related Techniques
会议日期: 2011-10-8
出版日期: 2011
会议地点: 大连
通讯作者: 许国旺
出版者: 待补充
出版地: 待补充
合作性质: 墙报
部门归属: 1808
主办者: 中国化学会色谱专业委员会
摘要: Free amino acids contents are very important information for studying the genotype, grow condition and environment of plants, and have been detected with many different methods. Here a profiling method with reverse phase-ultra high performance liquid chromatography (UHPLC) coupled with quadrupole time of flight (qTOF) mass spectrometry after 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivation was applied and evaluated to qualify and quantify free amino acids in rice grains. 28 kinds of amino acids have been identified and 21 of them were quantified simultaneously. Evaluated result showed that quantified 21 kinds of amino acids have good calibration range, a recovery between 67.93%-118.17%, intra repetition 3.16%-19.6%(n=5), inter repetition1.05%-19.53% (n=4). Wild rices with different sowing time or sites and gene-modified rice introduced crylAc and sck genes were analyzed to evaluate the influence of environment and genotype. Non-parameter test showed that most of the free amino acids contents of transgenic rice are different from the wild rice. It was found that both environment and gene modification have remarkable effects on free amino acids contents of rice grains.
英文摘要: Free amino acids contents are very important information for studying the genotype, grow condition and environment of plants, and have been detected with many different methods. Here a profiling method with reverse phase-ultra high performance liquid chromatography (UHPLC) coupled with quadrupole time of flight (qTOF) mass spectrometry after 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivation was applied and evaluated to qualify and quantify free amino acids in rice grains. 28 kinds of amino acids have been identified and 21 of them were quantified simultaneously. Evaluated result showed that quantified 21 kinds of amino acids have good calibration range, a recovery between 67.93%-118.17%, intra repetition 3.16%-19.6%(n=5), inter repetition1.05%-19.53% (n=4). Wild rices with different sowing time or sites and gene-modified rice introduced crylAc and sck genes were analyzed to evaluate the influence of environment and genotype. Non-parameter test showed that most of the free amino acids contents of transgenic rice are different from the wild rice. It was found that both environment and gene modification have remarkable effects on free amino acids contents of rice grains.
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116080
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Zhang JJ,Chang YW,Zhao CX,et al. Development and validation of a method for qualification and quantification of free amino acids in rice[C]. 见:37th International Symposium on High Performance Liquid Phase Separations and Related Techniques. 大连. 2011-10-8.
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