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学科主题分析化学
Serum lipid profiling of patients with chronic hepatitis B, cirrhosis and hepatocellular carcinoma by ultra fast liquid chromatography/ion trap-time of flight mass spectrometry
Chen SL(陈世礼); Yin PY(尹沛源); Zhao XJ(赵欣捷); WenbinXing; Hu CX(胡春秀); Zhou LN(周丽娜); Xu GW(许国旺)
会议文集Proceeding of HPLC 2011
会议名称37th International Symposium on High Performance Liquid Phase Separations and Related Techniques
会议日期2011-10-8
2011
会议地点大连
页码721-0
出版者待补充
出版地待补充
合作性质墙报
部门归属1808
主办者中国化学会色谱专业委员会
英文摘要An ultra fast liquid chromatography/ion trap-time of flight mass spectrometry (UFLC/MS-IT-TOF) based serum lipidomics platform was developed to characterize the serum lipid profile of patients with Chronic Hepatitis B (CHB), Cirrhosis, Hepatocellular Carcinoma (HCC) and normal controls. CH3OH/CH2Cl2/H2O solvent mixture was employed to extract lipids from serum, which was simple and reproducible. Reversed-phase liquid chromatography was used to separate the lipids according to their hydrophobicity, possessing the advantage of retention time stability than normal-phase liquid chromatography. Detection was performed on Ion Trap-Time of Flight Mass Spectrometry, which provided high sensitivity to detect low-content lipid component and high accurate m/z to facilitate lipid identification. After data collection and processing, 96 lipids within five species were identified including lysophosphatidylcholines, phosphatidylcholines, sphingomyelins, triacylglycerides and cholesterol esters. Heatmap and multivariate analysis methods PCA and PLS-DA were used to overview the lipid profile relationship between CHB, Cirrhosis, HCC patients and normal controls. CHB patients and healthy controls had similar serum lipid profile, but some differences still existed. Cirrhosis and HCC patients had similar serum lipid profile, and both were different from normal controls or even CHB patients. When ANOVA test was performed, Cirrhosis and HCC patients had many significantly decreased serum lipids compared with CHB patients or the controls. CHB also induced several serum lipids significantly altered in patients compared with normal controls, however very few lipid metabolites were differently expressed in HCC when compared with Cirrhosis patients. The univariate analysis results confirmed the heatmap and multivariate results, and all indicated the mild liver injury in CHB patients and severe injury severity in Cirrhosis and HCC patients. This work demonstrated the utility of LC/MS-based serum lipidomics platform as a powerful tool to investigate the pathogenesis and development of HBV-related liver diseases.; An ultra fast liquid chromatography/ion trap-time of flight mass spectrometry (UFLC/MS-IT-TOF) based serum lipidomics platform was developed to characterize the serum lipid profile of patients with Chronic Hepatitis B (CHB), Cirrhosis, Hepatocellular Carcinoma (HCC) and normal controls. CH3OH/CH2Cl2/H2O solvent mixture was employed to extract lipids from serum, which was simple and reproducible. Reversed-phase liquid chromatography was used to separate the lipids according to their hydrophobicity, possessing the advantage of retention time stability than normal-phase liquid chromatography. Detection was performed on Ion Trap-Time of Flight Mass Spectrometry, which provided high sensitivity to detect low-content lipid component and high accurate m/z to facilitate lipid identification. After data collection and processing, 96 lipids within five species were identified including lysophosphatidylcholines, phosphatidylcholines, sphingomyelins, triacylglycerides and cholesterol esters. Heatmap and multivariate analysis methods PCA and PLS-DA were used to overview the lipid profile relationship between CHB, Cirrhosis, HCC patients and normal controls. CHB patients and healthy controls had similar serum lipid profile, but some differences still existed. Cirrhosis and HCC patients had similar serum lipid profile, and both were different from normal controls or even CHB patients. When ANOVA test was performed, Cirrhosis and HCC patients had many significantly decreased serum lipids compared with CHB patients or the controls. CHB also induced several serum lipids significantly altered in patients compared with normal controls, however very few lipid metabolites were differently expressed in HCC when compared with Cirrhosis patients. The univariate analysis results confirmed the heatmap and multivariate results, and all indicated the mild liver injury in CHB patients and severe injury severity in Cirrhosis and HCC patients. This work demonstrated the utility of LC/MS-based serum lipidomics platform as a powerful tool to investigate the pathogenesis and development of HBV-related liver diseases.
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/116081
专题中国科学院大连化学物理研究所
通讯作者Xu GW(许国旺)
推荐引用方式
GB/T 7714
Chen SL,Yin PY,Zhao XJ,et al. Serum lipid profiling of patients with chronic hepatitis B, cirrhosis and hepatocellular carcinoma by ultra fast liquid chromatography/ion trap-time of flight mass spectrometry[C]. 待补充:待补充,2011:721-0.
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