中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 分析化学
题名: Serum lipid profiling of patients with chronic hepatitis B, cirrhosis and hepatocellular carcinoma by ultra fast liquid chromatography/ion trap-time of flight mass spectrometry
作者: Chen SL(陈世礼) ;  Yin PY(尹沛源) ;  Zhao XJ(赵欣捷) ;  WenbinXing ;  Hu CX(胡春秀) ;  Zhou LN(周丽娜) ;  Xu GW(许国旺)
会议文集: Proceeding of HPLC 2011
会议名称: 37th International Symposium on High Performance Liquid Phase Separations and Related Techniques
会议日期: 2011-10-8
出版日期: 2011
会议地点: 大连
通讯作者: 许国旺
出版者: 待补充
出版地: 待补充
合作性质: 墙报
部门归属: 1808
主办者: 中国化学会色谱专业委员会
摘要: An ultra fast liquid chromatography/ion trap-time of flight mass spectrometry (UFLC/MS-IT-TOF) based serum lipidomics platform was developed to characterize the serum lipid profile of patients with Chronic Hepatitis B (CHB), Cirrhosis, Hepatocellular Carcinoma (HCC) and normal controls. CH3OH/CH2Cl2/H2O solvent mixture was employed to extract lipids from serum, which was simple and reproducible. Reversed-phase liquid chromatography was used to separate the lipids according to their hydrophobicity, possessing the advantage of retention time stability than normal-phase liquid chromatography. Detection was performed on Ion Trap-Time of Flight Mass Spectrometry, which provided high sensitivity to detect low-content lipid component and high accurate m/z to facilitate lipid identification. After data collection and processing, 96 lipids within five species were identified including lysophosphatidylcholines, phosphatidylcholines, sphingomyelins, triacylglycerides and cholesterol esters. Heatmap and multivariate analysis methods PCA and PLS-DA were used to overview the lipid profile relationship between CHB, Cirrhosis, HCC patients and normal controls. CHB patients and healthy controls had similar serum lipid profile, but some differences still existed. Cirrhosis and HCC patients had similar serum lipid profile, and both were different from normal controls or even CHB patients. When ANOVA test was performed, Cirrhosis and HCC patients had many significantly decreased serum lipids compared with CHB patients or the controls. CHB also induced several serum lipids significantly altered in patients compared with normal controls, however very few lipid metabolites were differently expressed in HCC when compared with Cirrhosis patients. The univariate analysis results confirmed the heatmap and multivariate results, and all indicated the mild liver injury in CHB patients and severe injury severity in Cirrhosis and HCC patients. This work demonstrated the utility of LC/MS-based serum lipidomics platform as a powerful tool to investigate the pathogenesis and development of HBV-related liver diseases.
英文摘要: An ultra fast liquid chromatography/ion trap-time of flight mass spectrometry (UFLC/MS-IT-TOF) based serum lipidomics platform was developed to characterize the serum lipid profile of patients with Chronic Hepatitis B (CHB), Cirrhosis, Hepatocellular Carcinoma (HCC) and normal controls. CH3OH/CH2Cl2/H2O solvent mixture was employed to extract lipids from serum, which was simple and reproducible. Reversed-phase liquid chromatography was used to separate the lipids according to their hydrophobicity, possessing the advantage of retention time stability than normal-phase liquid chromatography. Detection was performed on Ion Trap-Time of Flight Mass Spectrometry, which provided high sensitivity to detect low-content lipid component and high accurate m/z to facilitate lipid identification. After data collection and processing, 96 lipids within five species were identified including lysophosphatidylcholines, phosphatidylcholines, sphingomyelins, triacylglycerides and cholesterol esters. Heatmap and multivariate analysis methods PCA and PLS-DA were used to overview the lipid profile relationship between CHB, Cirrhosis, HCC patients and normal controls. CHB patients and healthy controls had similar serum lipid profile, but some differences still existed. Cirrhosis and HCC patients had similar serum lipid profile, and both were different from normal controls or even CHB patients. When ANOVA test was performed, Cirrhosis and HCC patients had many significantly decreased serum lipids compared with CHB patients or the controls. CHB also induced several serum lipids significantly altered in patients compared with normal controls, however very few lipid metabolites were differently expressed in HCC when compared with Cirrhosis patients. The univariate analysis results confirmed the heatmap and multivariate results, and all indicated the mild liver injury in CHB patients and severe injury severity in Cirrhosis and HCC patients. This work demonstrated the utility of LC/MS-based serum lipidomics platform as a powerful tool to investigate the pathogenesis and development of HBV-related liver diseases.
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116081
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Chen SL,Yin PY,Zhao XJ,et al. Serum lipid profiling of patients with chronic hepatitis B, cirrhosis and hepatocellular carcinoma by ultra fast liquid chromatography/ion trap-time of flight mass spectrometry[C]. 见:37th International Symposium on High Performance Liquid Phase Separations and Related Techniques. 大连. 2011-10-8.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [陈世礼]'s Articles
 [尹沛源]'s Articles
 [赵欣捷]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [陈世礼]‘s Articles
 [尹沛源]‘s Articles
 [赵欣捷]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace