DICP OpenIR
Subject Area生物化学
Chitan oligosaccharide and salicylic acid differently activate arteminisin production and gene expression in Artemisia annua
Yin H(尹恒); AndersKjar; Du YG(杜昱光); KaiGrevsen; YuguangDu
Source PublicationBook of Abstracts
Conference NameAsian Congress on Biotechnology
Conference Date2011-5-11
2011
Conference Place上海
Pages80-0
Publisher待补充
Publication Place待补充
Cooperation Status分会口头报告
Department1805
Funding Organization亚洲生物技术联合会(AFOB)、上海交通大学、华东理工大学、湖北工业大学、山东大学
AbstractArtemisia annua L. is a traditional Chinese medicinal plant used for treatment of malaria. Artemisinin which accumulated in the glandular trichomes of this plant is the primary anti-malaria component of the Artemisia annua plant extraction. Due to the great importance of artemisinin, lots of efforts have been made to improve artemisinin production in plants. In this study, the effect of chitosan oligosaccharide (a plant secondary metabolites production elicitor which is hydrolyzed from chitosan) and salicylic acid (SA) both on arteminisin production and arteminisin biosynthesis related gene expression were investiged. HPLC analysis showed that both chitosan oligosaccharide and SA treatment can induce artemisinin production. At the same time, the influence of these treatments on expression of biosynthetic genes was studied by RT-PCR technique. The transcriptional levels of some related genes like ADS, CPR, CYP71AV1 were up-regulated by chitosan oligosaccharide and SA, but the inducing patterns were different. In our knowledge, this is the first report about the chitosan oligosaccharide effect on artemisinin generation in Artemisia annua plant. The result suggests that chitosan oligosaccharide can induce artemisinin accumulation and reveal a new method to promote artemisinin yield in field production.; Artemisia annua L. is a traditional Chinese medicinal plant used for treatment of malaria. Artemisinin which accumulated in the glandular trichomes of this plant is the primary anti-malaria component of the Artemisia annua plant extraction. Due to the great importance of artemisinin, lots of efforts have been made to improve artemisinin production in plants. In this study, the effect of chitosan oligosaccharide (a plant secondary metabolites production elicitor which is hydrolyzed from chitosan) and salicylic acid (SA) both on arteminisin production and arteminisin biosynthesis related gene expression were investiged. HPLC analysis showed that both chitosan oligosaccharide and SA treatment can induce artemisinin production. At the same time, the influence of these treatments on expression of biosynthetic genes was studied by RT-PCR technique. The transcriptional levels of some related genes like ADS, CPR, CYP71AV1 were up-regulated by chitosan oligosaccharide and SA, but the inducing patterns were different. In our knowledge, this is the first report about the chitosan oligosaccharide effect on artemisinin generation in Artemisia annua plant. The result suggests that chitosan oligosaccharide can induce artemisinin accumulation and reveal a new method to promote artemisinin yield in field production.
Document Type会议论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/116083
Collection中国科学院大连化学物理研究所
Corresponding AuthorYuguangDu
Recommended Citation
GB/T 7714
Yin H,AndersKjar,Du YG,et al. Chitan oligosaccharide and salicylic acid differently activate arteminisin production and gene expression in Artemisia annua[C]. 待补充:待补充,2011:80-0.
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