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学科主题: 生物化学
题名: Chitan oligosaccharide and salicylic acid differently activate arteminisin production and gene expression in Artemisia annua
作者: Yin H(尹恒) ;  AndersKjar ;  Du YG(杜昱光) ;  KaiGrevsen
会议文集: Book of Abstracts
会议名称: Asian Congress on Biotechnology
会议日期: 2011-5-11
出版日期: 2011
会议地点: 上海
通讯作者: YuguangDu
出版者: 待补充
出版地: 待补充
合作性质: 分会口头报告
部门归属: 1805
主办者: 亚洲生物技术联合会(AFOB)、上海交通大学、华东理工大学、湖北工业大学、山东大学
摘要: Artemisia annua L. is a traditional Chinese medicinal plant used for treatment of malaria. Artemisinin which accumulated in the glandular trichomes of this plant is the primary anti-malaria component of the Artemisia annua plant extraction. Due to the great importance of artemisinin, lots of efforts have been made to improve artemisinin production in plants. In this study, the effect of chitosan oligosaccharide (a plant secondary metabolites production elicitor which is hydrolyzed from chitosan) and salicylic acid (SA) both on arteminisin production and arteminisin biosynthesis related gene expression were investiged. HPLC analysis showed that both chitosan oligosaccharide and SA treatment can induce artemisinin production. At the same time, the influence of these treatments on expression of biosynthetic genes was studied by RT-PCR technique. The transcriptional levels of some related genes like ADS, CPR, CYP71AV1 were up-regulated by chitosan oligosaccharide and SA, but the inducing patterns were different. In our knowledge, this is the first report about the chitosan oligosaccharide effect on artemisinin generation in Artemisia annua plant. The result suggests that chitosan oligosaccharide can induce artemisinin accumulation and reveal a new method to promote artemisinin yield in field production.
英文摘要: Artemisia annua L. is a traditional Chinese medicinal plant used for treatment of malaria. Artemisinin which accumulated in the glandular trichomes of this plant is the primary anti-malaria component of the Artemisia annua plant extraction. Due to the great importance of artemisinin, lots of efforts have been made to improve artemisinin production in plants. In this study, the effect of chitosan oligosaccharide (a plant secondary metabolites production elicitor which is hydrolyzed from chitosan) and salicylic acid (SA) both on arteminisin production and arteminisin biosynthesis related gene expression were investiged. HPLC analysis showed that both chitosan oligosaccharide and SA treatment can induce artemisinin production. At the same time, the influence of these treatments on expression of biosynthetic genes was studied by RT-PCR technique. The transcriptional levels of some related genes like ADS, CPR, CYP71AV1 were up-regulated by chitosan oligosaccharide and SA, but the inducing patterns were different. In our knowledge, this is the first report about the chitosan oligosaccharide effect on artemisinin generation in Artemisia annua plant. The result suggests that chitosan oligosaccharide can induce artemisinin accumulation and reveal a new method to promote artemisinin yield in field production.
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116083
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Yin H,AndersKjar,Du YG,et al. Chitan oligosaccharide and salicylic acid differently activate arteminisin production and gene expression in Artemisia annua[C]. 见:Asian Congress on Biotechnology. 上海. 2011-5-11.
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