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学科主题: 生物化学
题名: Isolation and Structural Characterization of a Polysaccharide LRGP1 from the Fruit of Lycium Ruthenicum Murr.
作者: Peng Q(彭强) ;  Li Y(李昱) ;  Xu QS(许青松) ;  Du YG(杜昱光)
会议文集: Asian Communications of Glycobiology and Glycotechnology
会议名称: The 3rd Asian Communications of Glycobiology and Glycotechnology
会议日期: 2011-10-27
出版日期: 2011
会议地点: 上海
其他题名: 黑果枸杞多糖组分1的分离与结构鉴定
通讯作者: YuguangDu
出版者: 待补充
出版地: 待补充
合作性质: 墙报
部门归属: 1805
主办者: 中国科学院上海有机化学所
摘要: Lycium ruthenicum Murr., is a famous traditional Chinese herbal medicine which widely distributes in salinized desert of Qinghai-Tibet Plateau. Its ripe fruits had been used for treatment of heart disease, abnormal menstruation and menopause. Likewise, polysaccharides isolated from L. ruthenicum were shown to have antidiabetic and antifatigue effects. But up to now no comprehensive study has been conducted to explore the structural characterization of L.ruthenicum polysaccharides. This has largely restricted the further research and development of L. ruthenicum. In this study, the crude polysaccharide was extracted following the usual separation procedure, including water dissolution, centrifugation and precipitation with ethanol. The crude polysaccharide was then applied to anion-exchange chromatographyand gel filtration to obtain a homogeneous polysaccharide, designated as LRGP1. Its molecular weight was 56.2 kDa determined by HPGPC. Monosaccharide composition analysis revealed that it was composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 4:48:1:2.2:3.7:41. LRGP1 had IR bands at 800-1200cm−1, 1450-1800cm−1, 2500-3000cm−1, and 3200-3600cm−1, which were distinctive absorptions of polysaccharides. On the basis of total acid hydrolysis, partial acid hydrolysis, methylation and ESI-MS analysis, LRGP1 was a branched polysaccharide rich in arabinose and galactose with a backbone composed of →6)-Gal-(1→. The branches were composed of (1→5)-linked- Ara, (1→2)-linked-Ara, (1→2,4)-linked-Rha and (1→3)-linked Gal.Arabinose, xylose, mannose, and glucose were located at the terminal of the branches. This is the first report on structural characterizetion of polysaccharide from L. ruthenicum.
英文摘要: Lycium ruthenicum Murr., is a famous traditional Chinese herbal medicine which widely distributes in salinized desert of Qinghai-Tibet Plateau. Its ripe fruits had been used for treatment of heart disease, abnormal menstruation and menopause. Likewise, polysaccharides isolated from L. ruthenicum were shown to have antidiabetic and antifatigue effects. But up to now no comprehensive study has been conducted to explore the structural characterization of L.ruthenicum polysaccharides. This has largely restricted the further research and development of L. ruthenicum. In this study, the crude polysaccharide was extracted following the usual separation procedure, including water dissolution, centrifugation and precipitation with ethanol. The crude polysaccharide was then applied to anion-exchange chromatographyand gel filtration to obtain a homogeneous polysaccharide, designated as LRGP1. Its molecular weight was 56.2 kDa determined by HPGPC. Monosaccharide composition analysis revealed that it was composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 4:48:1:2.2:3.7:41. LRGP1 had IR bands at 800-1200cm−1, 1450-1800cm−1, 2500-3000cm−1, and 3200-3600cm−1, which were distinctive absorptions of polysaccharides. On the basis of total acid hydrolysis, partial acid hydrolysis, methylation and ESI-MS analysis, LRGP1 was a branched polysaccharide rich in arabinose and galactose with a backbone composed of →6)-Gal-(1→. The branches were composed of (1→5)-linked- Ara, (1→2)-linked-Ara, (1→2,4)-linked-Rha and (1→3)-linked Gal.Arabinose, xylose, mannose, and glucose were located at the terminal of the branches. This is the first report on structural characterizetion of polysaccharide from L. ruthenicum.
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116086
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Peng Q,Li Y,Xu QS,et al. Isolation and Structural Characterization of a Polysaccharide LRGP1 from the Fruit of Lycium Ruthenicum Murr.[C]. 见:The 3rd Asian Communications of Glycobiology and Glycotechnology. 上海. 2011-10-27.
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