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学科主题生物化学
Isolation and Structural Characterization of a Polysaccharide LRGP1 from the Fruit of Lycium Ruthenicum Murr.
Peng Q(彭强); Li Y(李昱); Xu QS(许青松); Du YG(杜昱光); YuguangDu
会议文集Asian Communications of Glycobiology and Glycotechnology
会议名称The 3rd Asian Communications of Glycobiology and Glycotechnology
会议日期2011-10-27
2011
会议地点上海
其他题名黑果枸杞多糖组分1的分离与结构鉴定
页码70-0
出版者待补充
出版地待补充
合作性质墙报
部门归属1805
主办者中国科学院上海有机化学所
英文摘要Lycium ruthenicum Murr., is a famous traditional Chinese herbal medicine which widely distributes in salinized desert of Qinghai-Tibet Plateau. Its ripe fruits had been used for treatment of heart disease, abnormal menstruation and menopause. Likewise, polysaccharides isolated from L. ruthenicum were shown to have antidiabetic and antifatigue effects. But up to now no comprehensive study has been conducted to explore the structural characterization of L.ruthenicum polysaccharides. This has largely restricted the further research and development of L. ruthenicum. In this study, the crude polysaccharide was extracted following the usual separation procedure, including water dissolution, centrifugation and precipitation with ethanol. The crude polysaccharide was then applied to anion-exchange chromatographyand gel filtration to obtain a homogeneous polysaccharide, designated as LRGP1. Its molecular weight was 56.2 kDa determined by HPGPC. Monosaccharide composition analysis revealed that it was composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 4:48:1:2.2:3.7:41. LRGP1 had IR bands at 800-1200cm−1, 1450-1800cm−1, 2500-3000cm−1, and 3200-3600cm−1, which were distinctive absorptions of polysaccharides. On the basis of total acid hydrolysis, partial acid hydrolysis, methylation and ESI-MS analysis, LRGP1 was a branched polysaccharide rich in arabinose and galactose with a backbone composed of →6)-Gal-(1→. The branches were composed of (1→5)-linked- Ara, (1→2)-linked-Ara, (1→2,4)-linked-Rha and (1→3)-linked Gal.Arabinose, xylose, mannose, and glucose were located at the terminal of the branches. This is the first report on structural characterizetion of polysaccharide from L. ruthenicum.; Lycium ruthenicum Murr., is a famous traditional Chinese herbal medicine which widely distributes in salinized desert of Qinghai-Tibet Plateau. Its ripe fruits had been used for treatment of heart disease, abnormal menstruation and menopause. Likewise, polysaccharides isolated from L. ruthenicum were shown to have antidiabetic and antifatigue effects. But up to now no comprehensive study has been conducted to explore the structural characterization of L.ruthenicum polysaccharides. This has largely restricted the further research and development of L. ruthenicum. In this study, the crude polysaccharide was extracted following the usual separation procedure, including water dissolution, centrifugation and precipitation with ethanol. The crude polysaccharide was then applied to anion-exchange chromatographyand gel filtration to obtain a homogeneous polysaccharide, designated as LRGP1. Its molecular weight was 56.2 kDa determined by HPGPC. Monosaccharide composition analysis revealed that it was composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 4:48:1:2.2:3.7:41. LRGP1 had IR bands at 800-1200cm−1, 1450-1800cm−1, 2500-3000cm−1, and 3200-3600cm−1, which were distinctive absorptions of polysaccharides. On the basis of total acid hydrolysis, partial acid hydrolysis, methylation and ESI-MS analysis, LRGP1 was a branched polysaccharide rich in arabinose and galactose with a backbone composed of →6)-Gal-(1→. The branches were composed of (1→5)-linked- Ara, (1→2)-linked-Ara, (1→2,4)-linked-Rha and (1→3)-linked Gal.Arabinose, xylose, mannose, and glucose were located at the terminal of the branches. This is the first report on structural characterizetion of polysaccharide from L. ruthenicum.
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/116086
专题中国科学院大连化学物理研究所
通讯作者YuguangDu
推荐引用方式
GB/T 7714
Peng Q,Li Y,Xu QS,et al. Isolation and Structural Characterization of a Polysaccharide LRGP1 from the Fruit of Lycium Ruthenicum Murr.[C]. 待补充:待补充,2011:70-0.
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