中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 生物化学
题名: Roles of N-acetylglucosaminyltransferase III in Epithelial-to-mesenchymal Transition Induced by TGF-β1
作者: Xu QS(许青松) ;  Gu JG(顾建国) ;  Du YG(杜昱光)
会议文集: Asian Communications of Glycobiology and Glycotechnology
会议名称: The 3rd Asian Communications of Glycobiology and Glycotechnology
会议日期: 2011-10-27
出版日期: 2011
会议地点: 上海
其他题名: N-乙酰氨基葡萄糖转移酶III在转化生长因子β1诱导的上皮间质转化中的作用
通讯作者: YuguangDu
出版者: 待补充
出版地: 待补充
合作性质: 墙报
部门归属: 1805
主办者: 中国科学院上海有机化学所
摘要: The epithelial-to-mesenchymal transition (EMT) plays crucial roles in the embryonic development, wound healing, tissue repair and cancer progression. In the present study, we demonstrate that transforming growth factor β (TGF-β) down-regulated expression of N-acetylglucosaminyltransferase III (GnT-III) during EMT. The treatment with TGF-β1 resulted in a decrease in E-cadherin expression and GnT-III expression as well as its product, the bisected N-glycans, which was confirmed by E4-PHA lectin blot in human MCF-10A and mouse GE11 cells. To understand roles of GnT-III expression in EMT, the MCF-10A cell was stably transfected with GnT-III. Of particular interesting, overexpression of GnT-III partially, but did inhibit EMT induced by TGF-β1, which were confirmed by cell morphological changes of phase-contrast, immunochemical staining patterns of E-cadherin and actin. In addition, GnT-III modified E-cadherin, which contributed to prolong E-cadherin turnover on cell surface examined by biotinylation and pulse-chase experiments. Consistently, GnT-III expression inhibited β-catenin translocation from cell-cell contact into cytoplasm and nucleus. Furthermore, the transwell assay showed that GnT-III expression suppresses TGF-β-induced cell motility. Taken together, these observations are the first to clearly demonstrate that GnT-III plays important roles in EMT, and explain a molecular mechanism for inhibitory effects of GnT-III on cancer metastasis.
英文摘要: The epithelial-to-mesenchymal transition (EMT) plays crucial roles in the embryonic development, wound healing, tissue repair and cancer progression. In the present study, we demonstrate that transforming growth factor β (TGF-β) down-regulated expression of N-acetylglucosaminyltransferase III (GnT-III) during EMT. The treatment with TGF-β1 resulted in a decrease in E-cadherin expression and GnT-III expression as well as its product, the bisected N-glycans, which was confirmed by E4-PHA lectin blot in human MCF-10A and mouse GE11 cells. To understand roles of GnT-III expression in EMT, the MCF-10A cell was stably transfected with GnT-III. Of particular interesting, overexpression of GnT-III partially, but did inhibit EMT induced by TGF-β1, which were confirmed by cell morphological changes of phase-contrast, immunochemical staining patterns of E-cadherin and actin. In addition, GnT-III modified E-cadherin, which contributed to prolong E-cadherin turnover on cell surface examined by biotinylation and pulse-chase experiments. Consistently, GnT-III expression inhibited β-catenin translocation from cell-cell contact into cytoplasm and nucleus. Furthermore, the transwell assay showed that GnT-III expression suppresses TGF-β-induced cell motility. Taken together, these observations are the first to clearly demonstrate that GnT-III plays important roles in EMT, and explain a molecular mechanism for inhibitory effects of GnT-III on cancer metastasis.
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116087
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Xu QS,Gu JG,Du YG. Roles of N-acetylglucosaminyltransferase III in Epithelial-to-mesenchymal Transition Induced by TGF-β1[C]. 见:The 3rd Asian Communications of Glycobiology and Glycotechnology. 上海. 2011-10-27.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [许青松]'s Articles
 [顾建国]'s Articles
 [杜昱光]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [许青松]‘s Articles
 [顾建国]‘s Articles
 [杜昱光]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace