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学科主题: 生物化学
题名: Chito-oligosaccharides Inhibit Production of Nitric Oxide in Lipopolysaccharide-Induced N9 Murine Microglial Cells
作者: Wei P(魏鹏) ;  Xu QS(许青松) ;  Du YG(杜昱光) ;  Yu C(于超)
会议文集: Asian Communications of Glycobiology and Glycotechnology
会议名称: The 3rd Asian Communications of Glycobiology and Glycotechnology
会议日期: 2011-10-27
出版日期: 2011
会议地点: 上海
其他题名: 壳寡糖在N9小胶质细胞中抑制由脂多糖诱导的一氧化氮的生成
通讯作者: 杜昱光
出版者: 待补充
出版地: 待补充
合作性质: 墙报
部门归属: 1805
主办者: 中国科学院上海有机化学所
摘要: Chito-oligosaccharides (COS) have been reported to exert many biological activities, such as antioxidant, antitumor and anti-inflammatory effects. However, the effects of COS on inflammatory microglial cells remain unknown. In present study, we examined the effect of COS on nitric oxide (NO) production in lipopolysaccharides (LPS) induced N9 microglial cells. Pretreatment with COS (50~200μg/ml) could markedly inhibit NO and inducible nitric oxide synthase (iNOS) production in LPS-induced N9 microglial cells. Signal transduction studies showed that COS remarkably inhibited LPS-induced phosphorylation of p38 mitogen-activated protein kinase (MAPK) and ERK1/2. Transcription factors NF-κB and AP-1 in N9 microglial cells were also significantly inhibited by pretreatment with COS for 24h. The anti-inflammatory effect of COS was comparable to that of positive control (hydrocortisone, 1M). In conclusion, our results suggest that the inhibitory effect of COS on the production of NO and iNOS in LPS-induced N9 microglial cells is mediated by both NF-κB and AP-1 dependent pathways. COS could also suppress the phosphorylation of p38 MAPK and ERK1/2.
英文摘要: Chito-oligosaccharides (COS) have been reported to exert many biological activities, such as antioxidant, antitumor and anti-inflammatory effects. However, the effects of COS on inflammatory microglial cells remain unknown. In present study, we examined the effect of COS on nitric oxide (NO) production in lipopolysaccharides (LPS) induced N9 microglial cells. Pretreatment with COS (50~200μg/ml) could markedly inhibit NO and inducible nitric oxide synthase (iNOS) production in LPS-induced N9 microglial cells. Signal transduction studies showed that COS remarkably inhibited LPS-induced phosphorylation of p38 mitogen-activated protein kinase (MAPK) and ERK1/2. Transcription factors NF-κB and AP-1 in N9 microglial cells were also significantly inhibited by pretreatment with COS for 24h. The anti-inflammatory effect of COS was comparable to that of positive control (hydrocortisone, 1M). In conclusion, our results suggest that the inhibitory effect of COS on the production of NO and iNOS in LPS-induced N9 microglial cells is mediated by both NF-κB and AP-1 dependent pathways. COS could also suppress the phosphorylation of p38 MAPK and ERK1/2.
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116088
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Wei P,Xu QS,Du YG,et al. Chito-oligosaccharides Inhibit Production of Nitric Oxide in Lipopolysaccharide-Induced N9 Murine Microglial Cells[C]. 见:The 3rd Asian Communications of Glycobiology and Glycotechnology. 上海. 2011-10-27.
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