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学科主题生物化学
Chito-oligosaccharides Inhibit Production of Nitric Oxide in Lipopolysaccharide-Induced N9 Murine Microglial Cells
Wei P(魏鹏); Xu QS(许青松); Du YG(杜昱光); Yu C(于超)
会议文集Asian Communications of Glycobiology and Glycotechnology
会议名称The 3rd Asian Communications of Glycobiology and Glycotechnology
会议日期2011-10-27
2011
会议地点上海
其他题名壳寡糖在N9小胶质细胞中抑制由脂多糖诱导的一氧化氮的生成
页码72-0
出版者待补充
出版地待补充
合作性质墙报
部门归属1805
主办者中国科学院上海有机化学所
英文摘要Chito-oligosaccharides (COS) have been reported to exert many biological activities, such as antioxidant, antitumor and anti-inflammatory effects. However, the effects of COS on inflammatory microglial cells remain unknown. In present study, we examined the effect of COS on nitric oxide (NO) production in lipopolysaccharides (LPS) induced N9 microglial cells. Pretreatment with COS (50~200μg/ml) could markedly inhibit NO and inducible nitric oxide synthase (iNOS) production in LPS-induced N9 microglial cells. Signal transduction studies showed that COS remarkably inhibited LPS-induced phosphorylation of p38 mitogen-activated protein kinase (MAPK) and ERK1/2. Transcription factors NF-κB and AP-1 in N9 microglial cells were also significantly inhibited by pretreatment with COS for 24h. The anti-inflammatory effect of COS was comparable to that of positive control (hydrocortisone, 1M). In conclusion, our results suggest that the inhibitory effect of COS on the production of NO and iNOS in LPS-induced N9 microglial cells is mediated by both NF-κB and AP-1 dependent pathways. COS could also suppress the phosphorylation of p38 MAPK and ERK1/2.; Chito-oligosaccharides (COS) have been reported to exert many biological activities, such as antioxidant, antitumor and anti-inflammatory effects. However, the effects of COS on inflammatory microglial cells remain unknown. In present study, we examined the effect of COS on nitric oxide (NO) production in lipopolysaccharides (LPS) induced N9 microglial cells. Pretreatment with COS (50~200μg/ml) could markedly inhibit NO and inducible nitric oxide synthase (iNOS) production in LPS-induced N9 microglial cells. Signal transduction studies showed that COS remarkably inhibited LPS-induced phosphorylation of p38 mitogen-activated protein kinase (MAPK) and ERK1/2. Transcription factors NF-κB and AP-1 in N9 microglial cells were also significantly inhibited by pretreatment with COS for 24h. The anti-inflammatory effect of COS was comparable to that of positive control (hydrocortisone, 1M). In conclusion, our results suggest that the inhibitory effect of COS on the production of NO and iNOS in LPS-induced N9 microglial cells is mediated by both NF-κB and AP-1 dependent pathways. COS could also suppress the phosphorylation of p38 MAPK and ERK1/2.
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/116088
专题中国科学院大连化学物理研究所
通讯作者Du YG(杜昱光)
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GB/T 7714
Wei P,Xu QS,Du YG,et al. Chito-oligosaccharides Inhibit Production of Nitric Oxide in Lipopolysaccharide-Induced N9 Murine Microglial Cells[C]. 待补充:待补充,2011:72-0.
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