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学科主题: 物理化学
题名: Overloading study of basic compounds with a positively charged C18 column in liquid chromatography
作者: Wang, Chaoran1;  Guo, Zhimou1;  Long, Zhen1;  Zhang, Xiuli1;  Liang, Xinmiao1
通讯作者: 张秀莉 ;  梁鑫淼
关键词: Overloading ;  Basic compounds ;  Polar-copolymerize ;  Positive charge ;  RP-HPLC
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2013-03-15
DOI: 10.1016/j.chroma.2013.01.074
卷: 1281, 页:60-66
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: While tailing and overloading of basic compounds remain problematic on most RP columns, a new kind of positively charged RP column named XCharge C18 was found to be superior good for the separation of alkaloids in our practical use. In this work, the surface charge property of the XCharge C18 column was evaluated by the retention of NO3- under different pH values and buffer concentrations. A considerable and pH-dependent positive charge was confirmed on the column. Then overloading behaviors of bases were systematically studied using amitriptyline as a basic probe. Good peak shapes (Tf < 1.5) and extra high loadability with a C-0.5 of about 30,000 mg/L were observed on the column, with commonly used 0.1% formic acid as mobile phase additive. However, increasing the ionic strength of buffer with phosphates led to tailing peaks at high sample amount and sharp decline in loadability (C-0.5 of 2000-3000 mg/L), although it brought higher column efficiency at low sample amount. Higher pH also induced worse performance and lower loadability. The overall results demonstrated the importance of an appropriate level of ionic repulsion for the XCharge C18 column to achieve the good performance for bases, which could be explained by the multiple-site adsorption theory as ionic repulsion would shield the solute from occupying high-energy sites deeper in C18 layer. (c) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: BUFFERED MOBILE PHASES ;  REVERSED-PHASE ;  ADSORPTION MECHANISM ;  STATIONARY PHASES ;  IONIC-STRENGTH ;  IONIZABLE COMPOUNDS ;  PERFORMANCE ;  SEPARATION ;  RETENTION ;  BEHAVIOR
语种: 英语
WOS记录号: WOS:000315552700009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117541
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning Provin, Peoples R China

Recommended Citation:
Wang, Chaoran,Guo, Zhimou,Long, Zhen,et al. Overloading study of basic compounds with a positively charged C18 column in liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1281:60-66.
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