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学科主题物理化学
Overloading study of basic compounds with a positively charged C18 column in liquid chromatography
Wang, Chaoran; Guo, Zhimou; Long, Zhen; Zhang, Xiuli; Liang, Xinmiao; Zhang XL(张秀莉); Liang XM(梁鑫淼)
关键词Overloading Basic Compounds Polar-copolymerize Positive Charge Rp-hplc
刊名JOURNAL OF CHROMATOGRAPHY A
2013-03-15
DOI10.1016/j.chroma.2013.01.074
1281页:60-66
收录类别SCI
文章类型Article
部门归属18
项目归属1803
产权排名1,1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]BUFFERED MOBILE PHASES ; REVERSED-PHASE ; ADSORPTION MECHANISM ; STATIONARY PHASES ; IONIC-STRENGTH ; IONIZABLE COMPOUNDS ; PERFORMANCE ; SEPARATION ; RETENTION ; BEHAVIOR
英文摘要While tailing and overloading of basic compounds remain problematic on most RP columns, a new kind of positively charged RP column named XCharge C18 was found to be superior good for the separation of alkaloids in our practical use. In this work, the surface charge property of the XCharge C18 column was evaluated by the retention of NO3- under different pH values and buffer concentrations. A considerable and pH-dependent positive charge was confirmed on the column. Then overloading behaviors of bases were systematically studied using amitriptyline as a basic probe. Good peak shapes (Tf < 1.5) and extra high loadability with a C-0.5 of about 30,000 mg/L were observed on the column, with commonly used 0.1% formic acid as mobile phase additive. However, increasing the ionic strength of buffer with phosphates led to tailing peaks at high sample amount and sharp decline in loadability (C-0.5 of 2000-3000 mg/L), although it brought higher column efficiency at low sample amount. Higher pH also induced worse performance and lower loadability. The overall results demonstrated the importance of an appropriate level of ionic repulsion for the XCharge C18 column to achieve the good performance for bases, which could be explained by the multiple-site adsorption theory as ionic repulsion would shield the solute from occupying high-energy sites deeper in C18 layer. (c) 2013 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000315552700009
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/117541
专题中国科学院大连化学物理研究所
通讯作者Zhang XL(张秀莉); Liang XM(梁鑫淼)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning Provin, Peoples R China
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GB/T 7714
Wang, Chaoran,Guo, Zhimou,Long, Zhen,et al. Overloading study of basic compounds with a positively charged C18 column in liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1281:60-66.
APA Wang, Chaoran.,Guo, Zhimou.,Long, Zhen.,Zhang, Xiuli.,Liang, Xinmiao.,...&梁鑫淼.(2013).Overloading study of basic compounds with a positively charged C18 column in liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1281,60-66.
MLA Wang, Chaoran,et al."Overloading study of basic compounds with a positively charged C18 column in liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1281(2013):60-66.
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