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学科主题: 物理化学
题名: Cell Nucleus Targeting for Living Cell Extraction of Nucleic Acid Associated Proteins with Intracellular Nanoprobes of Magnetic Carbon Nanotubes
作者: Zhang, Yi1, 2;  Hu, Zhengyan1, 2;  Qin, Hongqiang1, 2;  Liu, Fangjie1, 2;  Cheng, Kai1, 2;  Wu, Ren'an1;  Zou, Hanfa1
通讯作者: 邹汉法 ;  吴仁安
刊名: ANALYTICAL CHEMISTRY
发表日期: 2013-08-06
DOI: 10.1021/ac401269g
卷: 85, 期:15, 页:7038-7043
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Since nanoparticles could be ingested by cells naturally and target at a specific cellular location as designed, the extraction of intracellular proteins from living cells for large-scale analysis by nanoprobes seems to be ideally possible. Nucleic acid associated proteins (NAaP) take the crucial position during biological processes in maintaining and regulating gene structure and gene related behaviors, yet there are still challenges during the global investigation of intracellular NAaP, especially from living cells. In this work, a strategy to extract intracellular proteins from living cells with the magnetic carbon nanotube (oMWCNT@Fe3O4) as an intracellular probe is developed, to achieve the high throughput analysis of NAaP from living human hepatoma BEL-7402 cells with a mass spectrometry-based proteomic approach. Due to the specific intracellular localization of the magnetic carbon nanotubes around nuclei and its strong interaction with nucleic acids, the highly efficient extraction was realized for cellular NAaP from living cells, with the capability of identifying 2383 intracellular NAaP from only ca. 10 000 living cells. This method exhibited potential applications in dynamic and in situ analysis of intracellular proteins.
关键词[WOS]: IN-VITRO ;  MASS-SPECTROMETRY ;  GENE-EXPRESSION ;  DNA ;  DELIVERY ;  PROTEOMICS ;  BINDING
语种: 英语
WOS记录号: WOS:000323014000012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117543
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Zhang, Yi,Hu, Zhengyan,Qin, Hongqiang,et al. Cell Nucleus Targeting for Living Cell Extraction of Nucleic Acid Associated Proteins with Intracellular Nanoprobes of Magnetic Carbon Nanotubes[J]. ANALYTICAL CHEMISTRY,2013,85(15):7038-7043.
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