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学科主题: 物理化学
题名: Mesenchymal Stem Cell and Chondrocyte Fates in a Multishear Microdevice Are Regulated by Yes-Associated Protein
作者: Zhong, Weiliang1;  Tian, Kang1;  Zheng, Xifu1;  Li, Linan1;  Zhang, Weiguo1;  Wang, Shouyu1;  Qin, Jianhua2
通讯作者: WeiguoZhang
刊名: STEM CELLS AND DEVELOPMENT
发表日期: 2013-07-01
DOI: 10.1089/scd.2012.0685
卷: 22, 期:14, 页:2083-2093
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 2,7
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Cell & Tissue Engineering ;  Hematology ;  Medicine, Research & Experimental ;  Transplantation
研究领域[WOS]: Cell Biology ;  Hematology ;  Research & Experimental Medicine ;  Transplantation
英文摘要: Mechanical cues exert considerable influence on the fates of stem cells and terminally differentiated chondrocytes. The elucidation of the interactions between cell fate and mechanical cues in nuclear mechanotransduction will provide new clues to modulate tissue homeostasis and regeneration. In this study, we used an integrated microfluidic perfusion device to simultaneously generate multiple-parameter fluid shear stresses to investigate the role of fluid flow stimuli in the regulation of Yes-associated protein (YAP) expression and the fates of mesenchymal stem cells (MSCs) and primary chondrocytes. YAP expression was regulated by the level of fluid flow stimulus in both MSCs and chondrocytes. An increase in the magnitude of stimulation enhanced the expression of YAP, ultimately resulting in an increase in osteogenesis and a decrease in adipogenesis for MSCs, and initiating dedifferentiation for chondrocytes. Cytochalasin D not only repressed nuclear YAP accumulation in the flow state, but also abrogated flow-induced effects on MSC differentiation and the chondrocyte phenotype, resulting in MSC adipogenesis and the maintenance of the chondrocyte phenotype. Our findings reveal the connection between YAP and MSC/chondrocyte fates in a fluid flow-induced mechanical microenvironment and provide new insights into the mechanisms by which mechanical cues regulate the fates of MSCs and chondrocytes.
关键词[WOS]: ORGAN SIZE CONTROL ;  SHEAR-STRESS ;  HIPPO PATHWAY ;  OSTEOGENIC DIFFERENTIATION ;  TISSUE REGENERATION ;  INTERSTITIAL FLOW ;  FOCAL ADHESION ;  GROWTH-CONTROL ;  SELF-RENEWAL ;  PROLIFERATION
语种: 英语
WOS记录号: WOS:000321186700010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117545
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Med Univ, Affiliated Hosp 1, Dept Orthopaed, Dalian 116011, Peoples R China
2.Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China

Recommended Citation:
Zhong, Weiliang,Tian, Kang,Zheng, Xifu,et al. Mesenchymal Stem Cell and Chondrocyte Fates in a Multishear Microdevice Are Regulated by Yes-Associated Protein[J]. STEM CELLS AND DEVELOPMENT,2013,22(14):2083-2093.
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