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学科主题: 物理化学
题名: Microfluidics study of intracellular calcium response to mechanical stimulation on single suspension cells
作者: Xu, Tao1, 2, 3;  Yue, Wanqing1;  Li, Cheuk-Wing1;  Yao, Xinsheng4;  Yang, Mengsu1, 2
通讯作者: MengsuYang
刊名: LAB ON A CHIP
发表日期: 2013
DOI: 10.1039/c3lc40880a
卷: 13, 期:6, 页:1060-1069
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 3,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Multidisciplinary ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Science & Technology - Other Topics
英文摘要: A microfluidic microdevice was developed to exert mechanical stimulation on an individual suspension cell for mechanosensation research. In this microfluidic chip, an individual cell was isolated from a population of cells, and trapped in a microchannel with a compressive component made of a deflectable membrane. The mechanosensation of HL60 cells ( leukemic cells) was studied using this chip, and the results showed that mechanical stimulations could trigger extracellular calcium to flow into HL60 cells through ion channels on cell membranes. The tension on individual HL60 cells exerted by the microdevice was showed large variations in the threshold of mechanosensation activation. In contrast to previous reports using patch clamp technique, there was little influence of cytoskeleton interruption on HL60 cell mechanosensation triggered by whole-cell compression. Additionally, two functional units were integrated in one chip for carrying out mechanosensation study in parallel, where HL60 cells ( leukemic cells) and Jurkat cells ( lymphocytes) were shown to respond to mechanical stimulation with different kinetics. The results demonstrated that the microfluidic device provides a novel approach to investigating the mechanosensation of single suspension cells in high-throughput.
关键词[WOS]: MECHANOSENSITIVE CHANNELS ;  CATION CHANNELS ;  LEUKEMIA-CELLS ;  MECHANOTRANSDUCTION ;  CYTOSKELETON ;  MEMBRANE ;  DEVICE ;  FORCE ;  LYSIS
语种: 英语
WOS记录号: WOS:000315166600010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117562
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.City Univ Hong Kong, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China
2.City Univ Hong Kong, Shenzhen Biotech & Hlth Ctr, Key Lab Biochip Technol, Shenzhen, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
4.Jinan Univ, Coll Pharm, Inst Tradit Chinese Med & Nat Prod, Guangzhou, Guangdong, Peoples R China

Recommended Citation:
Xu, Tao,Yue, Wanqing,Li, Cheuk-Wing,et al. Microfluidics study of intracellular calcium response to mechanical stimulation on single suspension cells[J]. LAB ON A CHIP,2013,13(6):1060-1069.
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