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学科主题: 物理化学
题名: Efficient purification of high-purity compounds from the stem of Lonicera japonica Thunb using two-dimensional preparative chromatography
作者: Jin, Hongli1, 2;  Liu, Yanfang1;  Feng, Jiatao1;  Guo, Zhimou1;  Wang, Chaoran1;  Zhong, Zhengsheng1;  Peng, Xiaojun2;  Dang, Jun3;  Tao, Yanduo3;  Liang, Xinmiao1
通讯作者: 梁鑫淼
关键词: High-purity compound ;  Lonicera japonica
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2013-08-01
DOI: 10.1002/jssc.201300319
卷: 36, 期:15, 页:2414-2420
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Purification of high-purity compounds from traditional Chinese medicines (TCMs) plays an important role in investigating their bioactivity. Nevertheless, it is often quite difficult to isolate compounds with high purity because of the complexity of TCMs in chemical composition. In this work, a two-dimensional preparation method was successfully developed for the preparation of high-purity compounds from the stem of Lonicera japonica Thunb, based on two novel polar copolymerized RP stationary phases, XAqua C3 and XAqua C18. An XAqua C3 prep column was used to separate the sample in the first-dimensional preparation, and 14 g of sample was fractionated into eight fractions with a recovery of 82%. An XAqua C18 prep column was selected to prepare high-purity compounds in the second-dimensional preparation for its good orthogonality with the XAqua C3 stationary phase. As a result, major compounds in the sample were isolated with more than 99% purity. This method is a potent method to realize the efficient purification of compounds with high purity from the stem of L. japonica Thunb and it shows great potential in the separation of high-purity compounds from complex samples.
关键词[WOS]: PHASE LIQUID-CHROMATOGRAPHY ;  STATIONARY PHASES ;  IN-VITRO ;  SEPARATION ;  OPTIMIZATION ;  PERFORMANCE ;  COLUMN ;  C18
语种: 英语
WOS记录号: WOS:000322709900005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117575
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China
2.Dalian Univ Technol, Fac Chem Environm & Biol Sci & Technol, State Key Lab Fine Chem, Dalian, Peoples R China
3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Peoples R China

Recommended Citation:
Jin, Hongli,Liu, Yanfang,Feng, Jiatao,et al. Efficient purification of high-purity compounds from the stem of Lonicera japonica Thunb using two-dimensional preparative chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(15):2414-2420.
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