DICP OpenIR
Subject Area物理化学
Efficient purification of high-purity compounds from the stem of Lonicera japonica Thunb using two-dimensional preparative chromatography
Jin, Hongli1,2; Liu, Yanfang1; Feng, Jiatao1; Guo, Zhimou1; Wang, Chaoran1; Zhong, Zhengsheng1; Peng, Xiaojun2; Dang, Jun3; Tao, Yanduo3; Liang, Xinmiao1; Liang XM(梁鑫淼)
KeywordHigh-purity Compound Lonicera Japonica
Source PublicationJOURNAL OF SEPARATION SCIENCE
2013-08-01
DOI10.1002/jssc.201300319
Volume36Issue:15Pages:2414-2420
Indexed BySCI
SubtypeArticle
Department18
Funding Project1803
Contribution Rank1,1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordPHASE LIQUID-CHROMATOGRAPHY ; STATIONARY PHASES ; IN-VITRO ; SEPARATION ; OPTIMIZATION ; PERFORMANCE ; COLUMN ; C18
AbstractPurification of high-purity compounds from traditional Chinese medicines (TCMs) plays an important role in investigating their bioactivity. Nevertheless, it is often quite difficult to isolate compounds with high purity because of the complexity of TCMs in chemical composition. In this work, a two-dimensional preparation method was successfully developed for the preparation of high-purity compounds from the stem of Lonicera japonica Thunb, based on two novel polar copolymerized RP stationary phases, XAqua C3 and XAqua C18. An XAqua C3 prep column was used to separate the sample in the first-dimensional preparation, and 14 g of sample was fractionated into eight fractions with a recovery of 82%. An XAqua C18 prep column was selected to prepare high-purity compounds in the second-dimensional preparation for its good orthogonality with the XAqua C3 stationary phase. As a result, major compounds in the sample were isolated with more than 99% purity. This method is a potent method to realize the efficient purification of compounds with high purity from the stem of L. japonica Thunb and it shows great potential in the separation of high-purity compounds from complex samples.
Language英语
WOS IDWOS:000322709900005
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/117575
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiang XM(梁鑫淼)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China
2.Dalian Univ Technol, Fac Chem Environm & Biol Sci & Technol, State Key Lab Fine Chem, Dalian, Peoples R China
3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Peoples R China
Recommended Citation
GB/T 7714
Jin, Hongli,Liu, Yanfang,Feng, Jiatao,et al. Efficient purification of high-purity compounds from the stem of Lonicera japonica Thunb using two-dimensional preparative chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(15):2414-2420.
APA Jin, Hongli.,Liu, Yanfang.,Feng, Jiatao.,Guo, Zhimou.,Wang, Chaoran.,...&梁鑫淼.(2013).Efficient purification of high-purity compounds from the stem of Lonicera japonica Thunb using two-dimensional preparative chromatography.JOURNAL OF SEPARATION SCIENCE,36(15),2414-2420.
MLA Jin, Hongli,et al."Efficient purification of high-purity compounds from the stem of Lonicera japonica Thunb using two-dimensional preparative chromatography".JOURNAL OF SEPARATION SCIENCE 36.15(2013):2414-2420.
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