DICP OpenIR
Subject Area物理化学
A novel ionic-bonded cellulose stationary phase for saccharide separation
Sheng, Qianying1; Ke, Yanxiong1; Li, Kuiyong1; Yu, Dongping2; Liang, Xinmiao1,2; Ke YX(柯燕雄); Liang XM(梁鑫淼)
KeywordCellulose-coated Stationary Phase Ion interactIon Saccharide Separation Hydrophilic Interaction Liquid Chromatography High-performance Liquid Chromatography Maldi-qit-tof-ms
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2013-05-24
DOI10.1016/j.chroma.2013.03.045
Volume1291Pages:56-63
Indexed BySCI
SubtypeArticle
Department18
Funding Project1803
Contribution Rank2,4
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordHYDROPHILIC-INTERACTION CHROMATOGRAPHY ; INTERACTION LIQUID-CHROMATOGRAPHY ; ANION-EXCHANGE CHROMATOGRAPHY ; MASS-SPECTROMETRY ; POLAR COMPOUNDS ; REVERSED-PHASE ; PEPTIDES ; OLIGOSACCHARIDE ; ARRAYS
AbstractA hydrophilic interaction liquid chromatography (HILIC) stationary phase of cellulose-coated silica was synthesized as a novel saccharide separation material. The material, prepared with a method based on ionic interaction, was demonstrated to be efficient for immobilization of saccharides on silica supports. The method is more efficient than traditional immobilized saccharide stationary phase synthesis methods. It was evolved from a method using anion exchanger microparticles agglomerated onto macroparticles of cation exchangers to produce anion exchangers. Cationic cellulose, which has a large number of hydroxyl groups, was immobilized on sulfonated silica. The cellulose-coated stationary phase we designed used strong hydrogen bonding between cellulose hydroxyl and carbohydrate compounds for HILIC retention and separation. The stationary phase was successfully used to separate the samples of polar compounds and the complex samples of oligosaccharides, and demonstrated good reproducibility and stability. The material exhibited good separation selectivity for carbohydrates and ability to enrich glycosylated peptides. The method described here is easy to achieve, environmentally safe and innovative than other methods. It also has extensive application possibilities to separate other categories polar compounds. (C) 2013 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000318745400008
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/117578
Collection中国科学院大连化学物理研究所
Corresponding AuthorKe YX(柯燕雄); Liang XM(梁鑫淼)
Affiliation1.E China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Sheng, Qianying,Ke, Yanxiong,Li, Kuiyong,et al. A novel ionic-bonded cellulose stationary phase for saccharide separation[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1291:56-63.
APA Sheng, Qianying.,Ke, Yanxiong.,Li, Kuiyong.,Yu, Dongping.,Liang, Xinmiao.,...&梁鑫淼.(2013).A novel ionic-bonded cellulose stationary phase for saccharide separation.JOURNAL OF CHROMATOGRAPHY A,1291,56-63.
MLA Sheng, Qianying,et al."A novel ionic-bonded cellulose stationary phase for saccharide separation".JOURNAL OF CHROMATOGRAPHY A 1291(2013):56-63.
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