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学科主题: 物理化学
题名: A novel ionic-bonded cellulose stationary phase for saccharide separation
作者: Sheng, Qianying1;  Ke, Yanxiong1;  Li, Kuiyong1;  Yu, Dongping2;  Liang, Xinmiao1, 2
通讯作者: 柯燕雄 ;  梁鑫淼
关键词: Cellulose-coated stationary phase ;  Ion interaction ;  Saccharide separation ;  Hydrophilic interaction liquid chromatography ;  High-performance liquid chromatography ;  MALDI-QIT-TOF-MS
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2013-05-24
DOI: 10.1016/j.chroma.2013.03.045
卷: 1291, 页:56-63
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 2,4
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A hydrophilic interaction liquid chromatography (HILIC) stationary phase of cellulose-coated silica was synthesized as a novel saccharide separation material. The material, prepared with a method based on ionic interaction, was demonstrated to be efficient for immobilization of saccharides on silica supports. The method is more efficient than traditional immobilized saccharide stationary phase synthesis methods. It was evolved from a method using anion exchanger microparticles agglomerated onto macroparticles of cation exchangers to produce anion exchangers. Cationic cellulose, which has a large number of hydroxyl groups, was immobilized on sulfonated silica. The cellulose-coated stationary phase we designed used strong hydrogen bonding between cellulose hydroxyl and carbohydrate compounds for HILIC retention and separation. The stationary phase was successfully used to separate the samples of polar compounds and the complex samples of oligosaccharides, and demonstrated good reproducibility and stability. The material exhibited good separation selectivity for carbohydrates and ability to enrich glycosylated peptides. The method described here is easy to achieve, environmentally safe and innovative than other methods. It also has extensive application possibilities to separate other categories polar compounds. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: HYDROPHILIC-INTERACTION CHROMATOGRAPHY ;  INTERACTION LIQUID-CHROMATOGRAPHY ;  ANION-EXCHANGE CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  POLAR COMPOUNDS ;  REVERSED-PHASE ;  PEPTIDES ;  OLIGOSACCHARIDE ;  ARRAYS
语种: 英语
WOS记录号: WOS:000318745400008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117578
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.E China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Sheng, Qianying,Ke, Yanxiong,Li, Kuiyong,et al. A novel ionic-bonded cellulose stationary phase for saccharide separation[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1291:56-63.
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