DICP OpenIR
Subject Area物理化学
Development and evaluation of new imidazolium-based zwitterionic stationary phases for hydrophilic interaction chromatography
Qiao, Lizhen; Dou, Abo; Shi, Xianzhe; Li, Hua; Shan, Yuanhong; Lu, Xin; Xu, Guowang; Shi XZ(石先哲); Xu GW(许国旺)
KeywordImidazolium Zwitterionic Stationary Phase Hydrophilic Interaction Chromatography Core-shell Ionic Liquid
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2013-04-19
DOI10.1016/j.chroma.2013.02.066
Volume1286Pages:137-145
Indexed BySCI ; ISTP
SubtypeArticle
Department18
Funding Project1808
Contribution Rank1,1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordINTERACTION LIQUID-CHROMATOGRAPHY ; CONFINED IONIC LIQUID ; RETENTION BEHAVIOR ; GAS-CHROMATOGRAPHY ; REVERSED-PHASE ; MOBILE-PHASE ; URIC-ACID ; MASS-SPECTROMETRY ; POLAR COMPOUNDS ; SEPARATION
AbstractHydrophilic interaction liquid chromatography (HILIC) has been widely used for separating polar compounds as a complement mode to reversed-phase liquid chromatography. The development of new stationary phases for HILIC is significant to improve the coverage of various polar and hydrophilic compounds. The present study described the preparation and application of novel imidazolium-based zwitterionic stationary phases. 1-Vinyl-3-(butyl-4-sulfonate) imidazolium was synthesized from 1-vinylimidazole and 1,4-butane sultone, then bonded to the surface of 3-mercaptopropyl modified silica particles (core-shell silica and totally porous silica) by "thiol-ene" click chemistry to obtain the resulting zwitterionic stationary phase with a positively charged imidazole ring and a negatively charged sulfonate group. The zwitterionic stationary phases exhibited good selectivity and favorable retention for a wide range of polar solutes (nucleosides, nucleic acid bases, benzoic organic acids, uric acid and its methyl derivatives, water-soluble vitamins) as compared to a bare silica column. The column efficiency could reach up to 100,000 theoretical plates/m with cytosine as the test solute. The retention changes of various types of test solutes were investigated under different chromatographic conditions including water content, pH, buffer salt concentration in mobile phase and column temperature. The results indicated that the retention of solutes on the stationary phase was the outcome of a mixed-mode retention mechanism (i.e. a combination of adsorptive and partitioning interactions). In conclusion, the new imidazolium-based zwitterionic stationary phases have shown excellent chromatographic behavior for a variety of polar solutes under HILIC mode, and have a great potential as a new type of stationary phases for HILIC. (c) 2013 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000317156100016
Citation statistics
Cited Times:55[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/117595
Collection中国科学院大连化学物理研究所
Corresponding AuthorShi XZ(石先哲); Xu GW(许国旺)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Qiao, Lizhen,Dou, Abo,Shi, Xianzhe,et al. Development and evaluation of new imidazolium-based zwitterionic stationary phases for hydrophilic interaction chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1286:137-145.
APA Qiao, Lizhen.,Dou, Abo.,Shi, Xianzhe.,Li, Hua.,Shan, Yuanhong.,...&许国旺.(2013).Development and evaluation of new imidazolium-based zwitterionic stationary phases for hydrophilic interaction chromatography.JOURNAL OF CHROMATOGRAPHY A,1286,137-145.
MLA Qiao, Lizhen,et al."Development and evaluation of new imidazolium-based zwitterionic stationary phases for hydrophilic interaction chromatography".JOURNAL OF CHROMATOGRAPHY A 1286(2013):137-145.
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