DICP OpenIR
Subject Area物理化学
In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer
Li, Kai1,2; Sun, Zhen3; Zheng, Jianyong1,2; Lu, Yuanyuan1,2; Bian, Yangyang3; Ye, Mingliang3; Wang, Xin1,2; Nie, Yongzhan1,2; Zou, Hanfa3; Fan, Daiming1,2; Zou HF(邹汉法); DaimingFan
KeywordBiomarker Cell Surface Capturing Gastric Cancer Glycoproteomics Multidrug Resistance
Source PublicationJOURNAL OF PROTEOMICS
2013-04-26
ISSN1874-3919
DOI10.1016/j.jprot.2013.02.021
Volume82Pages:130-140
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank2,2
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordN-LINKED GLYCOSYLATION ; QUANTITATIVE PROTEOMICS ; OVARIAN-CARCINOMA ; DRUG-RESISTANCE ; P-GLYCOPROTEIN ; EXPRESSION ; IDENTIFICATION ; SERUM ; LINES ; QUANTIFICATION
AbstractHuman gastric cancer is a big public health problem. Multidrug resistance is a main obstacle to successful chemotherapeutic treatment in gastric cancers and the underlying mechanism is not clear. Glycosylation, one of the most important post translational modifications of proteins, plays a vital role in diverse aspects of tumor progression. In the present study, we applied two multidrug resistance cell lines and their parental drug sensitive gastric cancer cell line to a modified cell surface capturing strategy with triplex labeling to characterize MDR related cell surface glycoproteome. Finally, 56 cell membrane glycoproteins were successfully identified via combination of identification by glycopeptides and quantitation by non-glycopeptides, and 11 of them were found to be differentially expressed with the same trend in both drug resistant cell lines compared with that in sensitive cell line. The further analysis by western blot and in vitro drug sensitivity assay demonstrated that our approach is reliable and accurate and suggested that these glycoproteins may represent as biomarkers for multidrug resistance in gastric cancer.
Language英语
WOS IDWOS:000319643300010
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/117604
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法); DaimingFan
Affiliation1.Fourth Mil Med Univ, State Key Lab Canc Biol, Xian 710032, Shaanxi Provinc, Peoples R China
2.Fourth Mil Med Univ, Xijing Hosp Digest Dis, Xian 710032, Shaanxi Provinc, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Li, Kai,Sun, Zhen,Zheng, Jianyong,et al. In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer[J]. JOURNAL OF PROTEOMICS,2013,82:130-140.
APA Li, Kai.,Sun, Zhen.,Zheng, Jianyong.,Lu, Yuanyuan.,Bian, Yangyang.,...&DaimingFan.(2013).In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer.JOURNAL OF PROTEOMICS,82,130-140.
MLA Li, Kai,et al."In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer".JOURNAL OF PROTEOMICS 82(2013):130-140.
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