DICP OpenIR
学科主题物理化学
In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer
Li, Kai1,2; Sun, Zhen3; Zheng, Jianyong1,2; Lu, Yuanyuan1,2; Bian, Yangyang3; Ye, Mingliang3; Wang, Xin1,2; Nie, Yongzhan1,2; Zou, Hanfa3; Fan, Daiming1,2; Zou HF(邹汉法); DaimingFan
关键词Biomarker Cell Surface Capturing Gastric Cancer Glycoproteomics Multidrug Resistance
刊名JOURNAL OF PROTEOMICS
2013-04-26
ISSN1874-3919
DOI10.1016/j.jprot.2013.02.021
82页:130-140
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名2,2
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]N-LINKED GLYCOSYLATION ; QUANTITATIVE PROTEOMICS ; OVARIAN-CARCINOMA ; DRUG-RESISTANCE ; P-GLYCOPROTEIN ; EXPRESSION ; IDENTIFICATION ; SERUM ; LINES ; QUANTIFICATION
英文摘要Human gastric cancer is a big public health problem. Multidrug resistance is a main obstacle to successful chemotherapeutic treatment in gastric cancers and the underlying mechanism is not clear. Glycosylation, one of the most important post translational modifications of proteins, plays a vital role in diverse aspects of tumor progression. In the present study, we applied two multidrug resistance cell lines and their parental drug sensitive gastric cancer cell line to a modified cell surface capturing strategy with triplex labeling to characterize MDR related cell surface glycoproteome. Finally, 56 cell membrane glycoproteins were successfully identified via combination of identification by glycopeptides and quantitation by non-glycopeptides, and 11 of them were found to be differentially expressed with the same trend in both drug resistant cell lines compared with that in sensitive cell line. The further analysis by western blot and in vitro drug sensitivity assay demonstrated that our approach is reliable and accurate and suggested that these glycoproteins may represent as biomarkers for multidrug resistance in gastric cancer.
语种英语
WOS记录号WOS:000319643300010
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/117604
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); DaimingFan
作者单位1.Fourth Mil Med Univ, State Key Lab Canc Biol, Xian 710032, Shaanxi Provinc, Peoples R China
2.Fourth Mil Med Univ, Xijing Hosp Digest Dis, Xian 710032, Shaanxi Provinc, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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Li, Kai,Sun, Zhen,Zheng, Jianyong,et al. In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer[J]. JOURNAL OF PROTEOMICS,2013,82:130-140.
APA Li, Kai.,Sun, Zhen.,Zheng, Jianyong.,Lu, Yuanyuan.,Bian, Yangyang.,...&DaimingFan.(2013).In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer.JOURNAL OF PROTEOMICS,82,130-140.
MLA Li, Kai,et al."In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer".JOURNAL OF PROTEOMICS 82(2013):130-140.
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