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学科主题: 物理化学
题名: In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer
作者: Li, Kai1, 2;  Sun, Zhen3;  Zheng, Jianyong1, 2;  Lu, Yuanyuan1, 2;  Bian, Yangyang3;  Ye, Mingliang3;  Wang, Xin1, 2;  Nie, Yongzhan1, 2;  Zou, Hanfa3;  Fan, Daiming1, 2
通讯作者: 邹汉法 ;  DaimingFan
关键词: Biomarker ;  Cell surface capturing ;  Gastric cancer ;  Glycoproteomics ;  Multidrug resistance
刊名: JOURNAL OF PROTEOMICS
发表日期: 2013-04-26
DOI: 10.1016/j.jprot.2013.02.021
卷: 82, 页:130-140
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 2,2
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Human gastric cancer is a big public health problem. Multidrug resistance is a main obstacle to successful chemotherapeutic treatment in gastric cancers and the underlying mechanism is not clear. Glycosylation, one of the most important post translational modifications of proteins, plays a vital role in diverse aspects of tumor progression. In the present study, we applied two multidrug resistance cell lines and their parental drug sensitive gastric cancer cell line to a modified cell surface capturing strategy with triplex labeling to characterize MDR related cell surface glycoproteome. Finally, 56 cell membrane glycoproteins were successfully identified via combination of identification by glycopeptides and quantitation by non-glycopeptides, and 11 of them were found to be differentially expressed with the same trend in both drug resistant cell lines compared with that in sensitive cell line. The further analysis by western blot and in vitro drug sensitivity assay demonstrated that our approach is reliable and accurate and suggested that these glycoproteins may represent as biomarkers for multidrug resistance in gastric cancer.
关键词[WOS]: N-LINKED GLYCOSYLATION ;  QUANTITATIVE PROTEOMICS ;  OVARIAN-CARCINOMA ;  DRUG-RESISTANCE ;  P-GLYCOPROTEIN ;  EXPRESSION ;  IDENTIFICATION ;  SERUM ;  LINES ;  QUANTIFICATION
语种: 英语
WOS记录号: WOS:000319643300010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117604
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Fourth Mil Med Univ, State Key Lab Canc Biol, Xian 710032, Shaanxi Provinc, Peoples R China
2.Fourth Mil Med Univ, Xijing Hosp Digest Dis, Xian 710032, Shaanxi Provinc, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Li, Kai,Sun, Zhen,Zheng, Jianyong,et al. In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer[J]. JOURNAL OF PROTEOMICS,2013,82:130-140.
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