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学科主题: 物理化学
题名: Encapsulated human hepatocellular carcinoma cells by alginate gel beads as an in vitro metastasis model
作者: Xu, Xiao-xi1, 2;  Liu, Chang1, 2;  Liu, Yang1;  Li, Nan1, 2;  Guo, Xin1;  Wang, Shu-jun1, 3;  Sun, Guang-wei1;  Wang, Wei1;  Ma, Xiao-jun1
通讯作者: 孙广炜 ;  马小军
关键词: Alginate bead ;  Metastasis model ;  Tumor microenvironment ;  Matrix metalloproteinase ;  Invasion
刊名: EXPERIMENTAL CELL RESEARCH
发表日期: 2013-08-15
DOI: 10.1016/j.yexcr.2013.05.013
卷: 319, 期:14, 页:2135-2144
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Oncology ;  Cell Biology
研究领域[WOS]: Oncology ;  Cell Biology
英文摘要: Hepatocellular carcinoma (HCC) is the most common primary liver cancer and often forms metastases, which are the most important prognostic factors. For further elucidation of the mechanism underlying the progression and metastasis of HCC, a culture system mimicking the in vivo tumor microenvironment is needed. In this study, we investigated the metastatic ability of HCC cells cultured within alginate gel (ALG) beads. In the culture system, HCC cells formed spheroids by proliferation and maintained in nuclear abnormalities. The gene and protein expression of metastasis-related molecules was increased in ALG beads, compared with the traditional adhesion culture. Furthermore, several gene expression levels in ALG bead culture system were even closer to liver cancer tissues. More importantly, in vitro invasion assay showed that the invasion cells derived from ALG beads was 7.8-fold higher than adhesion cells. Our results indicated that the in vitro three-dimensional (3D) model based on ALG beads increased metastatic ability compared with adhesion culture, even partly mimicked the in vivo tumor tissues. Moreover, due to the controllable preparation conditions, steady characteristics and production at large-scale, the 3D ALG bead model would become an important tool used in the high-throughput screening of anti-metastasis drugs and the metastatic mechanism research. (C) 2013 Elsevier Inc. All rights reserved.
关键词[WOS]: MATRIX METALLOPROTEINASES ;  MESENCHYMAL TRANSITION ;  BIOARTIFICIAL PANCREAS ;  TUMOR SPHEROIDS ;  CULTURE-SYSTEM ;  HEPG2 CELLS ;  CANCER ;  INVASION ;  TISSUE ;  3D
语种: 英语
WOS记录号: WOS:000322804900002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117606
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China

Recommended Citation:
Xu, Xiao-xi,Liu, Chang,Liu, Yang,et al. Encapsulated human hepatocellular carcinoma cells by alginate gel beads as an in vitro metastasis model[J]. EXPERIMENTAL CELL RESEARCH,2013,319(14):2135-2144.
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