DICP OpenIR
Subject Area物理化学
Encapsulated human hepatocellular carcinoma cells by alginate gel beads as an in vitro metastasis model
Xu, Xiao-xi1,2; Liu, Chang1,2; Liu, Yang1; Li, Nan1,2; Guo, Xin1; Wang, Shu-jun1,3; Sun, Guang-wei1; Wang, Wei1; Ma, Xiao-jun1; Sun GW(孙广炜); Ma XJ(马小军)
KeywordAlginate Bead Metastasis Model Tumor Microenvironment Matrix Metalloproteinase Invasion
Source PublicationEXPERIMENTAL CELL RESEARCH
2013-08-15
DOI10.1016/j.yexcr.2013.05.013
Volume319Issue:14Pages:2135-2144
Indexed BySCI
SubtypeArticle
Department18
Funding Project1802
Contribution Rank1,1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectOncology ; Cell Biology
WOS Research AreaOncology ; Cell Biology
WOS KeywordMATRIX METALLOPROTEINASES ; MESENCHYMAL TRANSITION ; BIOARTIFICIAL PANCREAS ; TUMOR SPHEROIDS ; CULTURE-SYSTEM ; HEPG2 CELLS ; CANCER ; INVASION ; TISSUE ; 3D
AbstractHepatocellular carcinoma (HCC) is the most common primary liver cancer and often forms metastases, which are the most important prognostic factors. For further elucidation of the mechanism underlying the progression and metastasis of HCC, a culture system mimicking the in vivo tumor microenvironment is needed. In this study, we investigated the metastatic ability of HCC cells cultured within alginate gel (ALG) beads. In the culture system, HCC cells formed spheroids by proliferation and maintained in nuclear abnormalities. The gene and protein expression of metastasis-related molecules was increased in ALG beads, compared with the traditional adhesion culture. Furthermore, several gene expression levels in ALG bead culture system were even closer to liver cancer tissues. More importantly, in vitro invasion assay showed that the invasion cells derived from ALG beads was 7.8-fold higher than adhesion cells. Our results indicated that the in vitro three-dimensional (3D) model based on ALG beads increased metastatic ability compared with adhesion culture, even partly mimicked the in vivo tumor tissues. Moreover, due to the controllable preparation conditions, steady characteristics and production at large-scale, the 3D ALG bead model would become an important tool used in the high-throughput screening of anti-metastasis drugs and the metastatic mechanism research. (C) 2013 Elsevier Inc. All rights reserved.
Language英语
WOS IDWOS:000322804900002
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/117606
Collection中国科学院大连化学物理研究所
Corresponding AuthorSun GW(孙广炜); Ma XJ(马小军)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China
Recommended Citation
GB/T 7714
Xu, Xiao-xi,Liu, Chang,Liu, Yang,et al. Encapsulated human hepatocellular carcinoma cells by alginate gel beads as an in vitro metastasis model[J]. EXPERIMENTAL CELL RESEARCH,2013,319(14):2135-2144.
APA Xu, Xiao-xi.,Liu, Chang.,Liu, Yang.,Li, Nan.,Guo, Xin.,...&马小军.(2013).Encapsulated human hepatocellular carcinoma cells by alginate gel beads as an in vitro metastasis model.EXPERIMENTAL CELL RESEARCH,319(14),2135-2144.
MLA Xu, Xiao-xi,et al."Encapsulated human hepatocellular carcinoma cells by alginate gel beads as an in vitro metastasis model".EXPERIMENTAL CELL RESEARCH 319.14(2013):2135-2144.
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