中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Analysis of human serum phosphopeptidome by a focused database searching strategy
作者: Zhu, Jun1;  Wang, Fangjun1;  Cheng, Kai1;  Song, Chunxia1;  Qin, Hongqiang1;  Hu, Lianghai2;  Figeys, Daniel3, 4;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 叶明亮 ;  邹汉法
关键词: Human serum ;  Phosphopeptidome ;  Ti (IV)-MCM-41 ;  Focused database
刊名: JOURNAL OF PROTEOMICS
发表日期: 2013-01-14
DOI: 10.1016/j.jprot.2012.10.006
卷: 78, 页:389-397
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: As human serum is an important source for early diagnosis of many serious diseases, analysis of serum proteome and peptidome has been extensively performed. However, the serum phosphopeptidome was less explored probably because the effective method for database searching is lacking. Conventional database searching strategy always uses the whole proteome database, which is very time-consuming for phosphopeptidome search due to the huge searching space resulted from the high redundancy of the database and the setting of dynamic modifications during searching. In this work, a focused database searching strategy using an in-house collected human serum pro-peptidome target/decoy database (HuSPep) was established. It was found that the searching time was significantly decreased without compromising the identification sensitivity. By combining size-selective Ti (IV)-MCM-41 enrichment, RP-RP off-line separation, and complementary CID and ETD fragmentation with the new searching strategy, 143 unique endogenous phosphopeptides and 133 phosphorylation sites (109 novel sites) were identified from human serum with high reliability. (C) 2012 Elsevier B.V. All rights reserved.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  CAPILLARY TRAP COLUMN ;  RP-RP-HPLC ;  PHOSPHOPROTEOME ANALYSIS ;  BIOMARKER DISCOVERY ;  ENRICHMENT ;  PEPTIDOME ;  IDENTIFICATION ;  EXTRACTION ;  SEPARATION
语种: 英语
WOS记录号: WOS:000316706800030
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117609
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2013iX0rUTKa2t.PDF(731KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Jilin Univ, Coll Life Sci, Changchun 130023, Peoples R China
3.Fac Med, Dept Biochem Microbiol & Immunol, Ottawa Inst Syst Biol, Ottawa, ON, Canada
4.Univ Ottawa, Dept Chem, Ottawa, ON K1N 6N5, Canada

Recommended Citation:
Zhu, Jun,Wang, Fangjun,Cheng, Kai,et al. Analysis of human serum phosphopeptidome by a focused database searching strategy[J]. JOURNAL OF PROTEOMICS,2013,78:389-397.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Zhu, Jun]'s Articles
 [Wang, Fangjun]'s Articles
 [Cheng, Kai]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Zhu, Jun]‘s Articles
 [Wang, Fangjun]‘s Articles
 [Cheng, Kai]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2013iX0rUTKa2t.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace