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学科主题: 物理化学
题名: A simple integrated system for rapid analysis of sialic-acid-containing N-glycopeptides from human serum
作者: Zhu, Jun1;  Wang, Fangjun1;  Cheng, Kai1;  Dong, Jing1;  Sun, Deguang2;  Chen, Rui1;  Wang, Liming2;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 邹汉法
关键词: ACN-improved digestion ;  Filter-aided sample preparation ;  Glycoproteomics ;  Human serum ;  Sialic acid-containing N-glycopeptide ;  Ti(IV)-IMAC
刊名: PROTEOMICS
发表日期: 2013-04-01
DOI: 10.1002/pmic.201200367
卷: 13, 期:8, 页:1306-1313
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Terminal sialylation is very important in cancer biology and has been extensively investigated for the discovery of potential clinical biomarkers of cancers. In this study, we presented a novel approach, by using of Ti(IV)-IMAC, to enrich sialic-acid-containing N-glycopeptides for the analysis of terminal sialylation. Compared with conventional method using TiO2, this approach obtained 2.5 times more glycopeptides and glycosylation sites. Then, a simple integrated system combining filter-aided sample preparation, ACN-improved digestion, and Ti(IV)-IMAC enrichment was established for efficient analysis. In this system, protein digestion, glycopeptide enrichment, and deglycosylation were integrated and were performed sequentially in a single filter unit without any need for desalting, lyophilization, or sample transfer procedures. As a result, the number of identifications was improved by 1.5-fold and the total processing time was drastically reduced to only 78 h. By using this system, fast and efficient analysis of human serum sialylated N-glycoproteome was achieved. From only 1 L of human serum, 217 unique glycopeptides and 194 glycosylation sites were successfully identified.
关键词[WOS]: MASS-SPECTROMETRY ;  PROTEOME ANALYSIS ;  ABERRANT GLYCOSYLATION ;  ENRICHMENT ;  CHROMATOGRAPHY ;  IDENTIFICATION ;  SOLUBILIZATION ;  PROTEINS ;  PLASMA
语种: 英语
WOS记录号: WOS:000317684800008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117616
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian, Peoples R China

Recommended Citation:
Zhu, Jun,Wang, Fangjun,Cheng, Kai,et al. A simple integrated system for rapid analysis of sialic-acid-containing N-glycopeptides from human serum[J]. PROTEOMICS,2013,13(8):1306-1313.
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