DICP OpenIR
Subject Area物理化学
A simple integrated system for rapid analysis of sialic-acid-containing N-glycopeptides from human serum
Zhu, Jun1; Wang, Fangjun1; Cheng, Kai1; Dong, Jing1; Sun, Deguang2; Chen, Rui1; Wang, Liming2; Ye, Mingliang1; Zou, Hanfa1; Zou HF(邹汉法)
KeywordAcn-improved Digestion Filter-aided Sample Preparation Glycoproteomics Human Serum Sialic Acid-containing N-glycopeptide Ti(Iv)-imac
Source PublicationPROTEOMICS
2013-04-01
ISSN1615-9853
DOI10.1002/pmic.201200367
Volume13Issue:8Pages:1306-1313
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1,1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordMASS-SPECTROMETRY ; PROTEOME ANALYSIS ; ABERRANT GLYCOSYLATION ; ENRICHMENT ; CHROMATOGRAPHY ; IDENTIFICATION ; SOLUBILIZATION ; PROTEINS ; PLASMA
AbstractTerminal sialylation is very important in cancer biology and has been extensively investigated for the discovery of potential clinical biomarkers of cancers. In this study, we presented a novel approach, by using of Ti(IV)-IMAC, to enrich sialic-acid-containing N-glycopeptides for the analysis of terminal sialylation. Compared with conventional method using TiO2, this approach obtained 2.5 times more glycopeptides and glycosylation sites. Then, a simple integrated system combining filter-aided sample preparation, ACN-improved digestion, and Ti(IV)-IMAC enrichment was established for efficient analysis. In this system, protein digestion, glycopeptide enrichment, and deglycosylation were integrated and were performed sequentially in a single filter unit without any need for desalting, lyophilization, or sample transfer procedures. As a result, the number of identifications was improved by 1.5-fold and the total processing time was drastically reduced to only 78 h. By using this system, fast and efficient analysis of human serum sialylated N-glycoproteome was achieved. From only 1 L of human serum, 217 unique glycopeptides and 194 glycosylation sites were successfully identified.
Language英语
WOS IDWOS:000317684800008
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/117616
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Jun,Wang, Fangjun,Cheng, Kai,et al. A simple integrated system for rapid analysis of sialic-acid-containing N-glycopeptides from human serum[J]. PROTEOMICS,2013,13(8):1306-1313.
APA Zhu, Jun.,Wang, Fangjun.,Cheng, Kai.,Dong, Jing.,Sun, Deguang.,...&邹汉法.(2013).A simple integrated system for rapid analysis of sialic-acid-containing N-glycopeptides from human serum.PROTEOMICS,13(8),1306-1313.
MLA Zhu, Jun,et al."A simple integrated system for rapid analysis of sialic-acid-containing N-glycopeptides from human serum".PROTEOMICS 13.8(2013):1306-1313.
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