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学科主题: 物理化学
题名: Microcapsule Co-Culture System Enhances Neural Differentiation of Mesenchymal Stem Cells
作者: Liu, Yang1;  Liu, Chang1, 2;  Nan, Feng3;  Xiao, Jing4;  Lian, Jian-chun5;  He, Xin5;  Guo, Xin1;  Sun, Guang-wei1;  Ma, Xiao-jun1
通讯作者: 孙广炜 ;  马小军
关键词: Cell co-culture ;  Mesenchymal stem cell ;  Microcapsule ;  Neural differentiation
刊名: JOURNAL OF HARD TISSUE BIOLOGY
发表日期: 2013-04-01
卷: 22, 期:2, 页:241-247
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 1,1
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Engineering, Biomedical
研究领域[WOS]: Engineering
英文摘要: Cell co-culture plays a key role in inducing the committed differentiation of stem cells in vitro, which mimics the specific tissue microenvironment in vivo by means of co-cultured inducing cells. However, the present cell co-culture systems limit the application of differentiated cells due to the safety and the scale of these systems. This study established a novel microcapsule co-culture system and investigated the probable effect of microencapsulated co-cultured neural cells on neural differentiation of mesenchymal stem cells (MSCs) ex vivo. Mouse bone marrow-derived MSCs were co-cultured with SHSY5Y cells microencapsulated in alginate-polylysine-alginate (APA) microcapsules. The quantitative and qualitative analysis showed that the expression of Nestin (Nes) was significantly increased at both gene and protein levels in the microcapsule co-culture system compared with other systems without cell co-culture, which indicated that more neural progenitors were obtained from this novel microcapsule co-culture system. According to our results and the unique characteristics of microcapsules, it is possible that large-scale cell co-culture system based on the combination of microcapsule and bioreactor technologies will be developed to generate numerous MSCs-derived neural progenitors for clinical applications in the future.
关键词[WOS]: TRANSPLANTATION ;  EXPRESSION ;  THERAPY ;  CULTURE ;  STROKE ;  GROWTH ;  INJURY ;  RAT
语种: 英语
WOS记录号: WOS:000318190200013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117649
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Dalian Med Univ, Hosp 2, Dept Orthoped, Dalian, Peoples R China
4.Dalian Med Univ, Collage Stomatol, Dept Oral Basic Sci, Dalian, Peoples R China
5.Dalian Med Univ, Lab Med Coll, Dalian, Peoples R China

Recommended Citation:
Liu, Yang,Liu, Chang,Nan, Feng,et al. Microcapsule Co-Culture System Enhances Neural Differentiation of Mesenchymal Stem Cells[J]. JOURNAL OF HARD TISSUE BIOLOGY,2013,22(2):241-247.
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