DICP OpenIR
Subject Area物理化学
Microcapsule Co-Culture System Enhances Neural Differentiation of Mesenchymal Stem Cells
Liu, Yang1; Liu, Chang1,2; Nan, Feng3; Xiao, Jing4; Lian, Jian-chun5; He, Xin5; Guo, Xin1; Sun, Guang-wei1; Ma, Xiao-jun1; Sun GW(孙广炜); Ma XJ(马小军)
KeywordCell Co-culture Mesenchymal Stem Cell Microcapsule Neural Differentiation
Source PublicationJOURNAL OF HARD TISSUE BIOLOGY
2013-04-01
Volume22Issue:2Pages:241-247
Indexed BySCI
SubtypeArticle
Department18
Funding Project1802
Contribution Rank1,1
WOS HeadingsScience & Technology ; Technology
WOS SubjectEngineering, Biomedical
WOS Research AreaEngineering
WOS KeywordTRANSPLANTATION ; EXPRESSION ; THERAPY ; CULTURE ; STROKE ; GROWTH ; INJURY ; RAT
AbstractCell co-culture plays a key role in inducing the committed differentiation of stem cells in vitro, which mimics the specific tissue microenvironment in vivo by means of co-cultured inducing cells. However, the present cell co-culture systems limit the application of differentiated cells due to the safety and the scale of these systems. This study established a novel microcapsule co-culture system and investigated the probable effect of microencapsulated co-cultured neural cells on neural differentiation of mesenchymal stem cells (MSCs) ex vivo. Mouse bone marrow-derived MSCs were co-cultured with SHSY5Y cells microencapsulated in alginate-polylysine-alginate (APA) microcapsules. The quantitative and qualitative analysis showed that the expression of Nestin (Nes) was significantly increased at both gene and protein levels in the microcapsule co-culture system compared with other systems without cell co-culture, which indicated that more neural progenitors were obtained from this novel microcapsule co-culture system. According to our results and the unique characteristics of microcapsules, it is possible that large-scale cell co-culture system based on the combination of microcapsule and bioreactor technologies will be developed to generate numerous MSCs-derived neural progenitors for clinical applications in the future.
Language英语
WOS IDWOS:000318190200013
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/117649
Collection中国科学院大连化学物理研究所
Corresponding AuthorSun GW(孙广炜); Ma XJ(马小军)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Dalian Med Univ, Hosp 2, Dept Orthoped, Dalian, Peoples R China
4.Dalian Med Univ, Collage Stomatol, Dept Oral Basic Sci, Dalian, Peoples R China
5.Dalian Med Univ, Lab Med Coll, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yang,Liu, Chang,Nan, Feng,et al. Microcapsule Co-Culture System Enhances Neural Differentiation of Mesenchymal Stem Cells[J]. JOURNAL OF HARD TISSUE BIOLOGY,2013,22(2):241-247.
APA Liu, Yang.,Liu, Chang.,Nan, Feng.,Xiao, Jing.,Lian, Jian-chun.,...&马小军.(2013).Microcapsule Co-Culture System Enhances Neural Differentiation of Mesenchymal Stem Cells.JOURNAL OF HARD TISSUE BIOLOGY,22(2),241-247.
MLA Liu, Yang,et al."Microcapsule Co-Culture System Enhances Neural Differentiation of Mesenchymal Stem Cells".JOURNAL OF HARD TISSUE BIOLOGY 22.2(2013):241-247.
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