DICP OpenIR
Subject Area物理化学
Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation
Ma, Ying1,2; Zhang, Ying1; Wang, Yu1,2; Wang, Qiuyan3; Tan, Mingqian1; Liu, Yang1; Chen, Li1,2; Li, Na1,2; Yu, Weiting1; Ma, Xiaojun1; Ma XJ(马小军)
KeywordMicroencapsulation Membrane Thickness Strength Permeability Cell Proliferation
Source PublicationJOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
2013-04-01
DOI10.1002/jbm.a.34395
Volume101Issue:4Pages:1007-1015
Indexed BySCI
SubtypeArticle
Department18
Funding Project1802
Contribution Rank1,1
WOS HeadingsScience & Technology ; Technology
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
WOS Research AreaEngineering ; Materials Science
WOS KeywordALGINATE POLYCATION MICROCAPSULES ; L-LYSINE MICROCAPSULES ; MICROENCAPSULATION ; OPTIMIZATION ; GROWTH ; TUMOR ; ENDOSTATIN ; STABILITY ; VIABILITY ; DELIVERY
AbstractCell microencapsulation is one of the promising strategies for in vitro production of proteins or in vivo delivery of therapeutic products. Membrane thickness controls microcapsule strength and permeability, which may in return affect cell growth and metabolism. In this study, the strength, permeability, and encapsulated Chinese hamster ovary cell proliferation and metabolism of four groups of microcapsules with different membrane thicknesses were investigated. It was found that increasing membrane thickness increases microcapsule strength, whereas decreases membrane permeability. During the first 6 days, cells within microcapsules with 10 m thickness membrane proliferated fast and could reach a cell density of 1.9 x 107 cells/mL microcapsule with 92% cell density. A cell density of 5.5 x 107 cells/mL microcapsule with >85% cell density was achieved within microcapsules with 15 m membrane thickness and these microcapsules kept over 88% integrity ratio after 11 days, which was much higher than that of microcapsules with 10 m membrane thickness. Membrane with more than 20 m thickness was not suited for encapsulated cell culture owing to low-protein diffusion rate. These results indicated that cells survived shortly within the thinnest membrane thickness. There was a specific membrane thickness more suitable for cell growth for a long-time culture. These findings will be useful for preparing microcapsules with the desired membrane thickness for microencapsulated cell culture dependent on various purposes. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
Language英语
WOS IDWOS:000315406300011
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/117670
Collection中国科学院大连化学物理研究所
Corresponding AuthorMa XJ(马小军)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dept Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Liaoning Entry Exit Inspect & Quarantine Bur, Dalian 116001, Peoples R China
Recommended Citation
GB/T 7714
Ma, Ying,Zhang, Ying,Wang, Yu,et al. Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2013,101(4):1007-1015.
APA Ma, Ying.,Zhang, Ying.,Wang, Yu.,Wang, Qiuyan.,Tan, Mingqian.,...&马小军.(2013).Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,101(4),1007-1015.
MLA Ma, Ying,et al."Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 101.4(2013):1007-1015.
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