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学科主题: 物理化学
题名: Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation
作者: Ma, Ying1, 2;  Zhang, Ying1;  Wang, Yu1, 2;  Wang, Qiuyan3;  Tan, Mingqian1;  Liu, Yang1;  Chen, Li1, 2;  Li, Na1, 2;  Yu, Weiting1;  Ma, Xiaojun1
通讯作者: 马小军
关键词: microencapsulation ;  membrane thickness ;  strength ;  permeability ;  cell proliferation
刊名: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
发表日期: 2013-04-01
DOI: 10.1002/jbm.a.34395
卷: 101, 期:4, 页:1007-1015
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 1,1
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Engineering, Biomedical ;  Materials Science, Biomaterials
研究领域[WOS]: Engineering ;  Materials Science
英文摘要: Cell microencapsulation is one of the promising strategies for in vitro production of proteins or in vivo delivery of therapeutic products. Membrane thickness controls microcapsule strength and permeability, which may in return affect cell growth and metabolism. In this study, the strength, permeability, and encapsulated Chinese hamster ovary cell proliferation and metabolism of four groups of microcapsules with different membrane thicknesses were investigated. It was found that increasing membrane thickness increases microcapsule strength, whereas decreases membrane permeability. During the first 6 days, cells within microcapsules with 10 m thickness membrane proliferated fast and could reach a cell density of 1.9 x 107 cells/mL microcapsule with 92% cell density. A cell density of 5.5 x 107 cells/mL microcapsule with >85% cell density was achieved within microcapsules with 15 m membrane thickness and these microcapsules kept over 88% integrity ratio after 11 days, which was much higher than that of microcapsules with 10 m membrane thickness. Membrane with more than 20 m thickness was not suited for encapsulated cell culture owing to low-protein diffusion rate. These results indicated that cells survived shortly within the thinnest membrane thickness. There was a specific membrane thickness more suitable for cell growth for a long-time culture. These findings will be useful for preparing microcapsules with the desired membrane thickness for microencapsulated cell culture dependent on various purposes. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
关键词[WOS]: ALGINATE POLYCATION MICROCAPSULES ;  L-LYSINE MICROCAPSULES ;  MICROENCAPSULATION ;  OPTIMIZATION ;  GROWTH ;  TUMOR ;  ENDOSTATIN ;  STABILITY ;  VIABILITY ;  DELIVERY
语种: 英语
WOS记录号: WOS:000315406300011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117670
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dept Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Liaoning Entry Exit Inspect & Quarantine Bur, Dalian 116001, Peoples R China

Recommended Citation:
Ma, Ying,Zhang, Ying,Wang, Yu,et al. Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2013,101(4):1007-1015.
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