DICP OpenIR
学科主题物理化学
Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation
Ma, Ying1,2; Zhang, Ying1; Wang, Yu1,2; Wang, Qiuyan3; Tan, Mingqian1; Liu, Yang1; Chen, Li1,2; Li, Na1,2; Yu, Weiting1; Ma, Xiaojun1; Ma XJ(马小军)
关键词Microencapsulation Membrane Thickness Strength Permeability Cell Proliferation
刊名JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
2013-04-01
DOI10.1002/jbm.a.34395
101期:4页:1007-1015
收录类别SCI
文章类型Article
部门归属18
项目归属1802
产权排名1,1
WOS标题词Science & Technology ; Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]ALGINATE POLYCATION MICROCAPSULES ; L-LYSINE MICROCAPSULES ; MICROENCAPSULATION ; OPTIMIZATION ; GROWTH ; TUMOR ; ENDOSTATIN ; STABILITY ; VIABILITY ; DELIVERY
英文摘要Cell microencapsulation is one of the promising strategies for in vitro production of proteins or in vivo delivery of therapeutic products. Membrane thickness controls microcapsule strength and permeability, which may in return affect cell growth and metabolism. In this study, the strength, permeability, and encapsulated Chinese hamster ovary cell proliferation and metabolism of four groups of microcapsules with different membrane thicknesses were investigated. It was found that increasing membrane thickness increases microcapsule strength, whereas decreases membrane permeability. During the first 6 days, cells within microcapsules with 10 m thickness membrane proliferated fast and could reach a cell density of 1.9 x 107 cells/mL microcapsule with 92% cell density. A cell density of 5.5 x 107 cells/mL microcapsule with >85% cell density was achieved within microcapsules with 15 m membrane thickness and these microcapsules kept over 88% integrity ratio after 11 days, which was much higher than that of microcapsules with 10 m membrane thickness. Membrane with more than 20 m thickness was not suited for encapsulated cell culture owing to low-protein diffusion rate. These results indicated that cells survived shortly within the thinnest membrane thickness. There was a specific membrane thickness more suitable for cell growth for a long-time culture. These findings will be useful for preparing microcapsules with the desired membrane thickness for microencapsulated cell culture dependent on various purposes. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
语种英语
WOS记录号WOS:000315406300011
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/117670
专题中国科学院大连化学物理研究所
通讯作者Ma XJ(马小军)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dept Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Liaoning Entry Exit Inspect & Quarantine Bur, Dalian 116001, Peoples R China
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GB/T 7714
Ma, Ying,Zhang, Ying,Wang, Yu,et al. Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2013,101(4):1007-1015.
APA Ma, Ying.,Zhang, Ying.,Wang, Yu.,Wang, Qiuyan.,Tan, Mingqian.,...&马小军.(2013).Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,101(4),1007-1015.
MLA Ma, Ying,et al."Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 101.4(2013):1007-1015.
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