DICP OpenIR
Subject Area物理化学
Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix
Zhao, Yanyan1,2; Li, Xiuling1; Yan, Jingyu1; Guo, Zhimou1; Liang, Xinmiao1; Li XL(李秀玲); Liang XM(梁鑫淼)
Source PublicationANALYTICAL METHODS
2012-05-01
ISSN1759-9660
DOI10.1039/c2ay05915k
Volume4Issue:5Pages:1244-1251
Indexed BySCI
SubtypeArticle
Department18
Funding Project1803
Contribution Rank1,1
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine ; Technology
WOS SubjectChemistry, Analytical ; Food Science & Technology ; Spectroscopy
WOS Research AreaChemistry ; Food Science & Technology ; Spectroscopy
WOS KeywordINTERACTION LIQUID-CHROMATOGRAPHY ; METAL AFFINITY-CHROMATOGRAPHY ; HIGHLY SELECTIVE ENRICHMENT ; PHOSPHOPROTEOME ANALYSIS ; PHOSPHORYLATED PEPTIDES ; MASS-SPECTROMETRY ; TITANIUM-DIOXIDE ; EXCHANGE ; ANION ; IDENTIFICATION
AbstractReversible protein phosphorylation regulates many significant cellular processes. Identification of phosphorylation sites is vital to elucidate their biofunctions. To aid in phosphoproteome characterization, several selective enrichment methods have been developed, including immobilized metal-ion affinity chromatography (IMAC) and titanium dioxide (TiO2). However, the high pH elution step applied in these two methods, which tends to degrade phosphopeptides. In order to improve phosphopeptide enrichment efficiency, a hydrophilic interaction liquid chromatography (HILIC) based material, cyclodextrin (CD) bonded silica (Click OEG-CD), was synthesized in our group and applied to phosphopeptide enrichment and fractionation. Taking tryptic digest of standard protein a-casein as model sample, the performance of Click OEG-CD in phosphopeptide isolation was investigated. It was found that both the acetonitrile and salt concentrations in mobile phase influence the phosphopeptide enrichment selectivity of the matrix. Under optimized enrichment condition, Click OEG-CD has a similar phosphopeptide enrichment selectivity as commercial TiO2. Meanwhile, phosphopeptides with same charges could be fractionated according to hydrophilicity difference on Click OEG-CD under acetonitrile gradient. Different selectivity is proved with Click OEG-CD from conventional SAX. We demonstrate that Click OEG-CD is an efficient matrix in phosphopeptide enrichment and fractionation.
Language英语
WOS IDWOS:000303603000009
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/117720
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi XL(李秀玲); Liang XM(梁鑫淼)
Affiliation1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Yanyan,Li, Xiuling,Yan, Jingyu,et al. Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix[J]. ANALYTICAL METHODS,2012,4(5):1244-1251.
APA Zhao, Yanyan.,Li, Xiuling.,Yan, Jingyu.,Guo, Zhimou.,Liang, Xinmiao.,...&梁鑫淼.(2012).Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix.ANALYTICAL METHODS,4(5),1244-1251.
MLA Zhao, Yanyan,et al."Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix".ANALYTICAL METHODS 4.5(2012):1244-1251.
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