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学科主题: 物理化学
题名: Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix
作者: Zhao, Yanyan1, 2;  Li, Xiuling1;  Yan, Jingyu1;  Guo, Zhimou1;  Liang, Xinmiao1
通讯作者: 李秀玲 ;  梁鑫淼
刊名: ANALYTICAL METHODS
发表日期: 2012-05-01
DOI: 10.1039/c2ay05915k
卷: 4, 期:5, 页:1244-1251
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Chemistry, Analytical ;  Food Science & Technology ;  Spectroscopy
研究领域[WOS]: Chemistry ;  Food Science & Technology ;  Spectroscopy
英文摘要: Reversible protein phosphorylation regulates many significant cellular processes. Identification of phosphorylation sites is vital to elucidate their biofunctions. To aid in phosphoproteome characterization, several selective enrichment methods have been developed, including immobilized metal-ion affinity chromatography (IMAC) and titanium dioxide (TiO2). However, the high pH elution step applied in these two methods, which tends to degrade phosphopeptides. In order to improve phosphopeptide enrichment efficiency, a hydrophilic interaction liquid chromatography (HILIC) based material, cyclodextrin (CD) bonded silica (Click OEG-CD), was synthesized in our group and applied to phosphopeptide enrichment and fractionation. Taking tryptic digest of standard protein a-casein as model sample, the performance of Click OEG-CD in phosphopeptide isolation was investigated. It was found that both the acetonitrile and salt concentrations in mobile phase influence the phosphopeptide enrichment selectivity of the matrix. Under optimized enrichment condition, Click OEG-CD has a similar phosphopeptide enrichment selectivity as commercial TiO2. Meanwhile, phosphopeptides with same charges could be fractionated according to hydrophilicity difference on Click OEG-CD under acetonitrile gradient. Different selectivity is proved with Click OEG-CD from conventional SAX. We demonstrate that Click OEG-CD is an efficient matrix in phosphopeptide enrichment and fractionation.
关键词[WOS]: INTERACTION LIQUID-CHROMATOGRAPHY ;  METAL AFFINITY-CHROMATOGRAPHY ;  HIGHLY SELECTIVE ENRICHMENT ;  PHOSPHOPROTEOME ANALYSIS ;  PHOSPHORYLATED PEPTIDES ;  MASS-SPECTROMETRY ;  TITANIUM-DIOXIDE ;  EXCHANGE ;  ANION ;  IDENTIFICATION
语种: 英语
WOS记录号: WOS:000303603000009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117720
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China

Recommended Citation:
Zhao, Yanyan,Li, Xiuling,Yan, Jingyu,et al. Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix[J]. ANALYTICAL METHODS,2012,4(5):1244-1251.
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