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学科主题物理化学
Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix
Zhao, Yanyan1,2; Li, Xiuling1; Yan, Jingyu1; Guo, Zhimou1; Liang, Xinmiao1; Li XL(李秀玲); Liang XM(梁鑫淼)
刊名ANALYTICAL METHODS
2012-05-01
ISSN1759-9660
DOI10.1039/c2ay05915k
4期:5页:1244-1251
收录类别SCI
文章类型Article
部门归属18
项目归属1803
产权排名1,1
WOS标题词Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine ; Technology
类目[WOS]Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
研究领域[WOS]Chemistry ; Food Science & Technology ; Spectroscopy
关键词[WOS]INTERACTION LIQUID-CHROMATOGRAPHY ; METAL AFFINITY-CHROMATOGRAPHY ; HIGHLY SELECTIVE ENRICHMENT ; PHOSPHOPROTEOME ANALYSIS ; PHOSPHORYLATED PEPTIDES ; MASS-SPECTROMETRY ; TITANIUM-DIOXIDE ; EXCHANGE ; ANION ; IDENTIFICATION
英文摘要Reversible protein phosphorylation regulates many significant cellular processes. Identification of phosphorylation sites is vital to elucidate their biofunctions. To aid in phosphoproteome characterization, several selective enrichment methods have been developed, including immobilized metal-ion affinity chromatography (IMAC) and titanium dioxide (TiO2). However, the high pH elution step applied in these two methods, which tends to degrade phosphopeptides. In order to improve phosphopeptide enrichment efficiency, a hydrophilic interaction liquid chromatography (HILIC) based material, cyclodextrin (CD) bonded silica (Click OEG-CD), was synthesized in our group and applied to phosphopeptide enrichment and fractionation. Taking tryptic digest of standard protein a-casein as model sample, the performance of Click OEG-CD in phosphopeptide isolation was investigated. It was found that both the acetonitrile and salt concentrations in mobile phase influence the phosphopeptide enrichment selectivity of the matrix. Under optimized enrichment condition, Click OEG-CD has a similar phosphopeptide enrichment selectivity as commercial TiO2. Meanwhile, phosphopeptides with same charges could be fractionated according to hydrophilicity difference on Click OEG-CD under acetonitrile gradient. Different selectivity is proved with Click OEG-CD from conventional SAX. We demonstrate that Click OEG-CD is an efficient matrix in phosphopeptide enrichment and fractionation.
语种英语
WOS记录号WOS:000303603000009
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/117720
专题中国科学院大连化学物理研究所
通讯作者Li XL(李秀玲); Liang XM(梁鑫淼)
作者单位1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Yanyan,Li, Xiuling,Yan, Jingyu,et al. Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix[J]. ANALYTICAL METHODS,2012,4(5):1244-1251.
APA Zhao, Yanyan.,Li, Xiuling.,Yan, Jingyu.,Guo, Zhimou.,Liang, Xinmiao.,...&梁鑫淼.(2012).Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix.ANALYTICAL METHODS,4(5),1244-1251.
MLA Zhao, Yanyan,et al."Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix".ANALYTICAL METHODS 4.5(2012):1244-1251.
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