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DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry
作者: Zhang, Xihao1, 2;  He, Xiwen1, 2;  Chen, Langxing1, 2;  Zhang, Yukui1, 2, 3
通讯作者: 陈朗星
刊名: JOURNAL OF MATERIALS CHEMISTRY
发表日期: 2012
DOI: 10.1039/c2jm32987e
卷: 22, 期:32, 页:16520-16526
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 3,4
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Physical ;  Materials Science, Multidisciplinary
研究领域[WOS]: Chemistry ;  Materials Science
英文摘要: In this study, a novel approach was developed to synthesize aminophenylboronic acid functionalized magnetic nanoparticles (NPs) via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) 'click' chemistry. Firstly, azide-functional Fe3O4 NPs were obtained by a two-step chemical modification process. Then, an alkyne-phenylboronic acid molecule was connected onto the surface of magnetite by the CuAAC reaction. The morphology, structure and composition of the synthesized nanocomposites were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), vibrating sample magnetometer (VSM), Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectrometry (XPS). Five proteins, including ovalbumin (OB), transferrin (Trf), as glycoprotein templates and lysozyme (Lyz), bovine serum albumin (BSA), horse heart cytochrome c (Cyt C) as nonglycoprotein templates are chosen as target proteins. The as-prepared click-Fe3O4@APBA NPs with a mean diameter of 23.2 nm showed a strong magnetic response to an externally applied magnetic field and exhibited a high adsorption capacity and excellent specificity towards glycoproteins in comparison with nonglycoproteins. The click-Fe3O4@APBA NPs showed the higher adsorption capacity towards glycoproteins than the nonclick-Fe3O4@APBA NPs which were synthesized through a common nucleophilic substitution reaction. The greatly enhanced adsorption capacity towards glycoproteins demonstrated that the 'click' method presented great superiority in ligand immobilization. Finally, the click-Fe3O4@APBA NPs could efficiently enrich glycoproteins from real egg white samples as well.
关键词[WOS]: AFFINITY MONOLITHIC COLUMN ;  IRON-OXIDE NANOPARTICLES ;  ONE-POT SYNTHESIS ;  SELECTIVE ENRICHMENT ;  GOLD NANOPARTICLES ;  PROTEIN SEPARATION ;  MESOPOROUS SILICA ;  FACILE SYNTHESIS ;  GLYCOPEPTIDES ;  GLYCOSYLATION
语种: 英语
WOS记录号: WOS:000306708700051
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117833
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Zhang, Xihao,He, Xiwen,Chen, Langxing,et al. Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry[J]. JOURNAL OF MATERIALS CHEMISTRY,2012,22(32):16520-16526.
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