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学科主题物理化学
Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry
Zhang, Xihao1,2; He, Xiwen1,2; Chen, Langxing1,2; Zhang, Yukui1,2,3; Chen LX(陈朗星)
刊名JOURNAL OF MATERIALS CHEMISTRY
2012
DOI10.1039/c2jm32987e
22期:32页:16520-16526
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名3,4
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Physical ; Materials Science, Multidisciplinary
研究领域[WOS]Chemistry ; Materials Science
关键词[WOS]AFFINITY MONOLITHIC COLUMN ; IRON-OXIDE NANOPARTICLES ; ONE-POT SYNTHESIS ; SELECTIVE ENRICHMENT ; GOLD NANOPARTICLES ; PROTEIN SEPARATION ; MESOPOROUS SILICA ; FACILE SYNTHESIS ; GLYCOPEPTIDES ; GLYCOSYLATION
英文摘要In this study, a novel approach was developed to synthesize aminophenylboronic acid functionalized magnetic nanoparticles (NPs) via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) 'click' chemistry. Firstly, azide-functional Fe3O4 NPs were obtained by a two-step chemical modification process. Then, an alkyne-phenylboronic acid molecule was connected onto the surface of magnetite by the CuAAC reaction. The morphology, structure and composition of the synthesized nanocomposites were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), vibrating sample magnetometer (VSM), Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectrometry (XPS). Five proteins, including ovalbumin (OB), transferrin (Trf), as glycoprotein templates and lysozyme (Lyz), bovine serum albumin (BSA), horse heart cytochrome c (Cyt C) as nonglycoprotein templates are chosen as target proteins. The as-prepared click-Fe3O4@APBA NPs with a mean diameter of 23.2 nm showed a strong magnetic response to an externally applied magnetic field and exhibited a high adsorption capacity and excellent specificity towards glycoproteins in comparison with nonglycoproteins. The click-Fe3O4@APBA NPs showed the higher adsorption capacity towards glycoproteins than the nonclick-Fe3O4@APBA NPs which were synthesized through a common nucleophilic substitution reaction. The greatly enhanced adsorption capacity towards glycoproteins demonstrated that the 'click' method presented great superiority in ligand immobilization. Finally, the click-Fe3O4@APBA NPs could efficiently enrich glycoproteins from real egg white samples as well.
语种英语
WOS记录号WOS:000306708700051
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/117833
专题中国科学院大连化学物理研究所
通讯作者Chen LX(陈朗星)
作者单位1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Xihao,He, Xiwen,Chen, Langxing,et al. Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry[J]. JOURNAL OF MATERIALS CHEMISTRY,2012,22(32):16520-16526.
APA Zhang, Xihao,He, Xiwen,Chen, Langxing,Zhang, Yukui,&陈朗星.(2012).Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry.JOURNAL OF MATERIALS CHEMISTRY,22(32),16520-16526.
MLA Zhang, Xihao,et al."Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry".JOURNAL OF MATERIALS CHEMISTRY 22.32(2012):16520-16526.
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