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学科主题: 物理化学
题名: Nano-flow multidimensional liquid chromatography platform integrated with combination of protein and peptide separation for proteome analysis
作者: Xia, Simin1, 2;  Tao, Dingyin1, 2;  Yuan, Huiming1;  Zhou, Yuan1, 2;  Liang, Zhen1;  Zhang, Lihua1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Immobilized enzymatic reactor ;  Multidimensional chromatography ;  Nano-flow LC ;  Online digestion ;  Proteome
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2012-07-01
DOI: 10.1002/jssc.201200052
卷: 35, 期:14, 页:1764-1770
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: An integrated multidimensional nano-flow liquid chromatography platform with the combination of protein and peptide separation via online digestion by an immobilized enzymatic reactor was established, and successfully applied for proteome analysis. By this platform, proteins were first separated by a weak anion and weak cation mixed-bed microcolumn under a series of salt steps, online digested by a trypsin immobilized enzymatic reactor, digests trapped and desalted by a C18 precolumn, separated by nano-reversed phase liquid chromatography, and finally identified by electrospray ionization-MS/MS. To evaluate the performance of such a platform, Escherichia coli whole cell lysate proteins were analyzed. Compared with the results obtained by shotgun approach, the identified protein number was increased by 6%, with the consumed time decreased from 38 to 14 h. We also compared with integrate platform based on micro-HPLC, and the required sample amount was decreased to 8 mu g. These results demonstrated that such an integrated approach would be an attractive alternative to commonly applied approaches for proteome research.
关键词[WOS]: TOP-DOWN PROTEOMICS ;  MONOLITH ;  COLUMN
语种: 英语
WOS记录号: WOS:000306493700009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117846
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Xia, Simin,Tao, Dingyin,Yuan, Huiming,et al. Nano-flow multidimensional liquid chromatography platform integrated with combination of protein and peptide separation for proteome analysis[J]. JOURNAL OF SEPARATION SCIENCE,2012,35(14):1764-1770.
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