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学科主题物理化学
Nano-flow multidimensional liquid chromatography platform integrated with combination of protein and peptide separation for proteome analysis
Xia, Simin1,2; Tao, Dingyin1,2; Yuan, Huiming1; Zhou, Yuan1,2; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
关键词Immobilized Enzymatic Reactor Multidimensional Chromatography Nano-flow Lc Online Digestion Proteome
刊名JOURNAL OF SEPARATION SCIENCE
2012-07-01
DOI10.1002/jssc.201200052
35期:14页:1764-1770
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1,1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]TOP-DOWN PROTEOMICS ; MONOLITH ; COLUMN
英文摘要An integrated multidimensional nano-flow liquid chromatography platform with the combination of protein and peptide separation via online digestion by an immobilized enzymatic reactor was established, and successfully applied for proteome analysis. By this platform, proteins were first separated by a weak anion and weak cation mixed-bed microcolumn under a series of salt steps, online digested by a trypsin immobilized enzymatic reactor, digests trapped and desalted by a C18 precolumn, separated by nano-reversed phase liquid chromatography, and finally identified by electrospray ionization-MS/MS. To evaluate the performance of such a platform, Escherichia coli whole cell lysate proteins were analyzed. Compared with the results obtained by shotgun approach, the identified protein number was increased by 6%, with the consumed time decreased from 38 to 14 h. We also compared with integrate platform based on micro-HPLC, and the required sample amount was decreased to 8 mu g. These results demonstrated that such an integrated approach would be an attractive alternative to commonly applied approaches for proteome research.
语种英语
WOS记录号WOS:000306493700009
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/117846
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
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GB/T 7714
Xia, Simin,Tao, Dingyin,Yuan, Huiming,et al. Nano-flow multidimensional liquid chromatography platform integrated with combination of protein and peptide separation for proteome analysis[J]. JOURNAL OF SEPARATION SCIENCE,2012,35(14):1764-1770.
APA Xia, Simin.,Tao, Dingyin.,Yuan, Huiming.,Zhou, Yuan.,Liang, Zhen.,...&张丽华.(2012).Nano-flow multidimensional liquid chromatography platform integrated with combination of protein and peptide separation for proteome analysis.JOURNAL OF SEPARATION SCIENCE,35(14),1764-1770.
MLA Xia, Simin,et al."Nano-flow multidimensional liquid chromatography platform integrated with combination of protein and peptide separation for proteome analysis".JOURNAL OF SEPARATION SCIENCE 35.14(2012):1764-1770.
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