中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Identification, Characterization, and Epitope Mapping of Human Monoclonal Antibody J19 That Specifically Recognizes Activated Integrin alpha(4)beta(7)
作者: Qi, JunPeng1;  Zhang, Kun1;  Zhang, Qiao1;  Sun, Yi1;  Fu, Ting2;  Li, GuoHui2;  Chen, JianFeng1
通讯作者: ChenJianFeng
刊名: JOURNAL OF BIOLOGICAL CHEMISTRY
发表日期: 2012-05-04
DOI: 10.1074/jbc.M112.341263
卷: 287, 期:19, 页:15749-15759
收录类别: SCI
文章类型: Article
部门归属: 11
项目归属: 1106
产权排名: 2,5
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Integrin alpha(4)beta(7) is a lymphocyte homing receptor that mediates both rolling and firm adhesion of lymphocytes on vascular endothelium, two of the critical steps in lymphocyte migration and tissue-specific homing. The rolling and firm adhesions of lymphocytes rely on the dynamic shift between the inactive and active states of integrin alpha(4)beta(7), which is associated with the conformational rearrangement of integrin molecules. Activation-specific antibodies, which specifically recognize the activated integrins, have been used as powerful tools in integrin studies, whereas there is no well characterized activation-specific antibody to integrin alpha(4)beta(7). Here, we report the identification, characterization, and epitope mapping of an activation-specific human mAb J19 against integrin alpha(4)beta(7). J19 was discovered by screening a human single-chain variable fragment phage library using an activated alpha(4)beta(7) mutant as target. J19 IgG specifically bound to the high affinity alpha(4)beta(7) induced by Mn2+, DTT, ADP, or CXCL12, but not to the low affinity integrin. Moreover, J19 IgG did not interfere with alpha(4)beta(7)-MAdCAM-1 interaction. The epitope of J19 IgG was mapped to Ser-331, Ala-332, and Ala-333 of beta(7) I domain and a seven-residue segment from 184 to 190 of alpha(4)beta-propeller domain, which are buried in low affinity integrin with bent conformation and only exposed in the high affinity extended conformation. Taken together, J19 is a potentially powerful tool for both studies on alpha(4)beta(7) activation mechanism and development of novel therapeutics targeting the activated lymphocyte expressing high affinity alpha(4)beta(7).
关键词[WOS]: POLYMERASE CHAIN-REACTION ;  ALPHA-I DOMAIN ;  LYMPHOCYTE MIGRATION ;  AVIDITY REGULATION ;  OVERLAP EXTENSION ;  STRUCTURAL BASIS ;  PHORBOL ESTER ;  CELL-ADHESION ;  FIRM ADHESION ;  OUTSIDE-IN
语种: 英语
WOS记录号: WOS:000304006300053
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117857
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2012TgNyReX9uA.PDF(3065KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China
2.Chinese Acad Sci, Lab Mol Modeling & Design, State Key Lab Mol React Dynam, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Qi, JunPeng,Zhang, Kun,Zhang, Qiao,et al. Identification, Characterization, and Epitope Mapping of Human Monoclonal Antibody J19 That Specifically Recognizes Activated Integrin alpha(4)beta(7)[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2012,287(19):15749-15759.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Qi, JunPeng]'s Articles
 [Zhang, Kun]'s Articles
 [Zhang, Qiao]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Qi, JunPeng]‘s Articles
 [Zhang, Kun]‘s Articles
 [Zhang, Qiao]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2012TgNyReX9uA.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace