中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Hormone-responsive 3D multicellular culture model of human breast tissue
作者: Wang, Xiuli1, 2;  Kaplan, David L.1
通讯作者: DavidLKaplan
关键词: 3D tissue ;  Silk ;  Breast ;  Casein ;  Epithelial cells ;  Morphogenesis
刊名: BIOMATERIALS
发表日期: 2012-04-01
DOI: 10.1016/j.biomaterials.2012.01.011
卷: 33, 期:12, 页:3411-3420
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1806
产权排名: 2, 1
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Engineering, Biomedical ;  Materials Science, Biomaterials
研究领域[WOS]: Engineering ;  Materials Science
英文摘要: A hormone-responsive 3D human tissue-like culture system was developed in which human primary mammary epithelial cells (MECs) were co-cultured with two types of predominant mammary stromal cells on silk protein scaffolds. Silk porous scaffolds with incorporated extracellular matrix provided a compatible environment for epithelial structure morphogenesis and differentiation. The presence of stromal cells promoted MEC proliferation, induced both alveolar and ductal morphogenesis and enhanced casein expression. In contrast, only alveolar structures were observed in monocultures. The alveolar structures generated from the heterotypic cultures in vitro exhibited proper polarity similar to human breast tissue in vivo. Consistent with their phenotypic appearance, more functional differentiation of epithelial cells was also observed in the heterotypic cultures, where casein-alpha and -beta mRNA expression were increased significantly. Additionally, this 3D multicellular culture model displayed an estrogen-responsive physiologically relevant response, evidenced by enhanced cell proliferation, aberrant morphology, changes in gene expression profile and few polarized lumen structures after estrogen treatment. This culture system offers an excellent opportunity to explore the role of cell-cell and cell-substrate interactions during mammary gland development, the consequences of hormone receptor activation on MEC behavior and morphogenesis, as well as their alteration during neoplastic transformation in human breast tissue. (C) 2012 Elsevier Ltd. All rights reserved.
关键词[WOS]: MAMMARY EPITHELIAL-CELLS ;  HEPATOCYTE GROWTH-FACTOR ;  BASEMENT-MEMBRANE CULTURES ;  MESENCHYMAL STEM-CELLS ;  IN-VITRO ;  ALVEOLAR MORPHOGENESIS ;  TUMOR PROGRESSION ;  ESTROGEN ;  PROLIFERATION ;  FIBROBLASTS
语种: 英语
WOS记录号: WOS:000301886300004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117862
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2012tdJXi3EuMT.PDF(1252KB)----开放获取--View Download

作者单位: 1.Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Xiuli,Kaplan, David L.. Hormone-responsive 3D multicellular culture model of human breast tissue[J]. BIOMATERIALS,2012,33(12):3411-3420.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Wang, Xiuli]'s Articles
 [Kaplan, David L.]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Wang, Xiuli]‘s Articles
 [Kaplan, David L.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2012tdJXi3EuMT.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace