中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Microalgal motility measurement microfluidic chip for toxicity assessment of heavy metals
作者: Zheng, Guoxia1, 2;  Wang, Yunhua2;  Qin, Jianhua1
通讯作者: 秦建华
关键词: Microfluidic chip ;  Marine microalgae ;  Motility ;  Toxicity assessment ;  Chemostatic module
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2012-12-01
DOI: 10.1007/s00216-012-6408-6
卷: 404, 期:10, 页:3061-3069
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A polydimethylsiloxane microfluidic chip has been developed for the estimation of toxic heavy metals based on measurement of mobility of marine microalgae. The chip is mainly composed of an upstream concentration gradient generator and a downstream perfusion-based chemotatic module. The processes of toxic liquid dilution and diffusion, microalgal culturing, cell stimulation, and online screening can be integrated in this chip, which makes it an attractive approach to simplify toxicity testing procedures. The microalgal motility was adopted as a microfluidic bioassay signal and was evaluated as the percentage of motile cells, curvilinear velocity, average path velocity, and straight line velocity. Two mobile marine microalgae, Platymonas subcordiformis and Platymonas helgolandica var. tsingtaoensis, were confined in the chemotatic module and stimulated by the eight concentration gradients of Cu and Cd generated by the concentration gradient generator. In all cases, a toxic response was detected (i.e., a dose-related inhibition of motility was observed). Only 1.5 h was needed to predict EC50 values. Thus, the microfluidic chip developed was proved to be useful as a simple and rapid approach in heavy metal detection and might be expanded as a conventional test method in environmental toxicity assessment.
关键词[WOS]: MARINE MICROALGAE ;  SPERM MOTILITY ;  ALGAE ;  COPPER ;  CADMIUM ;  SYSTEMS ;  POLLUTANTS ;  BIOSENSOR ;  BACTERIAL ;  GROWTH
语种: 英语
WOS记录号: WOS:000311310100028
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117872
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2012t4dnWXfe1D.PDF(531KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Dept Biotechnol, Dalian Inst Chem Phys, Dalian 116622, Liaoning, Peoples R China
2.Dalian Univ, Dalian 116622, Liaoning, Peoples R China

Recommended Citation:
Zheng, Guoxia,Wang, Yunhua,Qin, Jianhua. Microalgal motility measurement microfluidic chip for toxicity assessment of heavy metals[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2012,404(10):3061-3069.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Zheng, Guoxia]'s Articles
 [Wang, Yunhua]'s Articles
 [Qin, Jianhua]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Zheng, Guoxia]‘s Articles
 [Wang, Yunhua]‘s Articles
 [Qin, Jianhua]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2012t4dnWXfe1D.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace