DICP OpenIR
Subject Area物理化学
Preparation and chromatographic evaluation of a cysteine-bonded zwitterionic hydrophilic interaction liquid chromatography stationary phase
Shen, Aijin; Guo, Zhimou; Cai, Xiaoming; Xue, Xingya; Liang, Xinmiao; Liang XM(梁鑫淼); Guo ZM(郭志谋)
KeywordHydrophilic Interaction Chromatography "thiol-ene" Click Chemistry Zwitterionic Stationary Phase Cysteine 2d-lc Orthogonality
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2012-03-09
DOI10.1016/j.chroma.2011.10.086
Volume1228Pages:175-182
Indexed BySCI
SubtypeArticle
Department18
Funding Project1803
Contribution Rank1,1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordMIXED TRIFUNCTIONAL SILANES ; CLICK-CHEMISTRY ; POLAR COMPOUNDS ; MASS-SPECTROMETRY ; SILICA-GEL ; HORIZONTAL POLYMERIZATION ; REVERSED-PHASE ; HPLC SYSTEM ; SEPARATION ; RETENTION
AbstractA cysteine-bonded zwitterionic hydrophilic interaction chromatography (HILIC) stationary phase (Click TE-Cys) was prepared based on the "thiol-ene" click chemistry. The Click TE-Cys material was characterized by solid state C-13 cross polarization/magic-angle spinning (CP/MAS) NMR and elemental analysis. The dynamic evaluation for cytosine, cytidine and orotic acid was performed using Van Deemter plots. The plate height values were no more than 24 mu m for the flow rate between 0.5 and 5.4 mm s(-1) (0.3-3.5 mL min(-1)), which proved the excellent separation efficiency of Click TE-Cys stationary phase. The influences of the content of water, concentration of salt and pH of the buffer solution on the retention of model compounds were investigated. The results demonstrated that the separation of polar analytes was dominated by the partitioning mechanism, while the contribution of electrostatic interaction was minor. The thermodynamic characteristic of Click TE-Cys stationary phase was also studied according to van't Hoff plot. An exothermic process for transferring analytes from the mobile phase to the stationary phase was observed and a linear relationship for Ink and 1/T was achieved, indicating no change of retention mechanism within the measured temperature range. Besides, the zwitterionic stationary phase exhibited good stability. Considering the high hydrophilicity of Click TE-Cys stationary phase, the application in the separation of protein tryptic digests was carried out using hydrophilic interaction chromatography-electrospray ionization mass spectrometry (HILIC-ESI-MS). More peaks were adequately resolved on the Click TE-Cys column comparing with that on the TSK Amide-80 column. In addition, the orthogonality between HILIC and RPLC system was investigated utilizing geometric approach. The XTerra MS C-18 and Click TE-Cys column displayed great difference in separation selectivity, with the orthogonality reaching 88.0%. On the other hand, the orthogonality between Click TE-Cys and TSK Amide-80 system was 21.4%, i.e. the selectivity was similar but slightly different from each other. The successful separation of protein digests indicated the great potential of Click TE-Cys stationary phase in the separation of complex samples and applicability in two-dimensional liquid chromatography (2D-LC). (C) 2011 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000301406100017
Citation statistics
Cited Times:80[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/117880
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiang XM(梁鑫淼); Guo ZM(郭志谋)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Shen, Aijin,Guo, Zhimou,Cai, Xiaoming,et al. Preparation and chromatographic evaluation of a cysteine-bonded zwitterionic hydrophilic interaction liquid chromatography stationary phase[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1228:175-182.
APA Shen, Aijin.,Guo, Zhimou.,Cai, Xiaoming.,Xue, Xingya.,Liang, Xinmiao.,...&郭志谋.(2012).Preparation and chromatographic evaluation of a cysteine-bonded zwitterionic hydrophilic interaction liquid chromatography stationary phase.JOURNAL OF CHROMATOGRAPHY A,1228,175-182.
MLA Shen, Aijin,et al."Preparation and chromatographic evaluation of a cysteine-bonded zwitterionic hydrophilic interaction liquid chromatography stationary phase".JOURNAL OF CHROMATOGRAPHY A 1228(2012):175-182.
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