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学科主题: 物理化学
题名: Preparation and chromatographic evaluation of a cysteine-bonded zwitterionic hydrophilic interaction liquid chromatography stationary phase
作者: Shen, Aijin1;  Guo, Zhimou1;  Cai, Xiaoming1;  Xue, Xingya1;  Liang, Xinmiao1
通讯作者: 梁鑫淼 ;  郭志谋
关键词: Hydrophilic interaction chromatography ;  "Thiol-ene" click chemistry ;  Zwitterionic stationary phase ;  Cysteine ;  2D-LC ;  Orthogonality
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2012-03-09
DOI: 10.1016/j.chroma.2011.10.086
卷: 1228, 页:175-182
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A cysteine-bonded zwitterionic hydrophilic interaction chromatography (HILIC) stationary phase (Click TE-Cys) was prepared based on the "thiol-ene" click chemistry. The Click TE-Cys material was characterized by solid state C-13 cross polarization/magic-angle spinning (CP/MAS) NMR and elemental analysis. The dynamic evaluation for cytosine, cytidine and orotic acid was performed using Van Deemter plots. The plate height values were no more than 24 mu m for the flow rate between 0.5 and 5.4 mm s(-1) (0.3-3.5 mL min(-1)), which proved the excellent separation efficiency of Click TE-Cys stationary phase. The influences of the content of water, concentration of salt and pH of the buffer solution on the retention of model compounds were investigated. The results demonstrated that the separation of polar analytes was dominated by the partitioning mechanism, while the contribution of electrostatic interaction was minor. The thermodynamic characteristic of Click TE-Cys stationary phase was also studied according to van't Hoff plot. An exothermic process for transferring analytes from the mobile phase to the stationary phase was observed and a linear relationship for Ink and 1/T was achieved, indicating no change of retention mechanism within the measured temperature range. Besides, the zwitterionic stationary phase exhibited good stability. Considering the high hydrophilicity of Click TE-Cys stationary phase, the application in the separation of protein tryptic digests was carried out using hydrophilic interaction chromatography-electrospray ionization mass spectrometry (HILIC-ESI-MS). More peaks were adequately resolved on the Click TE-Cys column comparing with that on the TSK Amide-80 column. In addition, the orthogonality between HILIC and RPLC system was investigated utilizing geometric approach. The XTerra MS C-18 and Click TE-Cys column displayed great difference in separation selectivity, with the orthogonality reaching 88.0%. On the other hand, the orthogonality between Click TE-Cys and TSK Amide-80 system was 21.4%, i.e. the selectivity was similar but slightly different from each other. The successful separation of protein digests indicated the great potential of Click TE-Cys stationary phase in the separation of complex samples and applicability in two-dimensional liquid chromatography (2D-LC). (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: MIXED TRIFUNCTIONAL SILANES ;  CLICK-CHEMISTRY ;  POLAR COMPOUNDS ;  MASS-SPECTROMETRY ;  SILICA-GEL ;  HORIZONTAL POLYMERIZATION ;  REVERSED-PHASE ;  HPLC SYSTEM ;  SEPARATION ;  RETENTION
语种: 英语
WOS记录号: WOS:000301406100017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117880
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Shen, Aijin,Guo, Zhimou,Cai, Xiaoming,et al. Preparation and chromatographic evaluation of a cysteine-bonded zwitterionic hydrophilic interaction liquid chromatography stationary phase[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1228:175-182.
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