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学科主题物理化学
Preparation and chromatographic evaluation of a cysteine-bonded zwitterionic hydrophilic interaction liquid chromatography stationary phase
Shen, Aijin; Guo, Zhimou; Cai, Xiaoming; Xue, Xingya; Liang, Xinmiao; Liang XM(梁鑫淼); Guo ZM(郭志谋)
关键词Hydrophilic Interaction Chromatography "thiol-ene" Click Chemistry Zwitterionic Stationary Phase Cysteine 2d-lc Orthogonality
刊名JOURNAL OF CHROMATOGRAPHY A
2012-03-09
DOI10.1016/j.chroma.2011.10.086
1228页:175-182
收录类别SCI
文章类型Article
部门归属18
项目归属1803
产权排名1,1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]MIXED TRIFUNCTIONAL SILANES ; CLICK-CHEMISTRY ; POLAR COMPOUNDS ; MASS-SPECTROMETRY ; SILICA-GEL ; HORIZONTAL POLYMERIZATION ; REVERSED-PHASE ; HPLC SYSTEM ; SEPARATION ; RETENTION
英文摘要A cysteine-bonded zwitterionic hydrophilic interaction chromatography (HILIC) stationary phase (Click TE-Cys) was prepared based on the "thiol-ene" click chemistry. The Click TE-Cys material was characterized by solid state C-13 cross polarization/magic-angle spinning (CP/MAS) NMR and elemental analysis. The dynamic evaluation for cytosine, cytidine and orotic acid was performed using Van Deemter plots. The plate height values were no more than 24 mu m for the flow rate between 0.5 and 5.4 mm s(-1) (0.3-3.5 mL min(-1)), which proved the excellent separation efficiency of Click TE-Cys stationary phase. The influences of the content of water, concentration of salt and pH of the buffer solution on the retention of model compounds were investigated. The results demonstrated that the separation of polar analytes was dominated by the partitioning mechanism, while the contribution of electrostatic interaction was minor. The thermodynamic characteristic of Click TE-Cys stationary phase was also studied according to van't Hoff plot. An exothermic process for transferring analytes from the mobile phase to the stationary phase was observed and a linear relationship for Ink and 1/T was achieved, indicating no change of retention mechanism within the measured temperature range. Besides, the zwitterionic stationary phase exhibited good stability. Considering the high hydrophilicity of Click TE-Cys stationary phase, the application in the separation of protein tryptic digests was carried out using hydrophilic interaction chromatography-electrospray ionization mass spectrometry (HILIC-ESI-MS). More peaks were adequately resolved on the Click TE-Cys column comparing with that on the TSK Amide-80 column. In addition, the orthogonality between HILIC and RPLC system was investigated utilizing geometric approach. The XTerra MS C-18 and Click TE-Cys column displayed great difference in separation selectivity, with the orthogonality reaching 88.0%. On the other hand, the orthogonality between Click TE-Cys and TSK Amide-80 system was 21.4%, i.e. the selectivity was similar but slightly different from each other. The successful separation of protein digests indicated the great potential of Click TE-Cys stationary phase in the separation of complex samples and applicability in two-dimensional liquid chromatography (2D-LC). (C) 2011 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000301406100017
引用统计
被引频次:74[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/117880
专题中国科学院大连化学物理研究所
通讯作者Liang XM(梁鑫淼); Guo ZM(郭志谋)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Shen, Aijin,Guo, Zhimou,Cai, Xiaoming,et al. Preparation and chromatographic evaluation of a cysteine-bonded zwitterionic hydrophilic interaction liquid chromatography stationary phase[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1228:175-182.
APA Shen, Aijin.,Guo, Zhimou.,Cai, Xiaoming.,Xue, Xingya.,Liang, Xinmiao.,...&郭志谋.(2012).Preparation and chromatographic evaluation of a cysteine-bonded zwitterionic hydrophilic interaction liquid chromatography stationary phase.JOURNAL OF CHROMATOGRAPHY A,1228,175-182.
MLA Shen, Aijin,et al."Preparation and chromatographic evaluation of a cysteine-bonded zwitterionic hydrophilic interaction liquid chromatography stationary phase".JOURNAL OF CHROMATOGRAPHY A 1228(2012):175-182.
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