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学科主题: 物理化学
题名: Mesoporous TiO2 aerogel for selective enrichment of phosphopeptides in rat liver mitochondria
作者: Zhang, Liyuan1, 2;  Liang, Zhen1;  Yang, Kaiguang1;  Xia, Simin1, 2;  Wu, Qi1, 2;  Zhang, Lihua1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: TiO2 aerogel ;  Phosphoproteome ;  Mass spectrometry ;  Nano-Reversed phase liquid chromatography-electrospray ionization tandem mass spectrometry ;  Rat liver mitochondria
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2012-06-04
DOI: 10.1016/j.aca.2012.04.005
卷: 729, 页:26-35
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1,1
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The enrichment of low abundance phosphopeptides before MS analysis is a critical step for in-depth phosphoproteome research. In this study, mesoporous titanium dioxide (TiO2) aerogel was prepared by precipitation and supercritical drying. The specific surface area up to 490.7 m(2) g(-1) is achieved by TiO2 aerogel, much higher than those obtained by commercial TiO2 nanoparticles and by the latest reported mesoporous TiO2 spheres. Due to the large specific surface area and the mesoporous structure of the aerogel, the binding capacity for phosphopeptides is six times higher than that of conventional TiO2 microparticles (173 vs 28 mu mol g(-1)). Because of the good compatibility of enrichment procedure with MALDI-TOF-MS and the large binding capacity of TiO2 aerogel, a detection limit as low as 30 amol for analyzing phosphopeptides in beta-casein digest was achieved. TiO2 aerogel was further applied to enrich phosphopeptides from rat liver mitochondria, and 266 unique phosphopeptides with 340 phosphorylation sites, corresponding to 216 phosphoprotein groups, were identified by triplicate nanoRPLC-ESI-MS/MS runs, with false-positive rate less than 1% at the peptide level. These results demonstrate that TiO2 aerogel is a kind of promising material for sample pretreatment in the large-scale phosphoproteome study. (C) 2012 Elsevier B.V. All rights reserved.
关键词[WOS]: ION AFFINITY-CHROMATOGRAPHY ;  IONIZATION MASS-SPECTROMETRY ;  TITANIUM-DIOXIDE ;  PHOSPHORYLATED PEPTIDES ;  QUANTITATIVE PHOSPHOPROTEOMICS ;  SILICA MICROSPHERES ;  METAL ;  EXTRACTION
语种: 英语
WOS记录号: WOS:000304681900004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117932
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Zhang, Liyuan,Liang, Zhen,Yang, Kaiguang,et al. Mesoporous TiO2 aerogel for selective enrichment of phosphopeptides in rat liver mitochondria[J]. ANALYTICA CHIMICA ACTA,2012,729:26-35.
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