DICP OpenIR
Subject Area物理化学
Mesoporous TiO2 aerogel for selective enrichment of phosphopeptides in rat liver mitochondria
Zhang, Liyuan1,2; Liang, Zhen1; Yang, Kaiguang1; Xia, Simin1,2; Wu, Qi1,2; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
KeywordTio2 Aerogel Phosphoproteome Mass Spectrometry Nano-reversed Phase Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry Rat Liver Mitochondria
Source PublicationANALYTICA CHIMICA ACTA
2012-06-04
DOI10.1016/j.aca.2012.04.005
Volume729Pages:26-35
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank1,1
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordION AFFINITY-CHROMATOGRAPHY ; IONIZATION MASS-SPECTROMETRY ; TITANIUM-DIOXIDE ; PHOSPHORYLATED PEPTIDES ; QUANTITATIVE PHOSPHOPROTEOMICS ; SILICA MICROSPHERES ; METAL ; EXTRACTION
AbstractThe enrichment of low abundance phosphopeptides before MS analysis is a critical step for in-depth phosphoproteome research. In this study, mesoporous titanium dioxide (TiO2) aerogel was prepared by precipitation and supercritical drying. The specific surface area up to 490.7 m(2) g(-1) is achieved by TiO2 aerogel, much higher than those obtained by commercial TiO2 nanoparticles and by the latest reported mesoporous TiO2 spheres. Due to the large specific surface area and the mesoporous structure of the aerogel, the binding capacity for phosphopeptides is six times higher than that of conventional TiO2 microparticles (173 vs 28 mu mol g(-1)). Because of the good compatibility of enrichment procedure with MALDI-TOF-MS and the large binding capacity of TiO2 aerogel, a detection limit as low as 30 amol for analyzing phosphopeptides in beta-casein digest was achieved. TiO2 aerogel was further applied to enrich phosphopeptides from rat liver mitochondria, and 266 unique phosphopeptides with 340 phosphorylation sites, corresponding to 216 phosphoprotein groups, were identified by triplicate nanoRPLC-ESI-MS/MS runs, with false-positive rate less than 1% at the peptide level. These results demonstrate that TiO2 aerogel is a kind of promising material for sample pretreatment in the large-scale phosphoproteome study. (C) 2012 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000304681900004
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/117932
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Affiliation1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Liyuan,Liang, Zhen,Yang, Kaiguang,et al. Mesoporous TiO2 aerogel for selective enrichment of phosphopeptides in rat liver mitochondria[J]. ANALYTICA CHIMICA ACTA,2012,729:26-35.
APA Zhang, Liyuan.,Liang, Zhen.,Yang, Kaiguang.,Xia, Simin.,Wu, Qi.,...&张丽华.(2012).Mesoporous TiO2 aerogel for selective enrichment of phosphopeptides in rat liver mitochondria.ANALYTICA CHIMICA ACTA,729,26-35.
MLA Zhang, Liyuan,et al."Mesoporous TiO2 aerogel for selective enrichment of phosphopeptides in rat liver mitochondria".ANALYTICA CHIMICA ACTA 729(2012):26-35.
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