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学科主题物理化学
Mesoporous TiO2 aerogel for selective enrichment of phosphopeptides in rat liver mitochondria
Zhang, Liyuan1,2; Liang, Zhen1; Yang, Kaiguang1; Xia, Simin1,2; Wu, Qi1,2; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
关键词Tio2 Aerogel Phosphoproteome Mass Spectrometry Nano-reversed Phase Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry Rat Liver Mitochondria
刊名ANALYTICA CHIMICA ACTA
2012-06-04
DOI10.1016/j.aca.2012.04.005
729页:26-35
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1,1
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]ION AFFINITY-CHROMATOGRAPHY ; IONIZATION MASS-SPECTROMETRY ; TITANIUM-DIOXIDE ; PHOSPHORYLATED PEPTIDES ; QUANTITATIVE PHOSPHOPROTEOMICS ; SILICA MICROSPHERES ; METAL ; EXTRACTION
英文摘要The enrichment of low abundance phosphopeptides before MS analysis is a critical step for in-depth phosphoproteome research. In this study, mesoporous titanium dioxide (TiO2) aerogel was prepared by precipitation and supercritical drying. The specific surface area up to 490.7 m(2) g(-1) is achieved by TiO2 aerogel, much higher than those obtained by commercial TiO2 nanoparticles and by the latest reported mesoporous TiO2 spheres. Due to the large specific surface area and the mesoporous structure of the aerogel, the binding capacity for phosphopeptides is six times higher than that of conventional TiO2 microparticles (173 vs 28 mu mol g(-1)). Because of the good compatibility of enrichment procedure with MALDI-TOF-MS and the large binding capacity of TiO2 aerogel, a detection limit as low as 30 amol for analyzing phosphopeptides in beta-casein digest was achieved. TiO2 aerogel was further applied to enrich phosphopeptides from rat liver mitochondria, and 266 unique phosphopeptides with 340 phosphorylation sites, corresponding to 216 phosphoprotein groups, were identified by triplicate nanoRPLC-ESI-MS/MS runs, with false-positive rate less than 1% at the peptide level. These results demonstrate that TiO2 aerogel is a kind of promising material for sample pretreatment in the large-scale phosphoproteome study. (C) 2012 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000304681900004
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/117932
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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Zhang, Liyuan,Liang, Zhen,Yang, Kaiguang,et al. Mesoporous TiO2 aerogel for selective enrichment of phosphopeptides in rat liver mitochondria[J]. ANALYTICA CHIMICA ACTA,2012,729:26-35.
APA Zhang, Liyuan.,Liang, Zhen.,Yang, Kaiguang.,Xia, Simin.,Wu, Qi.,...&张丽华.(2012).Mesoporous TiO2 aerogel for selective enrichment of phosphopeptides in rat liver mitochondria.ANALYTICA CHIMICA ACTA,729,26-35.
MLA Zhang, Liyuan,et al."Mesoporous TiO2 aerogel for selective enrichment of phosphopeptides in rat liver mitochondria".ANALYTICA CHIMICA ACTA 729(2012):26-35.
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