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学科主题物理化学
A new method for quantitative analysis of cell surface glycoproteome
Sun, Zhen; Chen, Rui; Cheng, Kai; Liu, Hongwei; Qin, Hongqiang; Ye, Mingliang; Zou, Hanfa; Zou HF(邹汉法)
关键词Cell Surface Glycoprotein Cd-annotated Protein Glycopeptides Glycoproteomics Non-glycopeptides Quantification
刊名PROTEOMICS
2012-11-01
ISSN1615-9853
DOI10.1002/pmic.201200150
12期:22页:3328-3337
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1,1
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]COLLAGENASE-STIMULATORY FACTOR ; MASS-SPECTROMETRY ; SITE-OCCUPANCY ; HYDRAZIDE CHEMISTRY ; MEMBRANE PROTEOME ; N-GLYCOSYLATION ; CHROMATOGRAPHY ; PROTEINS ; IDENTIFICATION ; QUANTIFICATION
英文摘要As the altered glycosylation expressions of cell surface proteins are associated with many diseases, glycoproteomics approach has been widely applied to characterization of surface glycosylation alteration. In general, the abundances of proteolytic glycopeptides derived from corresponding glycoproteins can be measured to determine the abundances of glycoproteins. However, this quantification strategy cannot distinguish whether the changes are results from changes of protein abundance or changes in glycosite occupancy. For the accurate and specific quantification of the cell surface glycosylation profile, we proposed a modified cell surface-capturing strategy where the glycopeptides were submitted to LC-MS/MS analysis directly for identification of glycoproteins and the non-glycopeptides were isotopically labelled for quantification of glycoproteins. This strategy was applied to comparatively analyze cell surface glycoproteins of two human cell lines, i.e. Chang Liver and HepG2 cells. Totally 341 glycoproteins were identified with 82.4% specificity for cell membrane proteins and 33 glycoproteins were quantified with significant expression change between the two cell lines. The differential expressions of two selected proteins (EMMPRIN and BCAM) were validated by Western blotting. This method enables specific and accurate analysis of the cell surface glycoproteins and may have broad application in the field of biomarker and drug target discovery.
语种英语
WOS记录号WOS:000311616000007
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/117934
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Sun, Zhen,Chen, Rui,Cheng, Kai,et al. A new method for quantitative analysis of cell surface glycoproteome[J]. PROTEOMICS,2012,12(22):3328-3337.
APA Sun, Zhen.,Chen, Rui.,Cheng, Kai.,Liu, Hongwei.,Qin, Hongqiang.,...&邹汉法.(2012).A new method for quantitative analysis of cell surface glycoproteome.PROTEOMICS,12(22),3328-3337.
MLA Sun, Zhen,et al."A new method for quantitative analysis of cell surface glycoproteome".PROTEOMICS 12.22(2012):3328-3337.
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