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学科主题: 物理化学
题名: A new method for quantitative analysis of cell surface glycoproteome
作者: Sun, Zhen1;  Chen, Rui1;  Cheng, Kai1;  Liu, Hongwei1;  Qin, Hongqiang1;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 邹汉法
关键词: Cell surface glycoprotein ;  CD-annotated protein ;  Glycopeptides ;  Glycoproteomics ;  Non-glycopeptides ;  Quantification
刊名: PROTEOMICS
发表日期: 2012-11-01
DOI: 10.1002/pmic.201200150
卷: 12, 期:22, 页:3328-3337
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1,1
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: As the altered glycosylation expressions of cell surface proteins are associated with many diseases, glycoproteomics approach has been widely applied to characterization of surface glycosylation alteration. In general, the abundances of proteolytic glycopeptides derived from corresponding glycoproteins can be measured to determine the abundances of glycoproteins. However, this quantification strategy cannot distinguish whether the changes are results from changes of protein abundance or changes in glycosite occupancy. For the accurate and specific quantification of the cell surface glycosylation profile, we proposed a modified cell surface-capturing strategy where the glycopeptides were submitted to LC-MS/MS analysis directly for identification of glycoproteins and the non-glycopeptides were isotopically labelled for quantification of glycoproteins. This strategy was applied to comparatively analyze cell surface glycoproteins of two human cell lines, i.e. Chang Liver and HepG2 cells. Totally 341 glycoproteins were identified with 82.4% specificity for cell membrane proteins and 33 glycoproteins were quantified with significant expression change between the two cell lines. The differential expressions of two selected proteins (EMMPRIN and BCAM) were validated by Western blotting. This method enables specific and accurate analysis of the cell surface glycoproteins and may have broad application in the field of biomarker and drug target discovery.
关键词[WOS]: COLLAGENASE-STIMULATORY FACTOR ;  MASS-SPECTROMETRY ;  SITE-OCCUPANCY ;  HYDRAZIDE CHEMISTRY ;  MEMBRANE PROTEOME ;  N-GLYCOSYLATION ;  CHROMATOGRAPHY ;  PROTEINS ;  IDENTIFICATION ;  QUANTIFICATION
语种: 英语
WOS记录号: WOS:000311616000007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117934
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Sun, Zhen,Chen, Rui,Cheng, Kai,et al. A new method for quantitative analysis of cell surface glycoproteome[J]. PROTEOMICS,2012,12(22):3328-3337.
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