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学科主题: 物理化学
题名: Signal-to-noise ratio enhancement of the compact light-emitting diode-induced fluorescence detector
作者: Geng, Xuhui1;  Wu, Dapeng1;  Wu, Qian1, 2;  Guan, Yafeng1
通讯作者: 关亚风
关键词: Light-emitting diode ;  Fluorescence detector ;  SNR enhancement ;  Compact
刊名: TALANTA
发表日期: 2012-10-15
DOI: 10.1016/j.talanta.2012.08.043
卷: 100, 页:27-31
收录类别: SCI
文章类型: Article
部门归属: 1
项目归属: 105
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: To enhance the SNR (signal-to-noise ratio) of the compact light-emitting diode-induced fluorescence detector (LED-FD) ([14]), key parameters including LED and its coupling style, pinhole diameter, calibration, and refractive index matching fluid (RIMF) were optimized. A 20 mA LED with light intensity of 8400 mcd was used as new light source. To optimize pinhole diameter, a theoretical analysis based on a three-dimensional (3D) view of the detection area was proposed and validated experimentally. A calibration principle from the 3D perspective was proposed. The detection flow cell and the collection fiber were adjusted to be coplanar. RIMF (glycerol) was applied to enhance SNR. The performance of the improved LED-FD was evaluated by flow injection analysis (HA). The limit of detection (LOD) was determined as 0.15 nM sodium fluorescein (SNR=3). Compared with our previous work, a five-fold enhancement on the SNR was obtained. The LOD was about five times higher than that of commercial Agilent G1321A FLD which employed a xenon pulse lamp. (C) 2012 Elsevier B.V. All rights reserved.
关键词[WOS]: CAPILLARY-FLOW SYSTEMS ;  ELECTROPHORESIS ;  PERFORMANCE
语种: 英语
WOS记录号: WOS:000313773300005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117962
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Key Lab Separat Sci Analyt Chem CAS, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Geng, Xuhui,Wu, Dapeng,Wu, Qian,et al. Signal-to-noise ratio enhancement of the compact light-emitting diode-induced fluorescence detector[J]. TALANTA,2012,100:27-31.
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