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学科主题物理化学
Short-chain peptides identification of scorpion Buthus martensi Karsch venom by employing high orthogonal 2D-HPLC system and tandem mass spectrometry
Xu, Junyan1,2; Zhang, Xiuli1; Guo, Zhimou1; Yan, Jingyu1; Yu, Long1; Li, Xiuling1; Xue, Xingya1; Liang, Xinmiao1; Liang XM(梁鑫淼)
关键词Hydrophilic Interaction Chromatography Peptide Separation Scorpion Venom Tandem Mass Spectrometry Two-dimensional Liquid Chromatography
刊名PROTEOMICS
2012-10-01
DOI10.1002/pmic.201200224
12期:19-20页:3076-3084
收录类别SCI
文章类型Article
部门归属18
项目归属1803
产权排名1,1
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]K+ CHANNEL ; PHARMACOLOGICAL CHARACTERIZATION ; LIQUID-CHROMATOGRAPHY ; POTASSIUM CHANNELS ; PROTEOMIC ANALYSIS ; RAT-BRAIN ; HILIC-RP ; PURIFICATION ; TOXINS ; SEPARATION
英文摘要Scorpion venom contains a considerable variety of neurotoxic peptides that can act on ionic channels. Here, we describe an orthogonal 2D-reversed phase/hydrophilic interaction chromatography system (RPLC/HILIC) and use it to separate short-chain peptides from Asian scorpion Buthus martensi Karsch (BmK) venom in a high throughput format. Due to its high orthogonality and efficiency, 18 homogenous peptides were purified and sequence identified by MS/MS with collision-induced dissociation. Among them, four peptides were discovered, which only have evidence at transcript-level, were first purified from crude venom in this study. Two peptides named BmKK2-b and Martentoxin-b were found the new cleaved chains of known BmKK2 and Martentoxin. In addition, two novel peptides named BmKK12 and BmKK16 in this paper were sequenced by de novo MS/MS, which we predict, are members of potassium channel toxin a-KTx 17 subfamily by homology to other known peptides found in the Swiss-Prot protein database.
语种英语
WOS记录号WOS:000310076300017
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/117976
专题中国科学院大连化学物理研究所
通讯作者Liang XM(梁鑫淼)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Liaoning, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
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GB/T 7714
Xu, Junyan,Zhang, Xiuli,Guo, Zhimou,et al. Short-chain peptides identification of scorpion Buthus martensi Karsch venom by employing high orthogonal 2D-HPLC system and tandem mass spectrometry[J]. PROTEOMICS,2012,12(19-20):3076-3084.
APA Xu, Junyan.,Zhang, Xiuli.,Guo, Zhimou.,Yan, Jingyu.,Yu, Long.,...&梁鑫淼.(2012).Short-chain peptides identification of scorpion Buthus martensi Karsch venom by employing high orthogonal 2D-HPLC system and tandem mass spectrometry.PROTEOMICS,12(19-20),3076-3084.
MLA Xu, Junyan,et al."Short-chain peptides identification of scorpion Buthus martensi Karsch venom by employing high orthogonal 2D-HPLC system and tandem mass spectrometry".PROTEOMICS 12.19-20(2012):3076-3084.
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