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学科主题: 物理化学
题名: Short-chain peptides identification of scorpion Buthus martensi Karsch venom by employing high orthogonal 2D-HPLC system and tandem mass spectrometry
作者: Xu, Junyan1, 2;  Zhang, Xiuli1;  Guo, Zhimou1;  Yan, Jingyu1;  Yu, Long1;  Li, Xiuling1;  Xue, Xingya1;  Liang, Xinmiao1
通讯作者: 梁鑫淼
关键词: Hydrophilic interaction chromatography ;  Peptide separation ;  Scorpion venom ;  Tandem mass spectrometry ;  Two-dimensional liquid chromatography
刊名: PROTEOMICS
发表日期: 2012-10-01
DOI: 10.1002/pmic.201200224
卷: 12, 期:19-20, 页:3076-3084
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1,1
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Scorpion venom contains a considerable variety of neurotoxic peptides that can act on ionic channels. Here, we describe an orthogonal 2D-reversed phase/hydrophilic interaction chromatography system (RPLC/HILIC) and use it to separate short-chain peptides from Asian scorpion Buthus martensi Karsch (BmK) venom in a high throughput format. Due to its high orthogonality and efficiency, 18 homogenous peptides were purified and sequence identified by MS/MS with collision-induced dissociation. Among them, four peptides were discovered, which only have evidence at transcript-level, were first purified from crude venom in this study. Two peptides named BmKK2-b and Martentoxin-b were found the new cleaved chains of known BmKK2 and Martentoxin. In addition, two novel peptides named BmKK12 and BmKK16 in this paper were sequenced by de novo MS/MS, which we predict, are members of potassium channel toxin a-KTx 17 subfamily by homology to other known peptides found in the Swiss-Prot protein database.
关键词[WOS]: K+ CHANNEL ;  PHARMACOLOGICAL CHARACTERIZATION ;  LIQUID-CHROMATOGRAPHY ;  POTASSIUM CHANNELS ;  PROTEOMIC ANALYSIS ;  RAT-BRAIN ;  HILIC-RP ;  PURIFICATION ;  TOXINS ;  SEPARATION
语种: 英语
WOS记录号: WOS:000310076300017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117976
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Liaoning, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Xu, Junyan,Zhang, Xiuli,Guo, Zhimou,et al. Short-chain peptides identification of scorpion Buthus martensi Karsch venom by employing high orthogonal 2D-HPLC system and tandem mass spectrometry[J]. PROTEOMICS,2012,12(19-20):3076-3084.
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