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学科主题: 物理化学
题名: Hydrophilic modification of silica-titania mesoporous materials as restricted-access matrix adsorbents for enrichment of phosphopeptides
作者: Wang, Fei1;  Guan, Yafeng2;  Zhang, Sen3;  Xia, Yan1
通讯作者: YanXia
关键词: Restricted-access matrix (RAM) adsorbents ;  Mesoporous materials (Ti-MCM-41) ;  Hydrophilic Modification ;  Phosphopeptide enrichment ;  Tryptic digestion
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2012-07-13
DOI: 10.1016/j.chroma.2012.02.050
卷: 1246, 页:76-83
收录类别: SCI ;  ISTP
文章类型: Article
部门归属: 1
项目归属: 105
产权排名: 2,2
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A new nano-scale restricted-access matrix (RAM) SiO2 (MCM-41) with relatively high Ti-content (Ti/Si = 0.1), but superior surface area (1129 m(2)/g), was successfully synthesised for the enrichment of phosphopeptides. The TiO2 was incorporated into the Si-MCM-41 via a hydrothermal process and the external surface was modified with alkyl diol by the successive hydrolysis of gamma-(glycidyloxypropyl) trimethoxysilane (GPTMS). Scanning electron microscopy, transmission electron microscopy, N-2 adsorption and Fourier transform infrared spectroscopy were used to characterise the alkyl diol-Ti-MCM-41. The appropriate pore diameter (2 nm) coupled to the marshy weeds-like hydrophilic external surface result in an efficient size-exclusion effect for the adsorption of standard cytochrome c with a molecular weight (MW) of ca. 12.4 kDa. At the same time, the strong affinity interaction between the incorporated titanium in the framework and the phosphoryl groups of phosphopeptides demonstrated a selective extract of phosphopeptides from the tryptic digestion. The detection sensitivity for phosphopeptides, determined by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) was as low as 5 fmol for standard tryptic digest of beta-casein. Therefore, this alkyl diol-Ti-MCM-41 mesoporous material can be used as a potential adsorbent for applications in MS-based phosphoproteomics. (C) 2012 Elsevier B.V. All rights reserved.
关键词[WOS]: ION AFFINITY-CHROMATOGRAPHY ;  PROTEIN-PHOSPHORYLATION ;  LIQUID-CHROMATOGRAPHY ;  SURFACE-AREA ;  CATALYSTS ;  COLUMN ;  EPOXIDATION ;  FUNCTIONALIZATION ;  PHOSPHOPROTEOME ;  PHOSPHATES
语种: 英语
WOS记录号: WOS:000306246000013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117979
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Res Ctr Analyt Sci, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Nankai Univ, State Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China

Recommended Citation:
Wang, Fei,Guan, Yafeng,Zhang, Sen,et al. Hydrophilic modification of silica-titania mesoporous materials as restricted-access matrix adsorbents for enrichment of phosphopeptides[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1246:76-83.
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