DICP OpenIR
Subject Area物理化学
Hydrophilic modification of silica-titania mesoporous materials as restricted-access matrix adsorbents for enrichment of phosphopeptides
Wang, Fei1; Guan, Yafeng2; Zhang, Sen3; Xia, Yan1; YanXia
KeywordRestricted-access Matrix (Ram) Adsorbents Mesoporous Materials (Ti-mcm-41) Hydrophilic Modification Phosphopeptide Enrichment Tryptic Digestion
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2012-07-13
DOI10.1016/j.chroma.2012.02.050
Volume1246Pages:76-83
Indexed BySCI ; ISTP
SubtypeArticle
Department1
Funding Project105
Contribution Rank2,2
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordION AFFINITY-CHROMATOGRAPHY ; PROTEIN-PHOSPHORYLATION ; LIQUID-CHROMATOGRAPHY ; SURFACE-AREA ; CATALYSTS ; COLUMN ; EPOXIDATION ; FUNCTIONALIZATION ; PHOSPHOPROTEOME ; PHOSPHATES
AbstractA new nano-scale restricted-access matrix (RAM) SiO2 (MCM-41) with relatively high Ti-content (Ti/Si = 0.1), but superior surface area (1129 m(2)/g), was successfully synthesised for the enrichment of phosphopeptides. The TiO2 was incorporated into the Si-MCM-41 via a hydrothermal process and the external surface was modified with alkyl diol by the successive hydrolysis of gamma-(glycidyloxypropyl) trimethoxysilane (GPTMS). Scanning electron microscopy, transmission electron microscopy, N-2 adsorption and Fourier transform infrared spectroscopy were used to characterise the alkyl diol-Ti-MCM-41. The appropriate pore diameter (2 nm) coupled to the marshy weeds-like hydrophilic external surface result in an efficient size-exclusion effect for the adsorption of standard cytochrome c with a molecular weight (MW) of ca. 12.4 kDa. At the same time, the strong affinity interaction between the incorporated titanium in the framework and the phosphoryl groups of phosphopeptides demonstrated a selective extract of phosphopeptides from the tryptic digestion. The detection sensitivity for phosphopeptides, determined by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) was as low as 5 fmol for standard tryptic digest of beta-casein. Therefore, this alkyl diol-Ti-MCM-41 mesoporous material can be used as a potential adsorbent for applications in MS-based phosphoproteomics. (C) 2012 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000306246000013
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/117979
Collection中国科学院大连化学物理研究所
Corresponding AuthorYanXia
Affiliation1.Nankai Univ, Res Ctr Analyt Sci, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Nankai Univ, State Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China
Recommended Citation
GB/T 7714
Wang, Fei,Guan, Yafeng,Zhang, Sen,et al. Hydrophilic modification of silica-titania mesoporous materials as restricted-access matrix adsorbents for enrichment of phosphopeptides[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1246:76-83.
APA Wang, Fei,Guan, Yafeng,Zhang, Sen,Xia, Yan,&YanXia.(2012).Hydrophilic modification of silica-titania mesoporous materials as restricted-access matrix adsorbents for enrichment of phosphopeptides.JOURNAL OF CHROMATOGRAPHY A,1246,76-83.
MLA Wang, Fei,et al."Hydrophilic modification of silica-titania mesoporous materials as restricted-access matrix adsorbents for enrichment of phosphopeptides".JOURNAL OF CHROMATOGRAPHY A 1246(2012):76-83.
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