DICP OpenIR
Subject Area物理化学
Highly Efficient Extraction of Cellular Nucleic Acid Associated Proteins in Vitro with Magnetic Oxidized Carbon Nanotubes
Zhang, Yi1,2; Hu, Zhengyan1,2; Qin, Hongqiang1,2; Wei, Xiaoluan1; Cheng, Kai1,2; Liu, Fangjie1,2; Wu, Ren'an1; Zou, Hanfa1; Wu RA(吴仁安); Zou HF(邹汉法)
Source PublicationANALYTICAL CHEMISTRY
2012-12-04
ISSN0003-2700
DOI10.1021/ac302695u
Volume84Issue:23Pages:10454-10462
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1,1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordDNA-BINDING PROTEINS ; SPECTROMETRY-BASED PROTEOMICS ; MASS-SPECTROMETRY ; ELECTROCHEMICAL DETECTION ; QUANTITATIVE PROTEOMICS ; SEQUENCES ; NETWORKS ; DISEASE ; LINKING
AbstractNucleic acid associated proteins (NAaP) play the essential roles in gene regulation and protein expression. The global analysis of cellular NAaP would give a broad insight to understand the interaction between nucleic acids and the associated proteins, such as the important proteinous regulation factors on nucleic acids. Proteomic analysis presents a novel strategy to investigate a group of proteins. However, the large scale analysis of NAaP is yet impossible due to the lack of approaches to harvest target protein groups with a high efficiency. Herein, a simple and efficient method was developed to collect cellular NAaP using magnetic oxidized carbon nanotubes based on the strong interaction between carbon nanotubes and nucleic acids along with corresponding associated proteins. We found that the magnetic oxidized carbon nanotubes demonstrated a nearly 100% extraction efficiency for intracellular nucleic acids from cells in vitro. Importantly, the proteins associated on nucleic acids could be highly efficiently harvested using magnetic oxidized carbon nanotubes due to the binding of NAaP on nucleic acids. 1594 groups of nuclear NAaP and 2595 groups of cellular NAaP were extracted and identified from about 1 000 000 cells, and 803 groups of NAaP were analyzed with only about 10 000 cells, showing a promising performance for the proteomic analysis of NAaP from minute cellular samples. This highly efficient extraction strategy for NAaP is a simple approach to identify cellular nucleic acid associated proteome, and we believed this strategy could be further applied in systems biology to understand the gene expression and regulation.
Language英语
WOS IDWOS:000311815300043
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/117982
Collection中国科学院大连化学物理研究所
Corresponding AuthorWu RA(吴仁安); Zou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Yi,Hu, Zhengyan,Qin, Hongqiang,et al. Highly Efficient Extraction of Cellular Nucleic Acid Associated Proteins in Vitro with Magnetic Oxidized Carbon Nanotubes[J]. ANALYTICAL CHEMISTRY,2012,84(23):10454-10462.
APA Zhang, Yi.,Hu, Zhengyan.,Qin, Hongqiang.,Wei, Xiaoluan.,Cheng, Kai.,...&邹汉法.(2012).Highly Efficient Extraction of Cellular Nucleic Acid Associated Proteins in Vitro with Magnetic Oxidized Carbon Nanotubes.ANALYTICAL CHEMISTRY,84(23),10454-10462.
MLA Zhang, Yi,et al."Highly Efficient Extraction of Cellular Nucleic Acid Associated Proteins in Vitro with Magnetic Oxidized Carbon Nanotubes".ANALYTICAL CHEMISTRY 84.23(2012):10454-10462.
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