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学科主题: 物理化学
题名: Highly Efficient Extraction of Cellular Nucleic Acid Associated Proteins in Vitro with Magnetic Oxidized Carbon Nanotubes
作者: Zhang, Yi1, 2;  Hu, Zhengyan1, 2;  Qin, Hongqiang1, 2;  Wei, Xiaoluan1;  Cheng, Kai1, 2;  Liu, Fangjie1, 2;  Wu, Ren'an1;  Zou, Hanfa1
通讯作者: 吴仁安 ;  邹汉法
刊名: ANALYTICAL CHEMISTRY
发表日期: 2012-12-04
DOI: 10.1021/ac302695u
卷: 84, 期:23, 页:10454-10462
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Nucleic acid associated proteins (NAaP) play the essential roles in gene regulation and protein expression. The global analysis of cellular NAaP would give a broad insight to understand the interaction between nucleic acids and the associated proteins, such as the important proteinous regulation factors on nucleic acids. Proteomic analysis presents a novel strategy to investigate a group of proteins. However, the large scale analysis of NAaP is yet impossible due to the lack of approaches to harvest target protein groups with a high efficiency. Herein, a simple and efficient method was developed to collect cellular NAaP using magnetic oxidized carbon nanotubes based on the strong interaction between carbon nanotubes and nucleic acids along with corresponding associated proteins. We found that the magnetic oxidized carbon nanotubes demonstrated a nearly 100% extraction efficiency for intracellular nucleic acids from cells in vitro. Importantly, the proteins associated on nucleic acids could be highly efficiently harvested using magnetic oxidized carbon nanotubes due to the binding of NAaP on nucleic acids. 1594 groups of nuclear NAaP and 2595 groups of cellular NAaP were extracted and identified from about 1 000 000 cells, and 803 groups of NAaP were analyzed with only about 10 000 cells, showing a promising performance for the proteomic analysis of NAaP from minute cellular samples. This highly efficient extraction strategy for NAaP is a simple approach to identify cellular nucleic acid associated proteome, and we believed this strategy could be further applied in systems biology to understand the gene expression and regulation.
关键词[WOS]: DNA-BINDING PROTEINS ;  SPECTROMETRY-BASED PROTEOMICS ;  MASS-SPECTROMETRY ;  ELECTROCHEMICAL DETECTION ;  QUANTITATIVE PROTEOMICS ;  SEQUENCES ;  NETWORKS ;  DISEASE ;  LINKING
语种: 英语
WOS记录号: WOS:000311815300043
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117982
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Zhang, Yi,Hu, Zhengyan,Qin, Hongqiang,et al. Highly Efficient Extraction of Cellular Nucleic Acid Associated Proteins in Vitro with Magnetic Oxidized Carbon Nanotubes[J]. ANALYTICAL CHEMISTRY,2012,84(23):10454-10462.
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