DICP OpenIR
学科主题物理化学
Highly Efficient Extraction of Cellular Nucleic Acid Associated Proteins in Vitro with Magnetic Oxidized Carbon Nanotubes
Zhang, Yi1,2; Hu, Zhengyan1,2; Qin, Hongqiang1,2; Wei, Xiaoluan1; Cheng, Kai1,2; Liu, Fangjie1,2; Wu, Ren'an1; Zou, Hanfa1; Wu RA(吴仁安); Zou HF(邹汉法)
刊名ANALYTICAL CHEMISTRY
2012-12-04
ISSN0003-2700
DOI10.1021/ac302695u
84期:23页:10454-10462
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1,1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]DNA-BINDING PROTEINS ; SPECTROMETRY-BASED PROTEOMICS ; MASS-SPECTROMETRY ; ELECTROCHEMICAL DETECTION ; QUANTITATIVE PROTEOMICS ; SEQUENCES ; NETWORKS ; DISEASE ; LINKING
英文摘要Nucleic acid associated proteins (NAaP) play the essential roles in gene regulation and protein expression. The global analysis of cellular NAaP would give a broad insight to understand the interaction between nucleic acids and the associated proteins, such as the important proteinous regulation factors on nucleic acids. Proteomic analysis presents a novel strategy to investigate a group of proteins. However, the large scale analysis of NAaP is yet impossible due to the lack of approaches to harvest target protein groups with a high efficiency. Herein, a simple and efficient method was developed to collect cellular NAaP using magnetic oxidized carbon nanotubes based on the strong interaction between carbon nanotubes and nucleic acids along with corresponding associated proteins. We found that the magnetic oxidized carbon nanotubes demonstrated a nearly 100% extraction efficiency for intracellular nucleic acids from cells in vitro. Importantly, the proteins associated on nucleic acids could be highly efficiently harvested using magnetic oxidized carbon nanotubes due to the binding of NAaP on nucleic acids. 1594 groups of nuclear NAaP and 2595 groups of cellular NAaP were extracted and identified from about 1 000 000 cells, and 803 groups of NAaP were analyzed with only about 10 000 cells, showing a promising performance for the proteomic analysis of NAaP from minute cellular samples. This highly efficient extraction strategy for NAaP is a simple approach to identify cellular nucleic acid associated proteome, and we believed this strategy could be further applied in systems biology to understand the gene expression and regulation.
语种英语
WOS记录号WOS:000311815300043
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/117982
专题中国科学院大连化学物理研究所
通讯作者Wu RA(吴仁安); Zou HF(邹汉法)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Yi,Hu, Zhengyan,Qin, Hongqiang,et al. Highly Efficient Extraction of Cellular Nucleic Acid Associated Proteins in Vitro with Magnetic Oxidized Carbon Nanotubes[J]. ANALYTICAL CHEMISTRY,2012,84(23):10454-10462.
APA Zhang, Yi.,Hu, Zhengyan.,Qin, Hongqiang.,Wei, Xiaoluan.,Cheng, Kai.,...&邹汉法.(2012).Highly Efficient Extraction of Cellular Nucleic Acid Associated Proteins in Vitro with Magnetic Oxidized Carbon Nanotubes.ANALYTICAL CHEMISTRY,84(23),10454-10462.
MLA Zhang, Yi,et al."Highly Efficient Extraction of Cellular Nucleic Acid Associated Proteins in Vitro with Magnetic Oxidized Carbon Nanotubes".ANALYTICAL CHEMISTRY 84.23(2012):10454-10462.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
2012OWwQlpCp97.PDF(378KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Yi]的文章
[Hu, Zhengyan]的文章
[Qin, Hongqiang]的文章
百度学术
百度学术中相似的文章
[Zhang, Yi]的文章
[Hu, Zhengyan]的文章
[Qin, Hongqiang]的文章
必应学术
必应学术中相似的文章
[Zhang, Yi]的文章
[Hu, Zhengyan]的文章
[Qin, Hongqiang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。