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学科主题物理化学
A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells
Yu, Fabiao1,2; Song, Ping1; Li, Peng1; Wang, Bingshuai1; Han, Keli1; Han KL(韩克利)
刊名ANALYST
2012
DOI10.1039/c2an35246j
137期:16页:3740-3749
收录类别SCI
文章类型Article
部门归属11
项目归属1101
产权排名1,1
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]NITRIC-OXIDE ; REACTIVE OXYGEN ; LUMINOL CHEMILUMINESCENCE ; BIOLOGICAL-SYSTEMS ; HYDROGEN-PEROXIDE ; MODEL SYSTEMS ; OFF-ON ; ACID ; SUPEROXIDE ; KINETICS
英文摘要We present the design, synthesis, spectroscopy, and biological applications of PyBor, a new type of fluorescent probe for peroxynitrite detection in aqueous solution and living cells. The probe employs pyrene as the fluorophore, and is equipped with a chemically responsive unit boronate. The fluorescent probe can selectively detect peroxynitrite with fluorimetric determination and high-performance liquid chromatography analyses in aqueous solution and RAW264.7 cells intracellular free extracts. We also study our probe to time dependent peroxynitrite release from 3-morpholinylsydnonimine hydrochloride. Confocal microscopy experiments using mouse macrophage cell line RAW264.7 show that PyBor is able to detect the different intracellular peroxynitrite levels. In addition, we have performed quantum chemical calculations with TD-DFT/M06/TZVP level with COSMO solvation model basis sets using a suite of Gaussian 09 programs to provide insights into the structure optical properties of PyBor and PyOH.
语种英语
WOS记录号WOS:000306483000021
引用统计
被引频次:58[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/117989
专题中国科学院大连化学物理研究所
通讯作者Han KL(韩克利)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Sch Chem Engn, Dalian 116024, Peoples R China
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GB/T 7714
Yu, Fabiao,Song, Ping,Li, Peng,et al. A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells[J]. ANALYST,2012,137(16):3740-3749.
APA Yu, Fabiao,Song, Ping,Li, Peng,Wang, Bingshuai,Han, Keli,&韩克利.(2012).A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells.ANALYST,137(16),3740-3749.
MLA Yu, Fabiao,et al."A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells".ANALYST 137.16(2012):3740-3749.
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