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学科主题: 物理化学
题名: A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells
作者: Yu, Fabiao1, 2;  Song, Ping1;  Li, Peng1;  Wang, Bingshuai1;  Han, Keli1
通讯作者: 韩克利
刊名: ANALYST
发表日期: 2012
DOI: 10.1039/c2an35246j
卷: 137, 期:16, 页:3740-3749
收录类别: SCI
文章类型: Article
部门归属: 11
项目归属: 1101
产权排名: 1,1
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: We present the design, synthesis, spectroscopy, and biological applications of PyBor, a new type of fluorescent probe for peroxynitrite detection in aqueous solution and living cells. The probe employs pyrene as the fluorophore, and is equipped with a chemically responsive unit boronate. The fluorescent probe can selectively detect peroxynitrite with fluorimetric determination and high-performance liquid chromatography analyses in aqueous solution and RAW264.7 cells intracellular free extracts. We also study our probe to time dependent peroxynitrite release from 3-morpholinylsydnonimine hydrochloride. Confocal microscopy experiments using mouse macrophage cell line RAW264.7 show that PyBor is able to detect the different intracellular peroxynitrite levels. In addition, we have performed quantum chemical calculations with TD-DFT/M06/TZVP level with COSMO solvation model basis sets using a suite of Gaussian 09 programs to provide insights into the structure optical properties of PyBor and PyOH.
关键词[WOS]: NITRIC-OXIDE ;  REACTIVE OXYGEN ;  LUMINOL CHEMILUMINESCENCE ;  BIOLOGICAL-SYSTEMS ;  HYDROGEN-PEROXIDE ;  MODEL SYSTEMS ;  OFF-ON ;  ACID ;  SUPEROXIDE ;  KINETICS
语种: 英语
WOS记录号: WOS:000306483000021
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117989
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Sch Chem Engn, Dalian 116024, Peoples R China

Recommended Citation:
Yu, Fabiao,Song, Ping,Li, Peng,et al. A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells[J]. ANALYST,2012,137(16):3740-3749.
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