中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Hydrophilic immobilized trypsin reactor with magnetic graphene oxide as support for high efficient proteome digestion
作者: Jiang, Bo1, 2, 3;  Yang, Kaiguang1;  Zhao, Qun1, 2;  Wu, Qi1, 2;  Liang, Zhen1;  Zhang, Lihua1;  Peng, Xiaojun3;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Immobilized enzymatic reactor ;  Graphene oxide ;  Magnetic nanoparticles ;  Proteomics
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2012-09-07
DOI: 10.1016/j.chroma.2012.07.030
卷: 1254, 页:8-13
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: In this paper, magnetic Fe3O4 nanoparticles modified graphene oxide nanocomposites (GO-CO-NH-Fe3O4) were prepared by covalent bonding, via the reaction between the amino groups of fuctionalized Fe3O4 and the carboxylic groups of GO, confirmed by Fourier-transform infrared spectra, Raman spectroscopy, and transmission electron microscopy. With GO-CO-NH-Fe3O4 as a novel substrate, trypsin was immobilized via pi-pi stacking and hydrogen bonding interaction, and the binding capacity of trypsin reached as high as 0.275 mg/mg. Since GO-CO-NH-Fe3O4 worked as not only support for enzyme immobilization, but also as an excellent microwave irradiation absorber, the digestion efficiency could be further improved with microwave assistance. By such an immobilized enzymatic reactor (IMER), standard proteins could be efficiently digested within 15 s, with sequence coverages comparable or better than those obtained by conventional in-solution digestion (12 h). Since trypsin was immobilized under mild conditions, the enzymatic activity of IMER preserved at least for a month. In addition, due to the good hydrophilicity of GO, no peptide residue was observed in the sequent digestion of bovine serum albumin and myoglobin. To further confirm the efficiency of such an IMER for proteome analysis, it was applied to digest proteins extracted from rat liver, followed by nanoRPLC-ESI-MS/MS analysis. With only 5 min microwave-assisted digestion, in 3 parallel runs, totally 456 protein groups were identified, comparable to that obtained by 12 h in-solution digestion, indicating the great potential of IMERs with GO-CO-NH-Fe3O4 as the support for high throughput proteome study. (c) 2012 Elsevier B.V. All rights reserved.
关键词[WOS]: ON-PLATE PROTEOLYSIS ;  MASS-SPECTROMETRY ;  MS ANALYSIS ;  SILICA ;  NANOPARTICLES ;  ONLINE ;  MICROREACTOR ;  FABRICATION ;  MONOLITHS ;  SHEETS
语种: 英语
WOS记录号: WOS:000308048000002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/117993
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
2012OkDDUQVoZ0.PDF(734KB)----开放获取--View 联系获取全文

作者单位: 1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China

Recommended Citation:
Jiang, Bo,Yang, Kaiguang,Zhao, Qun,et al. Hydrophilic immobilized trypsin reactor with magnetic graphene oxide as support for high efficient proteome digestion[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1254:8-13.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Jiang, Bo]'s Articles
 [Yang, Kaiguang]'s Articles
 [Zhao, Qun]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Jiang, Bo]‘s Articles
 [Yang, Kaiguang]‘s Articles
 [Zhao, Qun]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2012OkDDUQVoZ0.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace