DICP OpenIR
Subject Area物理化学
A microfluidic-based device for study of transendothelial invasion of tumor aggregates in realtime
Zhang, Qian1; Liu, Tingjiao1; Qin, Jianhua2; TingjiaoLiu; Qin JH(秦建华)
Source PublicationLAB ON A CHIP
2012
DOI10.1039/c2lc00030j
Volume12Issue:16Pages:2837-2842
Indexed BySCI
SubtypeArticle
Department18
Funding Project1807
Contribution Rank2,3
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS KeywordADENOID CYSTIC CARCINOMA ; CANCER METASTASIS ; IN-VITRO ; CHEMOKINE RECEPTORS ; CELLS ; MIGRATION ; CXCR4 ; CHEMOTHERAPY ; ENDOTHELIUM ; ADHESION
AbstractCirculating tumor aggregates exhibit a high metastatic potential and could potentially serve as an important target for cancer therapies. In this study, we developed a microfluidic model that reconstitutes and is representative of the principal components of biological blood vessels, including vessel cavity, endothelium, and perivascular matrix containing chemokines. Using this model, the transendothelial invasion of tumor aggregates can be observed and recorded in realtime. In this study we analyzed the extravasation process of salivary gland adenoid cystic carcinoma (ACC) cell aggregates. ACC aggregates transmigrated across the endothelium under the stimulation of chemokine CXCL12. The endothelial integrity was irreversibly damaged at the site of transendothelial invasion. The transendothelial invasion of ACC aggregates was inhibited by AMD3100, but the adhesion of ACC aggregates to the endothelium was not affected by the CXCR4 antagonist. This model allows for detailed study of the attachment and transendothelial invasion of tumor aggregates; thus, it would be a useful tool for analysis of the underlying mechanisms of metastasis and for testing novel anti-metastasis agents.
Language英语
WOS IDWOS:000306523800012
Citation statistics
Cited Times:73[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/117998
Collection中国科学院大连化学物理研究所
Corresponding AuthorTingjiaoLiu; Qin JH(秦建华)
Affiliation1.Dalian Med Univ, Coll Stomatol, Sect Oral Pathol, Dalian, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Beijing 100864, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Qian,Liu, Tingjiao,Qin, Jianhua,et al. A microfluidic-based device for study of transendothelial invasion of tumor aggregates in realtime[J]. LAB ON A CHIP,2012,12(16):2837-2842.
APA Zhang, Qian,Liu, Tingjiao,Qin, Jianhua,TingjiaoLiu,&秦建华.(2012).A microfluidic-based device for study of transendothelial invasion of tumor aggregates in realtime.LAB ON A CHIP,12(16),2837-2842.
MLA Zhang, Qian,et al."A microfluidic-based device for study of transendothelial invasion of tumor aggregates in realtime".LAB ON A CHIP 12.16(2012):2837-2842.
Files in This Item:
File Name/Size DocType Version Access License
2012OEq8gGltET.PDF(721KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Qian]'s Articles
[Liu, Tingjiao]'s Articles
[Qin, Jianhua]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Qian]'s Articles
[Liu, Tingjiao]'s Articles
[Qin, Jianhua]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Qian]'s Articles
[Liu, Tingjiao]'s Articles
[Qin, Jianhua]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.