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学科主题: 物理化学
题名: Capture and Dimethyl Labeling of Glycopeptides on Hydrazide Beads for Quantitative Glycoproteomics Analysis
作者: Sun, Zhen1;  Qin, Hongqiang1;  Wang, Fangjun1;  Cheng, Kai1;  Dong, Mingming1;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 邹汉法
刊名: ANALYTICAL CHEMISTRY
发表日期: 2012-10-16
DOI: 10.1021/ac302130r
卷: 84, 期:20, 页:8452-8456
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1, 1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Incorporation of isotopic tag onto peptides via chemical labeling is a popular approach for quantitative proteomics. Chemical labeling via solution based methods usually lead to a tedious process and sample loss because several sample preparation steps including buffer exchange and desalting are performed. In this study, a solid phase based labeling approach by integration of glycopeptide enrichment and stable isotope labeling on hydrazide beads was developed for relative quantification of protein glycosylation, by which enrichment, washing, labeling, and release of the glycopeptides were all performed on the hydrazide beads sequentially. This approach was proved to be accurate in quantitative glycoproteome analysis and have good linearity range with 2 orders of magnitude for quantification of glycopeptides. Compared with dimethyl labeling conventionally performed in solution, the developed approach has better enrichment recovery (10-330% improvement) and high detection sensitivity in which 4296 of annotated glycosites (vs 26%) still can be quantified using only 10 mu g of four standard glycoprotein mixtures and 400 mu g of bovine serum album interference as starting sample. The applicability of the approach for quantitative glycopeptide profiling was also explored by differential analysis of glycoproteome between human normal serum and liver cancer serum.
关键词[WOS]: CODED AFFINITY TAGS ;  MASS-SPECTROMETRY ;  PROTEOME ANALYSIS ;  ISOTOPE ;  CHROMATOGRAPHY ;  PEPTIDES
语种: 英语
WOS记录号: WOS:000309805200005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118001
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Sun, Zhen,Qin, Hongqiang,Wang, Fangjun,et al. Capture and Dimethyl Labeling of Glycopeptides on Hydrazide Beads for Quantitative Glycoproteomics Analysis[J]. ANALYTICAL CHEMISTRY,2012,84(20):8452-8456.
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