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学科主题物理化学
Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes
Wang, Fangjun; Zhu, Jun; Hu, Lianghai; Qin, Hongqiang; Ye, Mingliang; Zou, Hanfa; Zou HF(邹汉法)
关键词Human Serum Endogenous Peptide n And c Termini Disease Diagnosis
刊名PROTEIN & CELL
2012-09-01
ISSN1674-800X
DOI10.1007/s13238-012-2934-4
3期:9页:669-674
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1,1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]MASS-SPECTROMETRY ; PROTEOME ; PLASMA ; QUANTIFICATION ; PEPTIDOMICS
英文摘要The human serum proteome is closely associated with the state of the body. Endogenous peptides derived from proteolytic enzymes cleaving on serum proteins are widely studied due to their potential application in disease-specific marker discovery. However, the reproducibility of peptidome analysis of endogenous peptides is significantly influenced by the proteolytic enzymes within body fluids, thereby limiting the clinical use of the endogenous peptides. We comprehensively investigated the N and C terminus of endogenous peptides using peptidomics. The cleavage site patterns of the N and C terminus and adjacent sites from all the identified endogenous peptides were highly conserved under different sample preparation conditions, including long-term incubation at 37 degrees C and pretreatment with repeated freeze-thaw cycles. Furthermore, a distinguishable cleavage site pattern was obtained when a different disease serum was analyzed. The conserved cleavage site pattern derived from proteolytic enzymes holds potential in highly specific disease diagnosis.
语种英语
WOS记录号WOS:000310532800004
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/118013
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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GB/T 7714
Wang, Fangjun,Zhu, Jun,Hu, Lianghai,et al. Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes[J]. PROTEIN & CELL,2012,3(9):669-674.
APA Wang, Fangjun.,Zhu, Jun.,Hu, Lianghai.,Qin, Hongqiang.,Ye, Mingliang.,...&邹汉法.(2012).Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes.PROTEIN & CELL,3(9),669-674.
MLA Wang, Fangjun,et al."Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes".PROTEIN & CELL 3.9(2012):669-674.
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