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学科主题: 物理化学
题名: Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes
作者: Wang, Fangjun1;  Zhu, Jun1;  Hu, Lianghai1;  Qin, Hongqiang1;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 邹汉法
关键词: human serum ;  endogenous peptide ;  N and C termini ;  disease diagnosis
刊名: PROTEIN & CELL
发表日期: 2012-09-01
DOI: 10.1007/s13238-012-2934-4
卷: 3, 期:9, 页:669-674
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
英文摘要: The human serum proteome is closely associated with the state of the body. Endogenous peptides derived from proteolytic enzymes cleaving on serum proteins are widely studied due to their potential application in disease-specific marker discovery. However, the reproducibility of peptidome analysis of endogenous peptides is significantly influenced by the proteolytic enzymes within body fluids, thereby limiting the clinical use of the endogenous peptides. We comprehensively investigated the N and C terminus of endogenous peptides using peptidomics. The cleavage site patterns of the N and C terminus and adjacent sites from all the identified endogenous peptides were highly conserved under different sample preparation conditions, including long-term incubation at 37 degrees C and pretreatment with repeated freeze-thaw cycles. Furthermore, a distinguishable cleavage site pattern was obtained when a different disease serum was analyzed. The conserved cleavage site pattern derived from proteolytic enzymes holds potential in highly specific disease diagnosis.
关键词[WOS]: MASS-SPECTROMETRY ;  PROTEOME ;  PLASMA ;  QUANTIFICATION ;  PEPTIDOMICS
语种: 英语
WOS记录号: WOS:000310532800004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118013
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Fangjun,Zhu, Jun,Hu, Lianghai,et al. Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes[J]. PROTEIN & CELL,2012,3(9):669-674.
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