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学科主题: 物理化学
题名: Development of a combined chemical and enzymatic approach for the mass spectrometric identification and quantification of aberrant N-glycosylation
作者: Chen, Rui1;  Wang, Fangjun1;  Tan, Yexiong2;  Sun, Zhen1;  Song, Chunxia1;  Ye, Mingliang1;  Wang, Hongyang2;  Zou, Hanfa1
通讯作者: 邹汉法
关键词: Aberrant glycosylation ;  Mass spectrometry ;  Quantitative proteomics
刊名: JOURNAL OF PROTEOMICS
发表日期: 2012-02-16
DOI: 10.1016/j.jprot.2011.12.015
卷: 75, 期:5, 页:1666-1674
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Direct mass spectrometric analysis of aberrant protein glycosylation is a challenge to the current analytical techniques. Except lectin affinity chromatography, no other glycosylation enrichment techniques are available for analysis of aberrant glycosylation. In this study, we developed a combined chemical and enzymatic strategy as an alternative for the mass spectrometric analysis of aberrant glycosylation. Sialylated glycopeptides were enriched with reverse glycoblotting, cleaved by endoglycosidase F3 and analyzed by mass spectrometry with both neutral loss triggered MS3 in collision induced dissociation (CID) and electron transfer dissociation (ETD). Interestingly, a great part of resulted glycopeptides were found with fucose attached to the N-acetylglucosamine (N-GlcNAc), which indicated that the aberrant glycosylaton that is carrying both terminal sialylation and core fucosylation was identified. Totally, 69 aberrant N-glycosylation sites were identified in sera samples from hepatocellular carcinoma (HCC) patients. Following the identification, quantification of the level of this aberrant glycosylation was also carried out using stable isotope dimethyl labeling and pooled sera sample from liver cirrhosis and HCC was compared. Six glycosylation sites demonstrated elevated level of aberrancy, which demonstrated that our developed strategy was effective in both qualitative and quantitative studies of aberrant glycosylation. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: ELECTRON-TRANSFER DISSOCIATION ;  HEPATOCELLULAR-CARCINOMA ;  TARGETED GLYCOPROTEOMICS ;  CAPTURE DISSOCIATION ;  SERUM GLYCOPROTEINS ;  PEPTIDE ;  CHROMATOGRAPHY ;  FRAGMENTATION ;  GLYCOPEPTIDES ;  FUCOSYLATION
语种: 英语
WOS记录号: WOS:000300924700016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118109
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Second Mil Med Univ, Eastern Hepatobiliary Surg Inst, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China

Recommended Citation:
Chen, Rui,Wang, Fangjun,Tan, Yexiong,et al. Development of a combined chemical and enzymatic approach for the mass spectrometric identification and quantification of aberrant N-glycosylation[J]. JOURNAL OF PROTEOMICS,2012,75(5):1666-1674.
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