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学科主题: 物理化学
题名: Preparation and characterization of DNA aptamer based spin column for enrichment and separation of histones
作者: Shao, Nan1, 2;  Zhang, Kai1, 2;  Chen, Ying2;  He, Xiwen2;  Zhang, Yukui1, 2, 3
通讯作者: 何锡文
刊名: CHEMICAL COMMUNICATIONS
发表日期: 2012
DOI: 10.1039/c2cc32376a
卷: 48, 期:53, 页:6684-6686
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 3,5
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Core histones play a critical role in the regulation of chromatin-templated biological process. Here we have developed a novel approach to enrich histone proteins based on the recognition of a specific aptamer. The method shows good selectivity toward histone proteins and is able to extract histones directly from complex whole cell lysates.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  CHROMATIN ;  SELECTION ;  LIGANDS ;  H4
语种: 英语
WOS记录号: WOS:000305001700020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118146
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Shao, Nan,Zhang, Kai,Chen, Ying,et al. Preparation and characterization of DNA aptamer based spin column for enrichment and separation of histones[J]. CHEMICAL COMMUNICATIONS,2012,48(53):6684-6686.
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