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学科主题: 物理化学
题名: Strong cation exchange column allow for symmetrical peak shape and increased sample loading in the separation of basic compounds
作者: Long, Zhen1;  Wang, Chaoran1;  Guo, Zhimou1;  Zhang, Xiuli1;  Nordahl, Lilly1;  Liang, Xinmiao1
通讯作者: 梁鑫淼 ;  郭志谋
关键词: SCX ;  Basic compounds ;  Symmetrical peaks ;  Load amount ;  Separation efficiency
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2012-09-21
DOI: 10.1016/j.chroma.2012.07.008
卷: 1256, 页:67-71
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Strong cation exchange (SCX) mode, an alternative to reversed-phase (RP) mode, was described in this paper to obtain symmetrical peak shape and high sample loading for the separation of basic compounds. The retention mechanisms on SCX modified silica sorbents have been demonstrated including electrostatic and hydrophobic interactions. The effects of eluent ionic strength, apparent pH on retention were investigated and the cation-exchange capacity was also characterized. Good efficiency (>56,000 plate m(-1)) and excellent peak shape (Tf < 1.2) for various basic probes were obtained on the SCX column. Furthermore, good peak shapes and separation can be maintained under high concentration injections, indicating good potential in preparative use. These results were also compared with those obtained on two conventional RP stationary phases. The overall results demonstrated that SCX stationary phases can be used as alternatives to RP stationary phases in the separation of basic compounds, especially in the purification of basic compounds. (c) 2012 Elsevier B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  REVERSED-PHASE CHROMATOGRAPHY ;  ION CHROMATOGRAPHY ;  STATIONARY PHASES ;  DRUGS ;  PH ;  RETENTION ;  SYSTEM ;  ENRICHMENT ;  ALKALOIDS
语种: 英语
WOS记录号: WOS:000308624000009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118161
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning Provin, Peoples R China

Recommended Citation:
Long, Zhen,Wang, Chaoran,Guo, Zhimou,et al. Strong cation exchange column allow for symmetrical peak shape and increased sample loading in the separation of basic compounds[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1256:67-71.
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