DICP OpenIR
Subject Area物理化学
Strong cation exchange column allow for symmetrical peak shape and increased sample loading in the separation of basic compounds
Long, Zhen; Wang, Chaoran; Guo, Zhimou; Zhang, Xiuli; Nordahl, Lilly; Liang, Xinmiao; Liang XM(梁鑫淼); Guo ZM(郭志谋)
KeywordScx Basic Compounds Symmetrical Peaks Load Amount Separation Efficiency
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2012-09-21
DOI10.1016/j.chroma.2012.07.008
Volume1256Pages:67-71
Indexed BySCI
SubtypeArticle
Department18
Funding Project1803
Contribution Rank1,1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; REVERSED-PHASE CHROMATOGRAPHY ; ION CHROMATOGRAPHY ; STATIONARY PHASES ; DRUGS ; PH ; RETENTION ; SYSTEM ; ENRICHMENT ; ALKALOIDS
AbstractStrong cation exchange (SCX) mode, an alternative to reversed-phase (RP) mode, was described in this paper to obtain symmetrical peak shape and high sample loading for the separation of basic compounds. The retention mechanisms on SCX modified silica sorbents have been demonstrated including electrostatic and hydrophobic interactions. The effects of eluent ionic strength, apparent pH on retention were investigated and the cation-exchange capacity was also characterized. Good efficiency (>56,000 plate m(-1)) and excellent peak shape (Tf < 1.2) for various basic probes were obtained on the SCX column. Furthermore, good peak shapes and separation can be maintained under high concentration injections, indicating good potential in preparative use. These results were also compared with those obtained on two conventional RP stationary phases. The overall results demonstrated that SCX stationary phases can be used as alternatives to RP stationary phases in the separation of basic compounds, especially in the purification of basic compounds. (c) 2012 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000308624000009
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/118161
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiang XM(梁鑫淼); Guo ZM(郭志谋)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning Provin, Peoples R China
Recommended Citation
GB/T 7714
Long, Zhen,Wang, Chaoran,Guo, Zhimou,et al. Strong cation exchange column allow for symmetrical peak shape and increased sample loading in the separation of basic compounds[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1256:67-71.
APA Long, Zhen.,Wang, Chaoran.,Guo, Zhimou.,Zhang, Xiuli.,Nordahl, Lilly.,...&郭志谋.(2012).Strong cation exchange column allow for symmetrical peak shape and increased sample loading in the separation of basic compounds.JOURNAL OF CHROMATOGRAPHY A,1256,67-71.
MLA Long, Zhen,et al."Strong cation exchange column allow for symmetrical peak shape and increased sample loading in the separation of basic compounds".JOURNAL OF CHROMATOGRAPHY A 1256(2012):67-71.
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