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学科主题物理化学
A self-assembled polydopamine film on the surface of magnetic nanoparticles for specific capture of protein
Zhang, Min1,2; Zhang, Xihao1,2; He, Xiwen1,2; Chen, Langxing1,2; Zhang, Yukui1,2,3; Chen LX(陈朗星)
刊名NANOSCALE
2012
DOI10.1039/c2nr30316g
4期:10页:3141-3147
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名3,5
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
研究领域[WOS]Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
关键词[WOS]MOLECULARLY IMPRINTED POLYMERS ; HISTIDINE-TAGGED PROTEINS ; IRON-OXIDE NANOPARTICLES ; BOVINE HEMOGLOBIN ; BIOMEDICAL APPLICATIONS ; SORBENT ASSAYS ; AQUEOUS-MEDIA ; HUMAN SERUM ; RECOGNITION ; SHELL
英文摘要In this study, we report a facile method for the preparation of core-shell magnetic molecularly imprinted polymers (MIPs) for protein recognition. Uniform carboxyl group functionalized Fe3O4 nanoparticles (NPs) were synthesized using a solvothermal method. Magnetic MIPs were synthesized by self-polymerization of dopamine in the presence of template protein on the surface of the Fe3O4 NPs. A thin layer of polydopamine can be coated on Fe3O4 NPs via dopamine self-polymerization and the imprinted polydopamine shells can be controlled by the mass ratio of Fe3O4 NPs and dopamine. More importantly, there is a critical value of polydopamine shell thickness for the maximum rebinding capacity. The as-prepared lysozyme-imprinted Fe3O4@polydopamine NPs show high binding capacity and acceptable specific recognition behavior towards template proteins. This method provides the possibility for the separation and enrichment of abundant proteins in proteomic analysis.
语种英语
WOS记录号WOS:000303604000024
引用统计
被引频次:182[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/118171
专题中国科学院大连化学物理研究所
通讯作者Chen LX(陈朗星)
作者单位1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
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GB/T 7714
Zhang, Min,Zhang, Xihao,He, Xiwen,et al. A self-assembled polydopamine film on the surface of magnetic nanoparticles for specific capture of protein[J]. NANOSCALE,2012,4(10):3141-3147.
APA Zhang, Min,Zhang, Xihao,He, Xiwen,Chen, Langxing,Zhang, Yukui,&陈朗星.(2012).A self-assembled polydopamine film on the surface of magnetic nanoparticles for specific capture of protein.NANOSCALE,4(10),3141-3147.
MLA Zhang, Min,et al."A self-assembled polydopamine film on the surface of magnetic nanoparticles for specific capture of protein".NANOSCALE 4.10(2012):3141-3147.
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