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学科主题: 物理化学
题名: A compact and highly sensitive light-emitting diode-induced fluorescence detector for capillary flow systems
作者: Geng, Xuhui1, 2;  Wu, Dapeng1;  Guan, Yafeng1
通讯作者: 关亚风
关键词: Light-emitting diode ;  Fluorescence detector ;  Compact
刊名: TALANTA
发表日期: 2012-01-15
DOI: 10.1016/j.talanta.2011.11.015
卷: 88, 期:15, 页:463-467
收录类别: SCI
文章类型: Article
部门归属: 1
项目归属: 105
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A compact and highly sensitive light-emitting diode-induced fluorescence detector (LED-FD) was constructed and evaluated. The LED excitation beam was restricted by a 200 mu m diameter pinhole on a 55 mu m thick lighttight film. The LED, excitation filter, pinhole, and capillary flow cell were placed tightly against each other without either optical lens or excitation fiber. The performance of the LED-FD was evaluated by flow injection analysis (FIA) of sodium fluorescein solution. The limit of detection (LOD) was determined as 0.75 nM (SNR= 3). Compared with our previous work, a 3.5-fold enhancement on the signal-to-noise ratio (SNR) was obtained. The system exhibited linear responses in the concentration range of 2-200 nM (R = 0.9993). The error of repeatability was within 2.0% R.S.D. on peak height in a ten-repeated injection of 5 nM solution. The reproducibility between devices was within 2.2% R.S.D. on peak height of 10 nM fluorescein solution. Finally, the LED-FD was compared with commercial fluorescence detector, and similar fluorescence signal intensity was obtained. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: OPTICAL-FIBER ;  ELECTROPHORESIS ;  PERFORMANCE
语种: 英语
WOS记录号: WOS:000301159400064
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118224
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Geng, Xuhui,Wu, Dapeng,Guan, Yafeng. A compact and highly sensitive light-emitting diode-induced fluorescence detector for capillary flow systems[J]. TALANTA,2012,88(15):463-467.
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