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学科主题: 物理化学
题名: Preparation of capillary hybrid monolithic column with sulfonate strong cation exchanger for proteome analysis
作者: Zhang, Zhenbin1, 2;  Wang, Fangjun1;  Xu, Bo1, 2;  Qin, Hongqiang1, 2;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 邹汉法
关键词: Hybrid monolithic column ;  Strong cation exchange ;  Proteome analysis ;  Phosphopeptide ;  Enzyme reactor
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2012-09-21
DOI: 10.1016/j.chroma.2012.07.071
卷: 1256, 页:136-143
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1,1
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Strong cation exchange (SCX) chromatography is one of the most important separation modes in liquid chromatography and SCX column is widely applied in high resolution separation or fractionation of various samples. In this work, a sulfonate SCX hybrid monolithic column was successfully prepared by "one-pot" strategy and the hybrid monolith is well optimized to obtain homogenous structure. It was observed that this sulfonate SCX hybrid monolithic column had similar to 7 times permeability (in water) and similar to 3 times sample loading capacity (tested by dipeptide Gly-Tyr) comparing to particulate SCX column packed with commercial available material. Then, it was used as trap column for fast sample loading of the enriched phosphopeptides. Comparing to phosphate SCX polymer monolithic column, the number of identified phosphopeptides increased similar to 19% due to the sulfonate group has higher retention strength than phosphate group for peptide cations. And the coverage of phosphoproteome obtained by sulfonate SCX hybrid monolithic column is similar to particulate packed SCX column, because they had identical sulfonate group to retain the peptide cations. Finally, the sulfonate SCX hybrid monolithic column was used as enzyme reactor for online protein digestion. Comparing to particulate SCX packed column, the identified peptides number increased 40% and the protein coverage increased 10%. This might be ascribed to the high porous structure and relative high surface area that elevated the digestion efficiency. (C) 2012 Elsevier B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  PHOSPHOPROTEOME ANALYSIS ;  IDENTIFICATION TECHNOLOGY ;  YEAST PROTEOME ;  TRAP COLUMN ;  SEPARATION ;  ELECTROCHROMATOGRAPHY ;  PEPTIDES ;  SAMPLES
语种: 英语
WOS记录号: WOS:000308624000018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118251
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Zhang, Zhenbin,Wang, Fangjun,Xu, Bo,et al. Preparation of capillary hybrid monolithic column with sulfonate strong cation exchanger for proteome analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1256:136-143.
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