DICP OpenIR
Subject Area物理化学
Preparation of capillary hybrid monolithic column with sulfonate strong cation exchanger for proteome analysis
Zhang, Zhenbin1,2; Wang, Fangjun1; Xu, Bo1,2; Qin, Hongqiang1,2; Ye, Mingliang1; Zou, Hanfa1; Zou HF(邹汉法)
KeywordHybrid Monolithic Column Strong Cation Exchange Proteome Analysis Phosphopeptide Enzyme Reactor
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2012-09-21
ISSN0021-9673
DOI10.1016/j.chroma.2012.07.071
Volume1256Pages:136-143
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1,1
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; PHOSPHOPROTEOME ANALYSIS ; IDENTIFICATION TECHNOLOGY ; YEAST PROTEOME ; TRAP COLUMN ; SEPARATION ; ELECTROCHROMATOGRAPHY ; PEPTIDES ; SAMPLES
AbstractStrong cation exchange (SCX) chromatography is one of the most important separation modes in liquid chromatography and SCX column is widely applied in high resolution separation or fractionation of various samples. In this work, a sulfonate SCX hybrid monolithic column was successfully prepared by "one-pot" strategy and the hybrid monolith is well optimized to obtain homogenous structure. It was observed that this sulfonate SCX hybrid monolithic column had similar to 7 times permeability (in water) and similar to 3 times sample loading capacity (tested by dipeptide Gly-Tyr) comparing to particulate SCX column packed with commercial available material. Then, it was used as trap column for fast sample loading of the enriched phosphopeptides. Comparing to phosphate SCX polymer monolithic column, the number of identified phosphopeptides increased similar to 19% due to the sulfonate group has higher retention strength than phosphate group for peptide cations. And the coverage of phosphoproteome obtained by sulfonate SCX hybrid monolithic column is similar to particulate packed SCX column, because they had identical sulfonate group to retain the peptide cations. Finally, the sulfonate SCX hybrid monolithic column was used as enzyme reactor for online protein digestion. Comparing to particulate SCX packed column, the identified peptides number increased 40% and the protein coverage increased 10%. This might be ascribed to the high porous structure and relative high surface area that elevated the digestion efficiency. (C) 2012 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000308624000018
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/118251
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Zhenbin,Wang, Fangjun,Xu, Bo,et al. Preparation of capillary hybrid monolithic column with sulfonate strong cation exchanger for proteome analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1256:136-143.
APA Zhang, Zhenbin.,Wang, Fangjun.,Xu, Bo.,Qin, Hongqiang.,Ye, Mingliang.,...&邹汉法.(2012).Preparation of capillary hybrid monolithic column with sulfonate strong cation exchanger for proteome analysis.JOURNAL OF CHROMATOGRAPHY A,1256,136-143.
MLA Zhang, Zhenbin,et al."Preparation of capillary hybrid monolithic column with sulfonate strong cation exchanger for proteome analysis".JOURNAL OF CHROMATOGRAPHY A 1256(2012):136-143.
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